PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis.

abstract：:Dobzhansky stated that nothing in biology makes sense except in the light of evolution. A close corollary, and the central theme of this paper, is that everything makes a lot more sense in the light of phylogeny. Systematics is in the midst of a renaissance, heralded by the widespread application of new analytical app...

journal_title：Plant molecular biology

authors： Soltis ED,Soltis PS

更新日期：2000-01-01 00:00:00

abstract：:During the course of characterizing polymerase chain reaction products corresponding to protein kinases of a higher plant, Arabidopsis thaliana, we found a DNA fragment that potentially codes for a polypeptide with mosaic sequences of two classes of protein kinases, a tyrosine-specific and a serine/threonine-specific ...

journal_title：Plant molecular biology

authors： Hirayama T,Oka A

更新日期：1992-11-01 00:00:00

abstract：:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...

journal_title：Plant molecular biology

authors： Sonti RV,Tissier AF,Wong D,Viret JF,Signer ER

更新日期：1995-09-01 00:00:00

abstract：:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...

journal_title：Plant molecular biology

authors： Henrissat B,Coutinho PM,Davies GJ

更新日期：2001-09-01 00:00:00

abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....

journal_title：Plant molecular biology

authors： De Buck S,Van Montagu M,Depicker A

更新日期：2001-07-01 00:00:00

abstract：:Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...

journal_title：Plant molecular biology

authors： Botella JR,Arteca RN

更新日期：1994-03-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...

journal_title：Plant molecular biology

authors： Menz MA,Klein RR,Mullet JE,Obert JA,Unruh NC,Klein PE

更新日期：2002-03-01 00:00:00

abstract：:A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...

journal_title：Plant molecular biology

authors： Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama K

更新日期：2003-09-01 00:00:00

abstract：:In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

journal_title：Plant molecular biology

authors： van Tunen AJ,Hartman SA,Mur LA,Mol JN

更新日期：1989-05-01 00:00:00

abstract：:This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

journal_title：Plant molecular biology

authors： Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

更新日期：1998-12-01 00:00:00

abstract：:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

journal_title：Plant molecular biology

authors： van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

更新日期：1994-10-01 00:00:00

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

journal_title：Plant molecular biology

authors： Lertpiriyapong K,Sung ZR

更新日期：2003-11-01 00:00:00

abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

journal_title：Plant molecular biology

authors： Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

更新日期：2009-05-01 00:00:00

abstract：:In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

journal_title：Plant molecular biology

authors： Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

更新日期：2015-06-01 00:00:00

abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...

journal_title：Plant molecular biology

authors： Yao H,Kato A,Mooney B,Birchler JA

更新日期：2011-02-01 00:00:00

abstract：:The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...

journal_title：Plant molecular biology

authors： Pogson BJ,Davies C

更新日期：1995-05-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00

abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...

journal_title：Plant molecular biology

authors： Kohno-Murase J,Murase M,Ichikawa H,Imamura J

更新日期：1994-11-01 00:00:00

abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...

journal_title：Plant molecular biology

authors： Cinege G,Louis S,Hänsch R,Schnitzler JP

更新日期：2009-03-01 00:00:00

abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...

journal_title：Plant molecular biology

authors： Jensen MK,Rung JH,Gregersen PL,Gjetting T,Fuglsang AT,Hansen M,Joehnk N,Lyngkjaer MF,Collinge DB

更新日期：2007-09-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

journal_title：Plant molecular biology

authors： Romano CP,Robson PR,Smith H,Estelle M,Klee H

更新日期：1995-03-01 00:00:00

abstract：:To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...

journal_title：Plant molecular biology

authors： Nanjo Y,Maruyama K,Yasue H,Yamaguchi-Shinozaki K,Shinozaki K,Komatsu S

更新日期：2011-09-01 00:00:00

abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...

journal_title：Plant molecular biology

authors： Junker BH,Wuttke R,Tiessen A,Geigenberger P,Sonnewald U,Willmitzer L,Fernie AR

更新日期：2004-09-01 00:00:00

abstract：:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...

journal_title：Plant molecular biology

authors： Hübschmann T,Börner T

更新日期：1998-02-01 00:00:00

abstract：:Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset ...

journal_title：Plant molecular biology

authors： Liu L,Zhou Y,Zhou G,Ye R,Zhao L,Li X,Lin Y

更新日期：2008-05-01 00:00:00

abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

journal_title：Plant molecular biology

authors： van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

更新日期：1989-11-01 00:00:00