Field trial analysis of nitrate reductase co-suppression: a comparative study of 38 combinations of transgene loci.

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

journal_title：Plant molecular biology

authors： Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

更新日期：1999-05-01 00:00:00

abstract：:Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

journal_title：Plant molecular biology

authors： Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

更新日期：1986-07-01 00:00:00

abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

journal_title：Plant molecular biology

authors： Adamska I,Funk C,Renger G,Andersson B

更新日期：1996-07-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...

journal_title：Plant molecular biology

authors： McNay JW,Pring DR,Lonsdale DM

更新日期：1983-07-01 00:00:00

abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

journal_title：Plant molecular biology

authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

更新日期：2003-05-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:The nucleotide sequence and the 5' flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and the deduced amino-acid sequence has been compared to those of various photosynthetic and chemoautotrophic Eu...

journal_title：Plant molecular biology

authors： Assali NE,Mache R,Loiseaux-de Goër S

更新日期：1990-08-01 00:00:00

abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

journal_title：Plant molecular biology

authors： Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

更新日期：1995-11-01 00:00:00

abstract：:Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...

journal_title：Plant molecular biology

authors： Li AL,Zhu YF,Tan XM,Wang X,Wei B,Guo HZ,Zhang ZL,Chen XB,Zhao GY,Kong XY,Jia JZ,Mao L

更新日期：2008-03-01 00:00:00

abstract：:The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

journal_title：Plant molecular biology

authors： Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

更新日期：1993-03-01 00:00:00

abstract：:In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...

journal_title：Plant molecular biology

authors： Sessa G,Fluhr R

更新日期：1995-12-01 00:00:00

abstract：:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...

journal_title：Plant molecular biology

authors： Kuroda I,Inagaki J,Yamamoto Y

更新日期：1993-01-01 00:00:00

abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...

journal_title：Plant molecular biology

authors： Maiti MK,Krishnasamy S,Owen HA,Makaroff CA

更新日期：1997-11-01 00:00:00

abstract：:Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...

journal_title：Plant molecular biology

authors： Sandhu D,Gill KS

更新日期：2002-03-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...

journal_title：Plant molecular biology

authors： Chung KM,Sano H

更新日期：2007-12-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...

journal_title：Plant molecular biology

authors： Fukuda Y,Shinshi H

更新日期：1994-02-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

journal_title：Plant molecular biology

authors： Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

更新日期：1999-07-01 00:00:00

abstract：:RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

journal_title：Plant molecular biology

authors： Tang F,Yang S,Gao M,Zhu H

更新日期：2013-07-01 00:00:00

abstract：:Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...

journal_title：Plant molecular biology

authors： Qiu LJ,Xing LL,Guo Y,Wang J,Jackson SA,Chang RZ

更新日期：2013-09-01 00:00:00

abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...

journal_title：Plant molecular biology

authors： Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L

更新日期：1991-09-01 00:00:00

abstract：:Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sit...

journal_title：Plant molecular biology

authors： Uemukai K,Iwakawa H,Kosugi S,de Uemukai S,Kato K,Kondorosi E,Murray JA,Ito M,Shinmyo A,Sekine M

更新日期：2005-01-01 00:00:00

abstract：:A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...

journal_title：Plant molecular biology

authors： Vignols F,José-Estanyol M,Caparrós-Ruiz D,Rigau J,Puigdomènech P

更新日期：1999-03-01 00:00:00

abstract：:Plant S-adenosyl-L-methionine-dependent methyltransferases (SAM-Mtases) are the key enzymes in phenylpropanoid, flavonoid and many other metabolic pathways of biotechnological importance. Here we compiled the amino acid sequences of 56 SAM-Mtases from different plants and performed a computer analysis for the conserve...

journal_title：Plant molecular biology

authors： Joshi CP,Chiang VL

更新日期：1998-07-01 00:00:00

abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

journal_title：Plant molecular biology

authors： Lertpiriyapong K,Sung ZR

更新日期：2003-11-01 00:00:00

abstract：:The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...

journal_title：Plant molecular biology

authors： Moon H,Chowrira G,Rowland O,Blacklock BJ,Smith MA,Kunst L

更新日期：2004-12-01 00:00:00

abstract：:The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...

journal_title：Plant molecular biology

authors： Mirabella R,Martirani L,Lamberti A,Iaccarino M,Chiurazzi M

更新日期：1999-01-01 00:00:00