Activity of the yeast FLP recombinase in Arabidopsis.

Abstract:

:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Sonti RV,Tissier AF,Wong D,Viret JF,Signer ER

doi

10.1007/BF00032673

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

1127-32

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

28

pub_type

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  • Diversity in the complexity of phosphate starvation transcriptomes among rice cultivars based on RNA-Seq profiles.

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