Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato.

Abstract:

:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene consisting of 1015 bp of 5'-flanking sequence and part of the first exon of STH-2 fused to the bacterial beta-glucuronidase gene was highly-expressed in tubers of transgenic potato plants after wounding and elicitor treatments. The levels of activity observed in these transgenic plants parallel those observed for the accumulation of STH-2 mRNAs under similar conditions. This indicates that cis-acting elements necessary for the proper activation of the gene are present within 1 kb of 5'-flanking sequences. Functional analysis of 5' deletions of the STH-2/GUS constructs by transient expression in leaf protoplasts revealed the presence of an upstream regulatory sequence between -135 and -52 which contains a TGAC motif, and a possible negative regulatory region between -52 and -28. A factor present in nuclear extracts of wounded potato tubers was found to bind specifically to nucleotides located between -135 to -105, suggesting that this region contains important cis-regulatory elements.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

doi

10.1007/BF00014935

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

279-91

issue

2

eissn

0167-4412

issn

1573-5028

journal_volume

22

pub_type

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