The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants.

abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

journal_title：Plant molecular biology

authors： Ringli C,Keller B

更新日期：1998-08-01 00:00:00

abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...

journal_title：Plant molecular biology

authors： Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BS

更新日期：2016-06-01 00:00:00

abstract：:Plant microRNAs (miRNAs) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation. A number of miRNAs play important roles in development. In order to investigate the potential function of miRNAs during male gametogenesis in rice, we obtained both gene and small RNA express...

journal_title：Plant molecular biology

authors： Peng H,Chun J,Ai TB,Tong YA,Zhang R,Zhao MM,Chen F,Wang SH

更新日期：2012-09-01 00:00:00

abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

journal_title：Plant molecular biology

authors： Rose AB,Carter A,Korf I,Kojima N

更新日期：2016-10-01 00:00:00

abstract：:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...

journal_title：Plant molecular biology

authors： Ma DP,King YT,Kim Y,Luckett WS Jr

更新日期：1992-03-01 00:00:00

abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...

journal_title：Plant molecular biology

authors： Cervantes E,Rodríguez A,Nicolás G

更新日期：1994-05-01 00:00:00

abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

journal_title：Plant molecular biology

authors： Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

更新日期：1992-02-01 00:00:00

abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...

journal_title：Plant molecular biology

authors： Laxa M

更新日期：2017-01-01 00:00:00

abstract：:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

journal_title：Plant molecular biology

authors： Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

更新日期：2002-05-01 00:00:00

abstract：:Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue...

journal_title：Plant molecular biology

authors： van der Steege G,Nieboer M,Swaving J,Tempelaar MJ

更新日期：1992-10-01 00:00:00

abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...

journal_title：Plant molecular biology

authors： Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler F

更新日期：1993-11-01 00:00:00

abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

journal_title：Plant molecular biology

authors： Sohal AK,Pallas JA,Jenkins GI

更新日期：1999-09-01 00:00:00

abstract：:The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

journal_title：Plant molecular biology

authors： Grimmig B,Matern U

更新日期：1997-01-01 00:00:00

abstract：:A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-termin...

journal_title：Plant molecular biology

authors： Corr-Menguy F,Cejudo FJ,Mazubert C,Vidal J,Lelandais-Brière C,Torres G,Rode A,Hartmann C

更新日期：2002-11-01 00:00:00

abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...

journal_title：Plant molecular biology

authors： Albani D,Parisi B,Carbonera D,Cella R

更新日期：1985-11-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：:RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

journal_title：Plant molecular biology

authors： Petersen BO,Albrechtsen M

更新日期：2005-07-01 00:00:00

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...

journal_title：Plant molecular biology

authors： Luque I,Flores E,Herrero A

更新日期：1993-03-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...

journal_title：Plant molecular biology

authors： Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

更新日期：2018-02-01 00:00:00

abstract：:Due to an unfortunate turn of events, the funding note for Open Access publication was not properly provided in the original publication. Hence, the original article has been corrected. The opening line of the Acknowledgement section should read. ...

journal_title：Plant molecular biology

authors： Luck K,Jia Q,Huber M,Handrick V,Wong GK,Nelson DR,Chen F,Gershenzon J,Köllner TG

更新日期：2017-12-01 00:00:00

abstract：:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

journal_title：Plant molecular biology

authors： Morris BA,Richardson KA,Andersen MT,Gardner RC

更新日期：1988-11-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

journal_title：Plant molecular biology

authors： Leah R,Mundy J

更新日期：1989-06-01 00:00:00

abstract：:We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

journal_title：Plant molecular biology

authors： Makaroff CA,Apel IJ,Palmer JD

更新日期：1990-11-01 00:00:00

abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...

journal_title：Plant molecular biology

authors： Zhang Y,Schwarz S,Saedler H,Huijser P

更新日期：2007-02-01 00:00:00

abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...

journal_title：Plant molecular biology

authors： Maiti MK,Krishnasamy S,Owen HA,Makaroff CA

更新日期：1997-11-01 00:00:00

abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

journal_title：Plant molecular biology

authors： Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

更新日期：2004-05-01 00:00:00

abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

journal_title：Plant molecular biology

authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

更新日期：2009-02-01 00:00:00

abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...

journal_title：Plant molecular biology

authors： Hu J,Reddy VS,Wessler SR

更新日期：2000-03-01 00:00:00