Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii.

abstract：:In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

journal_title：Plant molecular biology

authors： van Tunen AJ,Hartman SA,Mur LA,Mol JN

更新日期：1989-05-01 00:00:00

abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

journal_title：Plant molecular biology

authors： Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

更新日期：2004-05-01 00:00:00

abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...

journal_title：Plant molecular biology

authors： Kao CW,Bakshi M,Sherameti I,Dong S,Reichelt M,Oelmüller R,Yeh KW

更新日期：2016-12-01 00:00:00

abstract：:Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...

journal_title：Plant molecular biology

authors： He Y,Gan S

更新日期：2004-01-01 00:00:00

abstract：:Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

journal_title：Plant molecular biology

authors： Burow MD,Chen ZY,Mouton TM,Moroney JV

更新日期：1996-05-01 00:00:00

abstract：:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

journal_title：Plant molecular biology

authors： Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

更新日期：2002-05-01 00:00:00

abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...

journal_title：Plant molecular biology

authors： Müller BS,Sakamoto T,de Menezes IP,Prado GS,Martins WS,Brondani C,de Barros EG,Vianello RP

更新日期：2014-11-01 00:00:00

abstract：:A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...

journal_title：Plant molecular biology

authors： Hsieh HL,Tong CG,Thomas C,Roux SJ

更新日期：1996-01-01 00:00:00

abstract：:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

journal_title：Plant molecular biology

authors： Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

更新日期：2001-02-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...

journal_title：Plant molecular biology

authors： Chung KM,Sano H

更新日期：2007-12-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

journal_title：Plant molecular biology

authors： Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

更新日期：2003-06-01 00:00:00

abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

journal_title：Plant molecular biology

authors： de Jager SM,Menges M,Bauer UM,Murra JA

更新日期：2001-11-01 00:00:00

abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

journal_title：Plant molecular biology

authors： Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

更新日期：1991-10-01 00:00:00

abstract：:Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sit...

journal_title：Plant molecular biology

authors： Uemukai K,Iwakawa H,Kosugi S,de Uemukai S,Kato K,Kondorosi E,Murray JA,Ito M,Shinmyo A,Sekine M

更新日期：2005-01-01 00:00:00

abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

journal_title：Plant molecular biology

authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

更新日期：2008-11-01 00:00:00

abstract：:Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

journal_title：Plant molecular biology

authors： Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

更新日期：2011-11-01 00:00:00

abstract：:cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...

journal_title：Plant molecular biology

authors： Fukuchi-Mizutani M,Savin K,Cornish E,Tanaka Y,Ashikari T,Kusumi T,Murata N

更新日期：1995-11-01 00:00:00

abstract：:Galactomannans are hemicellulosic polysaccharides composed of a (1 → 4)-linked β-D-mannan backbone substituted with single-unit (1 → 6)-α-linked D-galactosyl residues. Developing fenugreek (Trigonella foenum-graecum) seeds are known to accumulate large quantities of galactomannans in the endosperm, and were thus used ...

journal_title：Plant molecular biology

authors： Wang Y,Alonso AP,Wilkerson CG,Keegstra K

更新日期：2012-06-01 00:00:00

abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

journal_title：Plant molecular biology

authors： Adamska I,Funk C,Renger G,Andersson B

更新日期：1996-07-01 00:00:00

abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...

journal_title：Plant molecular biology

authors： Maiti MK,Krishnasamy S,Owen HA,Makaroff CA

更新日期：1997-11-01 00:00:00

abstract：:The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...

journal_title：Plant molecular biology

authors： Fukuda Y,Shinshi H

更新日期：1994-02-01 00:00:00

abstract：:A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...

journal_title：Plant molecular biology

authors： Espartero J,Sánchez-Aguayo I,Pardo JM

更新日期：1995-12-01 00:00:00

abstract：:The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...

journal_title：Plant molecular biology

authors： Cutanda-Perez MC,Ageorges A,Gomez C,Vialet S,Terrier N,Romieu C,Torregrosa L

更新日期：2009-04-01 00:00:00

abstract：:StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...

journal_title：Plant molecular biology

authors： Gargantini PR,Giammaria V,Grandellis C,Feingold SE,Maldonado S,Ulloa RM

更新日期：2009-05-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：:Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...

journal_title：Plant molecular biology

authors： Newton SS,Duman JG

更新日期：2000-11-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:Target sequence cleavage is the essential step for intron invasion into an intronless allele. DNA cleavage at a specific site is performed by an endonuclease, termed a homing enzyme, which is encoded by an open reading frame within the intron. The recognition properties of them have only been analyzed in vitro, using ...

journal_title：Plant molecular biology

authors： Kurokawa S,Yamasaki T,Komatsu T,Watanabe KI,Ohama T

更新日期：2006-09-01 00:00:00

abstract：:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...

journal_title：Plant molecular biology

authors： Kuroda I,Inagaki J,Yamamoto Y

更新日期：1993-01-01 00:00:00