Simple binary vectors for DNA transfer to plant cells.

Abstract:

:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate restriction endonuclease targets have also been placed between the border regions. These binary vectors, in conjunction with appropriate Agrobacterium strains, are capable of delivering DNA to plant cells in cocultivation experiments with very high efficiency. The transformation frequency is shown to be somewhat dependent on the replicon used. re]19850121 rv]19850506 ac]19850513.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

van den Elzen P,Lee KY,Townsend J,Bedbrook J

doi

10.1007/BF00015678

subject

Has Abstract

pub_date

1985-05-01 00:00:00

pages

149-54

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

5

pub_type

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