Abstract:
:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Sonti RV,Tissier AF,Wong D,Viret JF,Signer ERdoi
10.1007/BF00032673subject
Has Abstractpub_date
1995-09-01 00:00:00pages
1127-32issue
6eissn
0167-4412issn
1573-5028journal_volume
28pub_type
杂志文章abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
abstract::A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9660-1
更新日期:2010-09-01 00:00:00
abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043874
更新日期:1994-06-01 00:00:00
abstract::Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043663
更新日期:1995-10-01 00:00:00
abstract::Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019477
更新日期:1996-07-01 00:00:00
abstract::Darjeeling teas are the highest grown teas in the world and preferred for its flavour, aroma and quality. Apart from the genetic makeup of the plant, earlier reports suggest that insect infestation, particularly jassids and thrips triggers the aroma and flavour formation in Darjeeling tea. The present work encompasses...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9887-0
更新日期:2012-04-01 00:00:00
abstract::Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0082-8
更新日期:2013-10-01 00:00:00
abstract::Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genet...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0530-3
更新日期:2016-11-01 00:00:00
abstract::Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006498413543
更新日期:2000-09-01 00:00:00
abstract:KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-018-0771-4
更新日期:2018-09-01 00:00:00
abstract::Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0430-6
更新日期:2016-04-01 00:00:00
abstract:KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0684-7
更新日期:2018-02-01 00:00:00
abstract::A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0161-x
更新日期:2014-04-01 00:00:00
abstract::A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016504
更新日期:1990-05-01 00:00:00
abstract::Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0106-4
更新日期:2013-12-01 00:00:00
abstract::Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9099-6
更新日期:2007-02-01 00:00:00
abstract::cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041154
更新日期:1995-11-01 00:00:00
abstract::DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039524
更新日期:1994-10-01 00:00:00
abstract::A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005830313272
更新日期:1997-10-01 00:00:00
abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418241
更新日期:1985-07-01 00:00:00
abstract::The constituents of plasma membrane proteins, particularly the integral membrane proteins, are closely associated with the differentiation of plant cells. Secondary vascular differentiation, which gives rise to the increase in plant stem diameter, is the key process by which the volume of the plant body grows. However...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9771-3
更新日期:2011-05-01 00:00:00
abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014183
更新日期:1987-01-01 00:00:00
abstract::The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020496
更新日期:1996-12-01 00:00:00
abstract::Secretion, endocytosis and transport to the lytic compartment are fundamental, highly coordinated features of the eukaryotic cell. These intracellular transport processes are facilitated by vesicles, many of which are small (100 nm or less in diameter) and 'coated' on their cytoplasmic surface. Research into the struc...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1998-09-01 00:00:00
abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016123
更新日期:1990-11-01 00:00:00
abstract::Many studies in both animal and plant systems have shown that matrix attachment regions (MARs) can increase the expression of flanking transgenes. However, our previous studies revealed no effect of the chicken lysozyme MARs (chiMARs) on transgene expression in the first generation transgenic Arabidopsis thaliana plan...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9107-x
更新日期:2007-03-01 00:00:00
abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027342
更新日期:1992-05-01 00:00:00
abstract::The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023992
更新日期:1991-03-01 00:00:00
abstract::In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019952
更新日期:1993-01-01 00:00:00