Expression of lorelei-like genes in aposporous and sexual Paspalum notatum plants.

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...

journal_title：Plant molecular biology

authors： Breton C,Chaboud A,Matthys-Rochon E,Bates EE,Cock JM,Fromm H,Dumas C

更新日期：1995-01-01 00:00:00

abstract：:Ribose-5-phosphate isomerase (RPI) catalyses the interconversion of ribose-5-phosphate and ribulose-5-phosphate in the reductive and oxidative pentose phosphate pathways in plants. RPI from spinach chloroplasts was purified and microsequenced. Via PCR with degenerate primers designed against microsequenced peptides, a...

journal_title：Plant molecular biology

authors： Martin W,Henze K,Kellerman J,Flechner A,Schnarrenberger C

更新日期：1996-02-01 00:00:00

abstract：:The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...

journal_title：Plant molecular biology

authors： Ogita S,Uefuji H,Morimoto M,Sano H

更新日期：2004-04-01 00:00:00

abstract：:We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...

journal_title：Plant molecular biology

authors： Kurkela S,Franck M

更新日期：1990-07-01 00:00:00

abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...

journal_title：Plant molecular biology

authors： Scott RJ,Draper J

更新日期：1987-05-01 00:00:00

abstract：:The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant...

journal_title：Plant molecular biology

authors： Malinowski R,Higgins R,Luo Y,Piper L,Nazir A,Bajwa VS,Clouse SD,Thompson PR,Stratmann JW

更新日期：2009-07-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesi...

journal_title：Plant molecular biology

authors： Hansen G,Tempé J,Brevet J

更新日期：1992-10-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...

journal_title：Plant molecular biology

authors： Muchhal US,Schwartzbach SD

更新日期：1992-01-01 00:00:00

abstract：:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

journal_title：Plant molecular biology

authors： Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

更新日期：2007-02-01 00:00:00

abstract：:We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...

journal_title：Plant molecular biology

authors： van Doorn WG,Balk PA,van Houwelingen AM,Hoeberichts FA,Hall RD,Vorst O,van der Schoot C,van Wordragen MF

更新日期：2003-12-01 00:00:00

abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

journal_title：Plant molecular biology

authors： Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

更新日期：2005-02-01 00:00:00

abstract：:We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and i...

journal_title：Plant molecular biology

authors： Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein H

更新日期：1994-10-01 00:00:00

abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

journal_title：Plant molecular biology

authors： Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

更新日期：1994-01-01 00:00:00

abstract：KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...

journal_title：Plant molecular biology

authors： Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

更新日期：2018-02-01 00:00:00

abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...

journal_title：Plant molecular biology

authors： Xu H,Wang N,Liu J,Qu C,Wang Y,Jiang S,Lu N,Wang D,Zhang Z,Chen X

更新日期：2017-05-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

journal_title：Plant molecular biology

authors： Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

更新日期：2019-09-01 00:00:00

abstract：:The constituents of plasma membrane proteins, particularly the integral membrane proteins, are closely associated with the differentiation of plant cells. Secondary vascular differentiation, which gives rise to the increase in plant stem diameter, is the key process by which the volume of the plant body grows. However...

journal_title：Plant molecular biology

authors： Song D,Xi W,Shen J,Bi T,Li L

更新日期：2011-05-01 00:00:00

abstract：KEY MESSAGE:We identified three dormant stages of Polygonatum kingianum and changes that occurred during dormancy transition in the following aspects including cell wall and hormones, as well as interaction among them. Polygonatum kingianum Coll.et Hemsl (P. kingianum) is an important traditional Chinese medicine, but ...

journal_title：Plant molecular biology

authors： Wang Y,Liu X,Su H,Yin S,Han C,Hao D,Dong X

更新日期：2019-02-01 00:00:00

abstract：:Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...

journal_title：Plant molecular biology

authors： Takaiwa F,Oono K,Wing D,Kato A

更新日期：1991-10-01 00:00:00

abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...

journal_title：Plant molecular biology

authors： Bennetzen JL

更新日期：2000-01-01 00:00:00

abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

journal_title：Plant molecular biology

authors： Sohal AK,Pallas JA,Jenkins GI

更新日期：1999-09-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...

journal_title：Plant molecular biology

authors： Pareek A,Singla SL,Grover A

更新日期：1995-10-01 00:00:00

abstract：KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

journal_title：Plant molecular biology

authors： Geilfus CM,Lan J,Carpentier S

更新日期：2018-12-01 00:00:00

abstract：:Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...

journal_title：Plant molecular biology

authors： DeRocher A,Vierling E

更新日期：1995-02-01 00:00:00