Abstract：
:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin promoter. Prediction based deletion analysis of both the promoter elements confirmed the necessary cis-acting regulatory elements as well as the minimal promoter element for optimal expression in each case. The results also revealed that cis-regulatory elements might have been responsible for high level expression as well as spatio-temporal regulation of the sesame 2S albumin promoter. Transgenic over-expression of a fatty acid desaturase (fad3C) gene of soybean driven by 2S albumin promoter resulted in seed-specific enhanced level of α-linolenic acid in sesame. The present study, for the first time helped to identify that the sesame 2S albumin promoter is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal regulation of gene(s) of interest in sesame and can also be useful as a heterologous genetic element in other important oil seed crop plants in general for which seed oil is the harvested product. The study also established the feasibility of fatty acid metabolic engineering strategy undertaken to improve quality of edible seed oil in sesame using the 2S albumin promoter as regulatory element.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SKdoi
10.1007/s11103-014-0233-6subject
Has Abstractpub_date
2014-11-01 00:00:00pages
351-65issue
4-5eissn
0167-4412issn
1573-5028journal_volume
86pub_type
杂志文章abstract：KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0677-6
更新日期：2017-12-01 00:00:00
abstract：:The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023564
更新日期：1994-02-01 00:00:00
abstract：:Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1011628024798
更新日期：2001-10-01 00:00:00
abstract：:Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9784-y
更新日期：2011-07-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005889314967
更新日期：1997-05-01 00:00:00
abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418241
更新日期：1985-07-01 00:00:00
abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015034
更新日期：1987-05-01 00:00:00
abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01018-7
更新日期：2020-08-01 00:00:00
abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9785-x
更新日期：2011-08-01 00:00:00
abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017450
更新日期：1989-01-01 00:00:00
abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1023937006311
更新日期：2003-05-01 00:00:00
abstract：:We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006196530079
更新日期：1999-05-01 00:00:00
abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014935
更新日期：1993-05-01 00:00:00
abstract：KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01071-2
更新日期：2021-01-01 00:00:00
abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0954-7
更新日期：2005-03-01 00:00:00
abstract：:Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9448-8
更新日期：2009-04-01 00:00:00
abstract：:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039627
更新日期：1993-01-01 00:00:00
abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019506
更新日期：1994-12-01 00:00:00
abstract：:The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9344-2
更新日期：2008-08-01 00:00:00
abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040527
更新日期：1992-09-01 00:00:00
abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006499832424
更新日期：2000-06-01 00:00:00
abstract：:Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9322-8
更新日期：2008-07-01 00:00:00
abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9283-3
更新日期：2008-03-01 00:00:00
abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010686931889
更新日期：2001-07-01 00:00:00
abstract：:Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039544
更新日期：1994-10-01 00:00:00
abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9441-2
更新日期：2009-03-01 00:00:00
abstract：:Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014876409166
更新日期：2002-03-01 00:00:00
abstract：:Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00043663
更新日期：1995-10-01 00:00:00
