DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli.

abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...

journal_title：Plant molecular biology

authors： Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GW

更新日期：2013-01-01 00:00:00

abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...

journal_title：Plant molecular biology

authors： Kohno-Murase J,Murase M,Ichikawa H,Imamura J

更新日期：1994-11-01 00:00:00

abstract：:The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...

journal_title：Plant molecular biology

authors： Itoh R,Takano H,Ohta N,Miyagishima S,Kuroiwa H,Kuroiwa T

更新日期：1999-10-01 00:00:00

abstract：:We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

journal_title：Plant molecular biology

authors： Carballo M,Giné R,Santos M,Puigdomènech P

更新日期：1991-01-01 00:00:00

abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

journal_title：Plant molecular biology

authors： Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

更新日期：1999-07-01 00:00:00

abstract：:Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...

journal_title：Plant molecular biology

authors： Qiu LJ,Xing LL,Guo Y,Wang J,Jackson SA,Chang RZ

更新日期：2013-09-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a su...

journal_title：Plant molecular biology

authors： Jung JH,Kannan B,Dermawan H,Moxley GW,Altpeter F

更新日期：2016-11-01 00:00:00

abstract：:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

journal_title：Plant molecular biology

authors： Turek I,Gehring C

更新日期：2016-06-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...

journal_title：Plant molecular biology

authors： Mock HP,Trainotti L,Kruse E,Grimm B

更新日期：1995-05-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

journal_title：Plant molecular biology

authors： Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

更新日期：2006-08-01 00:00:00

abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...

journal_title：Plant molecular biology

authors： Rawat R,Xu ZF,Yao KM,Chye ML

更新日期：2005-03-01 00:00:00

abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...

journal_title：Plant molecular biology

authors： Putnam-Evans C,Wu J,Bricker TM

更新日期：1996-12-01 00:00:00

abstract：:Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...

journal_title：Plant molecular biology

authors： Bassani M,Neumann PM,Gepstein S

更新日期：2004-10-01 00:00:00

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...

journal_title：Plant molecular biology

authors： Li ZM,Zhang JZ,Mei L,Deng XX,Hu CG,Yao JL

更新日期：2010-09-01 00:00:00

abstract：:Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...

journal_title：Plant molecular biology

authors： Guo D,Gao X,Li H,Zhang T,Chen G,Huang P,An L,Li N

更新日期：2008-03-01 00:00:00

abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...

journal_title：Plant molecular biology

authors： Diaz EC,Martin F,Tagu D

更新日期：1996-07-01 00:00:00

abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

journal_title：Plant molecular biology

authors： Riccardi G,De Rossi E,Della Valle G,Ciferri O

更新日期：1985-03-01 00:00:00

abstract：:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three dis...

journal_title：Plant molecular biology

authors： Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

更新日期：1992-11-01 00:00:00

abstract：:This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

journal_title：Plant molecular biology

authors： Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

更新日期：1998-12-01 00:00:00

abstract：:The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...

journal_title：Plant molecular biology

authors： Garcia ME,Lynch T,Peeters J,Snowden C,Finkelstein R

更新日期：2008-08-01 00:00:00

abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

journal_title：Plant molecular biology

authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

更新日期：1992-01-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...

journal_title：Plant molecular biology

authors： van Engelen FA,de Jong AJ,Meijer EA,Kuil CW,Meyboom JK,Dirkse WG,Booij H,Hartog MV,Vandekerckhove J,de Vries SC

更新日期：1995-03-01 00:00:00

abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...

journal_title：Plant molecular biology

authors： Latvala-Kilby SM,Kilby NJ

更新日期：2006-05-01 00:00:00

abstract：:A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....

journal_title：Plant molecular biology

authors： Luo H,Lee JY,Hu Q,Nelson-Vasilchik K,Eitas TK,Lickwar C,Kausch AP,Chandlee JM,Hodges TK

更新日期：2006-10-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...

journal_title：Plant molecular biology

authors： Raj-Kumar PK,Vallon O,Liang C

更新日期：2017-06-01 00:00:00