Abstract:
:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate restriction endonuclease targets have also been placed between the border regions. These binary vectors, in conjunction with appropriate Agrobacterium strains, are capable of delivering DNA to plant cells in cocultivation experiments with very high efficiency. The transformation frequency is shown to be somewhat dependent on the replicon used. re]19850121 rv]19850506 ac]19850513.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
van den Elzen P,Lee KY,Townsend J,Bedbrook Jdoi
10.1007/BF00015678subject
Has Abstractpub_date
1985-05-01 00:00:00pages
149-54issue
3eissn
0167-4412issn
1573-5028journal_volume
5pub_type
杂志文章abstract::As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024201
更新日期:1994-04-01 00:00:00
abstract::We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027072
更新日期:1992-08-01 00:00:00
abstract::The formation and the development of the floral organs require an intercalate expression of organ-specific genes. At the same time, meristem-specific genes are repressed to complete the differentiation of the organs in the floral whorls. In an Arabidopsis activation tagging population, a mutant affected in inflorescen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-0698-4
更新日期:2005-03-01 00:00:00
abstract:KEY MESSAGE:Two OsDGAT1 genes showed the ability to restore TAG and LB synthesis in yeast H1246. Alterations in the N-terminal region of OsDGAT1-1 gene revealed its regulatory role in gene function. Accumulation of triacylglycerol (TAG) or oil in vegetative tissues has emerged as a promising approach to meet the global...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01085-w
更新日期:2020-10-21 00:00:00
abstract::A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020388
更新日期:1995-06-01 00:00:00
abstract::We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014440531842
更新日期:2002-05-01 00:00:00
abstract::The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037557
更新日期:1985-11-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00044158
更新日期:1989-06-01 00:00:00
abstract::The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00019401
更新日期:1990-06-01 00:00:00
abstract::Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9779-8
更新日期:2011-05-01 00:00:00
abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375123496
更新日期:1999-11-01 00:00:00
abstract::Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040638
更新日期:1991-09-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract::We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005916821224
更新日期:1998-03-01 00:00:00
abstract:KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0563-7
更新日期:2017-02-01 00:00:00
abstract::A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005728411921
更新日期:1997-02-01 00:00:00
abstract::RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0069-5
更新日期:2013-07-01 00:00:00
abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9428-z
更新日期:2009-02-01 00:00:00
abstract::To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
abstract::Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2619-y
更新日期:2005-12-01 00:00:00
abstract::A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021542
更新日期:1993-11-01 00:00:00
abstract:KEY MESSAGE:The induction of miR399 and miR398 and the inhibition of miR156, miR159, miR160, miR171, miR2111, and miR2643 were observed under Pi deficiency in alfalfa. The miRNA-mediated genes involved in basic metabolic process, root and shoot development, stress response and Pi uptake. Inorganic phosphate (Pi) defici...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0711-3
更新日期:2018-03-01 00:00:00
abstract::We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017731
更新日期:1990-07-01 00:00:00
abstract:KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01045-4
更新日期:2020-10-01 00:00:00
abstract::Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-06-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00
abstract::DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020892
更新日期:1995-03-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00
abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023564
更新日期:1994-02-01 00:00:00
abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005909703600
更新日期:1998-05-01 00:00:00