Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein.

Abstract:

:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Kuroda I,Inagaki J,Yamamoto Y

doi

10.1007/BF00039627

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

171-6

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

21

pub_type

杂志文章
  • Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers.

    abstract::Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1024822312038

    authors: Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

    更新日期:2003-06-01 00:00:00

  • Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

    abstract::In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028867

    authors: Conceição Ada S,Van Vliet A,Krebbers E

    更新日期:1994-11-01 00:00:00

  • Functional analysis of a complex oncogene arrangement in biotype III Agrobacterium tumefaciens strains.

    abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00018558

    authors: Huss B,Tinland B,Paulus F,Walter B,Otten L

    更新日期:1990-02-01 00:00:00

  • Cytoplasmic male sterility in sunflower: comparison of molecular biology and genetic studies.

    abstract::The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023992

    authors: Crouzillat D,de la Canal L,Perrault A,Ledoigt G,Vear F,Serieys H

    更新日期:1991-03-01 00:00:00

  • A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine.

    abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000009269.97773.70

    authors: Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama K

    更新日期:2003-09-01 00:00:00

  • Transcriptional activation of tobacco E2F is repressed by co-transfection with the retinoblastoma-related protein: cyclin D expression overcomes this repressor activity.

    abstract::Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sit...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-6601-x

    authors: Uemukai K,Iwakawa H,Kosugi S,de Uemukai S,Kato K,Kondorosi E,Murray JA,Ito M,Shinmyo A,Sekine M

    更新日期:2005-01-01 00:00:00

  • Characterization of pectinases and pectin methylesterase cDNAs in pods of green beans (Phaseolus vulgaris L.).

    abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040831

    authors: Ebbelaar ME,Tucker GA,Laats MM,van Dijk C,Stolle-Smits T,Recourt K

    更新日期:1996-09-01 00:00:00

  • Alternative splicing is required for RCT1-mediated disease resistance in Medicago truncatula.

    abstract::RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0068-6

    authors: Tang F,Yang S,Gao M,Zhu H

    更新日期:2013-07-01 00:00:00

  • Characterization and comparison of intron structure and alternative splicing between Medicago truncatula, Populus trichocarpa, Arabidopsis and rice.

    abstract::Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9334-4

    authors: Baek JM,Han P,Iandolino A,Cook DR

    更新日期:2008-07-01 00:00:00

  • Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status: stereospecific down-regulation by L-cysteine.

    abstract::To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006148815106

    authors: Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

    更新日期:1999-02-01 00:00:00

  • Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.

    abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-0393-x

    authors: Ogita S,Uefuji H,Morimoto M,Sano H

    更新日期:2004-04-01 00:00:00

  • Chloroplast transcription is required to express the nuclear genes rbcS and cab. Plastid DNA copy number is regulated independently.

    abstract::RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00037063

    authors: Rapp JC,Mullet JE

    更新日期:1991-10-01 00:00:00

  • A maize histone deacetylase and retinoblastoma-related protein physically interact and cooperate in repressing gene transcription.

    abstract::In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1022090916446

    authors: Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

    更新日期:2003-02-01 00:00:00

  • Uncovering the post-embryonic role of embryo essential genes in Arabidopsis using the controlled induction of visibly marked genetic mosaics: EMB506, an illustration.

    abstract::The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-6268-6

    authors: Latvala-Kilby SM,Kilby NJ

    更新日期:2006-05-01 00:00:00

  • Characterization of the expression of a wheat cystatin gene during caryopsis development.

    abstract::A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-termin...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1019906031305

    authors: Corr-Menguy F,Cejudo FJ,Mazubert C,Vidal J,Lelandais-Brière C,Torres G,Rode A,Hartmann C

    更新日期:2002-11-01 00:00:00

  • Novel rice mutants overexpressing the brassinosteroid catabolic gene CYP734A4.

    abstract:KEY MESSAGE:Moderate overexpression of CYP734A4 improves grain number per main panicle and seed setting rate. Brassinosteroid (BR) homeostasis and signaling are crucial for plant growth and development. CYP734A genes encode cytochrome P450 monooxygenases that control the level of bioactive BRs by degrading BRs. However...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0558-4

    authors: Qian W,Wu C,Fu Y,Hu G,He Z,Liu W

    更新日期:2017-01-01 00:00:00

  • Nutritional functions of the funiculus in Brassica napus seed maturation revealed by transcriptome and dynamic metabolite profile analyses.

    abstract::The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0530-3

    authors: Tan H,Xiang X,Tang J,Wang X

    更新日期:2016-11-01 00:00:00

  • Distinct reorganization of the genome transcription associates with organogenesis of somatic embryo, shoots, and roots in rice.

    abstract::Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9092-0

    authors: Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

    更新日期:2007-02-01 00:00:00

  • Molecular characterization and expression study of a histidine auxotrophic mutant (his1-) of Nicotiana plumbaginifolia.

    abstract::The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006493021557

    authors: El Malki F,Jacobs M

    更新日期:2001-01-01 00:00:00

  • Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice.

    abstract::We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-007-9284-2

    authors: Kanneganti V,Gupta AK

    更新日期:2008-03-01 00:00:00

  • Profiling the expression of genes controlling rice grain quality.

    abstract::Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-7507-3

    authors: Duan M,Sun SS

    更新日期:2005-09-01 00:00:00

  • Differential expression of two calmodulin genes in response to physical and chemical stimuli.

    abstract::Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00029857

    authors: Botella JR,Arteca RN

    更新日期:1994-03-01 00:00:00

  • Characterization of a pine multigene family containing elicitor-responsive stilbene synthase genes.

    abstract::Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006163030646

    authors: Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

    更新日期:1999-01-01 00:00:00

  • A novel TaMYB4 transcription factor involved in the defence response against Puccinia striiformis f. sp. tritici and abiotic stresses.

    abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0156-7

    authors: Al-Attala MN,Wang X,Abou-Attia MA,Duan X,Kang Z

    更新日期:2014-03-01 00:00:00

  • Expression of one of the members of the Arabidopsis chaperonin 60 beta gene family is developmentally regulated and wound-repressible.

    abstract::To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040585

    authors: Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

    更新日期:1994-01-01 00:00:00

  • Genetic linkage facilitates cloning of Ert-m regulating plant architecture in barley and identified a strong candidate of Ant1 involved in anthocyanin biosynthesis.

    abstract::The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0350-x

    authors: Zakhrabekova S,Dockter C,Ahmann K,Braumann I,Gough SP,Wendt T,Lundqvist U,Mascher M,Stein N,Hansson M

    更新日期:2015-08-01 00:00:00

  • The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum.

    abstract::The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019395

    authors: Kato A,Nakajima T,Yamashita J,Yakura K,Tanifuji S

    更新日期:1990-06-01 00:00:00

  • Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene.

    abstract::Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00038998

    authors: Johanningmeier U,Heiss S

    更新日期:1993-04-01 00:00:00

  • Long-range physical maps of two loci (Aps-1 and GP79) flanking the root-knot nematode resistance gene (Mi) near the centromere of tomato chromosome 6.

    abstract::The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021430

    authors: van Daelen RA,Gerbens F,van Ruissen F,Aarts J,Hontelez J,Zabel P

    更新日期:1993-10-01 00:00:00

  • Nucleotide sequence of two cDNAs encoding fucoxanthin chlorophyll a/c proteins in the diatom Odontella sinensis.

    abstract::Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020236

    authors: Kroth-Pancic PG

    更新日期:1995-02-01 00:00:00