Abstract:
:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with the powdery mildew fungus. We have isolated a cDNA encoding a plasma membrane H+-ATPase (HvHAI), which is also induced by powdery mildew attack. The C-terminal domain of this H+-ATPase interacts with 14-3-3 proteins in the yeast two-hybrid system. Inoculation with the powdery mildew fungus, or treatment with fusicoccin, results in an increase in fusicoccin binding ability of barley leaf membranes. Overlay assays show a fungus-induced increase in binding of digoxygenin-labelled 14-3-3 protein to several proteins including a 100 kDa membrane protein, probably the plasma membrane H+-ATPase. These effects are seen specifically in the inoculated epidermis and not in the whole leaf. We propose that 14-3-3 proteins are involved in an epidermis-specific response to the powdery mildew fungus, possibly via an activation of the plasma membrane H+-ATPase.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DBdoi
10.1023/a:1014938417267subject
Has Abstractpub_date
2002-05-01 00:00:00pages
137-47issue
2eissn
0167-4412issn
1573-5028journal_volume
49pub_type
杂志文章abstract::Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00991-3
更新日期:2020-06-01 00:00:00
abstract::Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1013644026132
更新日期:2001-12-01 00:00:00
abstract::A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005816104743
更新日期:1997-06-01 00:00:00
abstract::Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01578377
更新日期:1983-07-01 00:00:00
abstract::Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9458-1
更新日期:2009-05-01 00:00:00
abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005888616748
更新日期:1997-07-01 00:00:00
abstract::Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019389
更新日期:1990-06-01 00:00:00
abstract::The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049336
更新日期:1996-02-01 00:00:00
abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018558
更新日期:1990-02-01 00:00:00
abstract::Many chloroplast proteins are synthesized in the cytoplasm as precursors which contain an amino terminal transit peptide. These precursors are subsequently imported into chloroplast and targeted to one of several organellar locations. This import is mediated by the transit peptide, which is cleaved off during import. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014912
更新日期:1987-07-01 00:00:00
abstract::The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by anoth...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021787
更新日期:1996-05-01 00:00:00
abstract::The formation and the development of the floral organs require an intercalate expression of organ-specific genes. At the same time, meristem-specific genes are repressed to complete the differentiation of the organs in the floral whorls. In an Arabidopsis activation tagging population, a mutant affected in inflorescen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-0698-4
更新日期:2005-03-01 00:00:00
abstract::It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499832424
更新日期:2000-06-01 00:00:00
abstract::Hazelnut (Corylus avellana L.) is a species of economic interest that shows a peculiar floral biology. Unlike most of the angiosperms, which produce ovules during floral development such that they are ready for pollen at anthesis, hazelnut ovary development is delayed and triggered by compatible pollination. In order ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006022524708
更新日期:1998-12-01 00:00:00
abstract::Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0187-8
更新日期:2014-06-01 00:00:00
abstract::To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
abstract::The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024116
更新日期:1994-02-01 00:00:00
abstract::Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021532
更新日期:1993-11-01 00:00:00
abstract::Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006498413543
更新日期:2000-09-01 00:00:00
abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028878
更新日期:1994-08-01 00:00:00
abstract::As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0239-0
更新日期:2014-11-01 00:00:00
abstract::In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014970
更新日期:1995-12-01 00:00:00
abstract::Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016064
更新日期:1984-05-01 00:00:00
abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015971
更新日期:1993-06-01 00:00:00
abstract::The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006419232075
更新日期:2000-05-01 00:00:00
abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016019
更新日期:1989-12-01 00:00:00
abstract::A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020195
更新日期:1995-01-01 00:00:00
abstract::We isolated and characterized different classes of transposable DNA elements in oil palm (Elaeis guineensis) plants grown from seed, and plants regenerated from tissue culture that show mantling, an abnormality leading to flower abortion. Using PCR assays, reverse transcriptase fragments belonging to LINE-like and gyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023942309092
更新日期:2003-05-01 00:00:00
abstract::Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006196530079
更新日期:1999-05-01 00:00:00