Abstract:
:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL region of the biotype I octopine strain Ach5 and 'small TA' strains with a similar T region organization as the 'large TA' strains but with a large internal TA deletion. Structural and functional studies of the representative 'large TA' strain Tm4 revealed six oncogenes. Each oncogene was inserted in a disarmed vector and tested for biological activity using the corresponding oncogenes of Ach5 as standards. Five Tm4 oncogenes, TA-iaaM, T-ipt, T-6b, TB-iaaH and TB-iaaM, were shown to be active, the IS-interrupted TA-iaaH gene was inactive. To study the role of each gene in the pTiTm4 context, several single and multiple pTiTm4 mutations were constructed. It was shown that whereas TA-iaaM and TB-iaaH are essential for tumour formation on grapevine, T-ipt, T-6b and TB-iaaM are not. The avirulence of the TA-iaaM- mutant was shown to be due to an inhibitory effect of the T-ipt gene, since a TA-iaaM-/T-ipt- double mutant was fully virulent. We conclude that the TA-iaaM gene of 'large TA' strains is specifically required to counteract the tumour growth inhibiting activity of the T-ipt gene. Both TA-iaaM and T-ipt are absent from the 'small TA' strains. A model on the roles and interactions of the different oncogenes in 'large TA' and 'small TA' strains is presented.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Huss B,Tinland B,Paulus F,Walter B,Otten Ldoi
10.1007/BF00018558subject
Has Abstractpub_date
1990-02-01 00:00:00pages
173-86issue
2eissn
0167-4412issn
1573-5028journal_volume
14pub_type
杂志文章abstract:KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0516-1
更新日期:2016-10-01 00:00:00
abstract::The complete nucleotide sequence of the Chlamydomonas eugametos (Chlamydomonadales, Chlorophyceae, sensu Mattox and Stewart) mitochondrial genome has been determined (22,897 bp, 34.6% G + C). The genes identified in this circular-mapping genome include those for apocytochrome b, subunit 1 of the cytochrome oxidase com...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005995718091
更新日期:1998-01-01 00:00:00
abstract::A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein was isolated from cold-treated barley leaves combining the differential display and the 5'-RACE techniques. Cor tmc-ap3 is expressed at low level under normal growing temperature, and its expression is strongly...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375332677
更新日期:1999-09-01 00:00:00
abstract::The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000004333.96451.11
更新日期:2003-08-01 00:00:00
abstract::The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0350-x
更新日期:2015-08-01 00:00:00
abstract::Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-1004-1
更新日期:2005-11-01 00:00:00
abstract::The microtubular element of the plant cytoskeleton undergoes dramatic architectural changes in the course of the cell cycle, specifically at the entry into and exit from mitosis. These changes underlie the acquisition of specialized properties and functions involved, for example, in the equal segregation of chromosome...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006346107807
更新日期:2000-08-01 00:00:00
abstract:KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0581-5
更新日期:2017-04-01 00:00:00
abstract::Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006299821980
更新日期:1999-05-01 00:00:00
abstract::Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9273-5
更新日期:2008-03-01 00:00:00
abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020254
更新日期:1995-05-01 00:00:00
abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000019059.56489.ca
更新日期:2003-11-01 00:00:00
abstract::Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499808317
更新日期:2000-06-01 00:00:00
abstract::Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9784-y
更新日期:2011-07-01 00:00:00
abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020199
更新日期:1996-11-01 00:00:00
abstract::The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00032673
更新日期:1995-09-01 00:00:00
abstract::To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010614522706
更新日期:2001-07-01 00:00:00
abstract:KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01064-1
更新日期:2020-12-01 00:00:00
abstract::We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000006936.50532.3a
更新日期:2003-10-01 00:00:00
abstract::Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9092-0
更新日期:2007-02-01 00:00:00
abstract::The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017779
更新日期:1984-09-01 00:00:00
abstract::Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-06-01 00:00:00
abstract:KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00884-0
更新日期:2019-08-01 00:00:00
abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0526-z
更新日期:2016-11-01 00:00:00
abstract::In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0244-3
更新日期:2014-11-01 00:00:00
abstract::A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017808
更新日期:1996-01-01 00:00:00
abstract:KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0595-7
更新日期:2017-05-01 00:00:00
abstract::Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029857
更新日期:1994-03-01 00:00:00
abstract::Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9023-0
更新日期:2006-10-01 00:00:00
abstract::The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0459-6
更新日期:2016-06-01 00:00:00