High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals.

abstract：:Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

journal_title：Plant molecular biology

authors： Romano CP,Robson PR,Smith H,Estelle M,Klee H

更新日期：1995-03-01 00:00:00

abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

journal_title：Plant molecular biology

authors： Adamska I,Funk C,Renger G,Andersson B

更新日期：1996-07-01 00:00:00

abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...

journal_title：Plant molecular biology

authors： Cervantes E,Rodríguez A,Nicolás G

更新日期：1994-05-01 00:00:00

abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...

journal_title：Plant molecular biology

authors： Severin K,Schöffl F

更新日期：1990-12-01 00:00:00

abstract：:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...

journal_title：Plant molecular biology

authors： Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

更新日期：2006-05-01 00:00:00

abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

journal_title：Plant molecular biology

authors： Wei T,O'Connell MA

更新日期：1996-01-01 00:00:00

abstract：:The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

journal_title：Plant molecular biology

authors： Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

更新日期：1993-03-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00

abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

journal_title：Plant molecular biology

authors： Devitt ML,Maas KJ,Stafstrom JP

更新日期：1999-01-01 00:00:00

abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...

journal_title：Plant molecular biology

authors： Zhao X,Kochert G

更新日期：1993-02-01 00:00:00

abstract：KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...

journal_title：Plant molecular biology

authors： Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

更新日期：2021-01-22 00:00:00

abstract：:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...

journal_title：Plant molecular biology

authors： Kim MJ,Ham BK,Kim HR,Lee IJ,Kim YJ,Ryu KH,Park YI,Paek KH

更新日期：2005-12-01 00:00:00

abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

journal_title：Plant molecular biology

authors： Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

更新日期：2006-08-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...

journal_title：Plant molecular biology

authors： Albani D,Parisi B,Carbonera D,Cella R

更新日期：1985-11-01 00:00:00

abstract：:A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...

journal_title：Plant molecular biology

authors： Siemeister G,Hachtel W

更新日期：1990-05-01 00:00:00

abstract：:Angiosperm genomes show tremendous variability in genome size and chromosome number. Nevertheless, comparative genetic mapping has revealed genome collinearity of closely related species. Sequence-based comparisons were used to assess the conservation of gene arrangements. Numerous small rearrangements, insertions/del...

journal_title：Plant molecular biology

authors： Schmidt R

更新日期：2002-01-01 00:00:00

abstract：KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

journal_title：Plant molecular biology

authors： Geilfus CM,Lan J,Carpentier S

更新日期：2018-12-01 00:00:00

abstract：:Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...

journal_title：Plant molecular biology

authors： Vogel H,Fischer S,Valentin K

更新日期：1996-11-01 00:00:00

abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

journal_title：Plant molecular biology

authors： Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

更新日期：2011-10-01 00:00:00

abstract：:An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...

journal_title：Plant molecular biology

authors： Zhang S,Gu YQ,Singh J,Coleman-Derr D,Brar DS,Jiang N,Lemaux PG

更新日期：2007-07-01 00:00:00

abstract：:Plant S-adenosyl-L-methionine-dependent methyltransferases (SAM-Mtases) are the key enzymes in phenylpropanoid, flavonoid and many other metabolic pathways of biotechnological importance. Here we compiled the amino acid sequences of 56 SAM-Mtases from different plants and performed a computer analysis for the conserve...

journal_title：Plant molecular biology

authors： Joshi CP,Chiang VL

更新日期：1998-07-01 00:00:00

abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

journal_title：Plant molecular biology

authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

更新日期：1997-05-01 00:00:00

abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

journal_title：Plant molecular biology

authors： Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

更新日期：2017-04-01 00:00:00

abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...

journal_title：Plant molecular biology

authors： Palauqui JC,Vaucheret H

更新日期：1995-10-01 00:00:00

abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...

journal_title：Plant molecular biology

authors： Yuan C,Huang D,Yang Y,Sun M,Cheng T,Wang J,Pan H,Zhang Q

更新日期：2020-05-01 00:00:00

abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

journal_title：Plant molecular biology

authors： Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

更新日期：2004-05-01 00:00:00

abstract：:We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

journal_title：Plant molecular biology

authors： Carballo M,Giné R,Santos M,Puigdomènech P

更新日期：1991-01-01 00:00:00

abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

journal_title：Plant molecular biology

authors： Atanassova R,Flénet M,Gigot C,Chaubet N

更新日期：1998-05-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00