Abstract:
:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler Fdoi
10.1007/BF00021542subject
Has Abstractpub_date
1993-11-01 00:00:00pages
881-8issue
4eissn
0167-4412issn
1573-5028journal_volume
23pub_type
杂志文章abstract::Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9655-y
更新日期:2010-09-01 00:00:00
abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019126
更新日期:1995-10-01 00:00:00
abstract::Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0173-1
更新日期:2014-05-01 00:00:00
abstract::We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016067200148
更新日期:2002-09-01 00:00:00
abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01071-2
更新日期:2021-01-01 00:00:00
abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005708611020
更新日期:1997-04-01 00:00:00
abstract::We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3249-5
更新日期:2004-09-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00044158
更新日期:1989-06-01 00:00:00
abstract::The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021266320877
更新日期:2002-12-01 00:00:00
abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3474-y
更新日期:2004-10-01 00:00:00
abstract::A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005704616565
更新日期:1997-03-01 00:00:00
abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000009269.97773.70
更新日期:2003-09-01 00:00:00
abstract::The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8883-z
更新日期:2005-09-01 00:00:00
abstract::An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040577
更新日期:1994-01-01 00:00:00
abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028878
更新日期:1994-08-01 00:00:00
abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019191
更新日期:1995-01-01 00:00:00
abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-005-7703-9
更新日期:2006-04-01 00:00:00
abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039544
更新日期:1994-10-01 00:00:00
abstract::Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006138221874
更新日期:1999-03-01 00:00:00
abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5817-8
更新日期:2006-05-01 00:00:00
abstract::Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0501-y
更新日期:2004-05-01 00:00:00
abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020464
更新日期:1995-12-01 00:00:00
abstract::Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9456-3
更新日期:2009-05-01 00:00:00
abstract::A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020982
更新日期:1995-11-01 00:00:00
abstract::Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023971315108
更新日期:2003-05-01 00:00:00
abstract::When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020560
更新日期:1991-02-01 00:00:00
abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016071
更新日期:1991-06-01 00:00:00
abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-010-9599-2
更新日期:2010-05-01 00:00:00