Abstract:
:The nucleotide sequence and the 5' flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and the deduced amino-acid sequence has been compared to those of various photosynthetic and chemoautotrophic Eubacteria, of a red alga and of green plastids (Euglena gracilis, green algae and higher plants). Unlike the rbcL genes of green plastids which are more closely related to those of cyanobacteria, the P. littoralis rbcL gene is more closely related to that of a beta-purple bacterium, as was found for the rbcS gene of another chromophytic alga [Boczar et al., Proc Natl Acad Sci USA 86: 4996-4999, 1989]. Matrix data of homology between the rbcL gene of P. littoralis and the same gene of other organisms are presented. Based on our previous report, the gene coding for the 16S rRNA from P. littoralis is closely related to that of E. gracilis (Markowicz et al., Curr Genet 14: 599-608, 1988). We suggest that the large plastid DNA molecule of P. littoralis is a phylogenetically composite genome which probably resulted from mixed endosymbiosis events, or from a horizontal transfer of DNA.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Assali NE,Mache R,Loiseaux-de Goër Sdoi
10.1007/BF00036916subject
Has Abstractpub_date
1990-08-01 00:00:00pages
307-15issue
2eissn
0167-4412issn
1573-5028journal_volume
15pub_type
杂志文章abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026599028063
更新日期:2000-11-01 00:00:00
abstract::The isolation of cDNAs is described which encode the complete sequence of a precursor protein for a HSP90 homologue consisting of an N-terminal transit peptide of 5850 Da and a mature protein (cpHSP82) of 82 260 Da, located in the plastids of rye leaves (Secale cereale). Hybridization analysis indicated the presence o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049326
更新日期:1996-02-01 00:00:00
abstract::We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3249-5
更新日期:2004-09-01 00:00:00
abstract::The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9138-y
更新日期:2007-05-01 00:00:00
abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
abstract::Annexins belong to a multigene family of Ca(2+) dependent, phospholipid and cytoskeleton binding proteins. They have been shown to be upregulated under various stress conditions. We generated transgenic cotton plants expressing mustard annexin (AnnBj1), which showed enhanced tolerance towards different abiotic stress ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9615-6
更新日期:2010-06-01 00:00:00
abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9428-z
更新日期:2009-02-01 00:00:00
abstract::When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020185
更新日期:1995-01-01 00:00:00
abstract::Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000009296.91149.34
更新日期:2003-09-01 00:00:00
abstract::Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017450
更新日期:1989-01-01 00:00:00
abstract::Two S-adenosylmethionine synthetase (SAMS) cDNAs, PcSAMS1 and PcSAMS2, have been identified in Pinus contorta. We found that the two genes are differentially expressed during root development. Thus, PcSAMS1 is preferentially expressed in roots and exhibits a specific expression pattern in the meristem at the onset of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010637012528
更新日期:2001-06-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1021162321527
更新日期:2003-01-01 00:00:00
abstract::A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006031822141
更新日期:1998-07-01 00:00:00
abstract::A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017808
更新日期:1996-01-01 00:00:00
abstract::The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029010
更新日期:1993-10-01 00:00:00
abstract:KEY MESSAGE:AFP improved cryopreservation efficiency of potato (Solanum tuberosum cv. Superior) by regulating transcript levels of CBF1 and DHN1. However, the optimal AFP concentration required for strong induction of the genes was dependent on the type of vitrification solution to which the AFP was added: This finding...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0743-8
更新日期:2018-07-01 00:00:00
abstract::The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005867215258
更新日期:1997-05-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract::Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9399-0
更新日期:2008-12-01 00:00:00
abstract::The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015650
更新日期:1990-01-01 00:00:00
abstract::With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000036368.74758.66
更新日期:2004-02-01 00:00:00
abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028864
更新日期:1994-11-01 00:00:00
abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-7307-4
更新日期:2005-07-01 00:00:00
abstract::Tandemly arrayed genes (TAGs) account for about one-third of the duplicated genes in eukaryotic genomes. They provide raw genetic material for biological evolution, and play important roles in genome evolution. The 22-kDa prolamin genes in cereal genomes represent typical TAG organization, and provide the good materia...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9705-5
更新日期:2010-12-01 00:00:00
abstract::Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005729309569
更新日期:1997-03-01 00:00:00
abstract::Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-1303-y
更新日期:2004-07-01 00:00:00
abstract::Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9414-5
更新日期:2009-01-01 00:00:00
abstract::Rice storage protein glutelin genes are coordinately regulated during seed development. A previous 5' deletion analysis using transgenic tobacco revealed that the minimum 5' region necessary for endosperm specificity was within -245 bp of the transcription start site, and included the AACA and GCN4 motifs that are hig...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026459229671
更新日期:1999-05-01 00:00:00
abstract::Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a su...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0527-y
更新日期:2016-11-01 00:00:00