Cloning, sequence analysis and expression of a cDNA encoding active phosphoenolpyruvate carboxylase of the C3 plant Solanum tuberosum.

Abstract:

:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler F

doi

10.1007/BF00021542

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

881-8

issue

4

eissn

0167-4412

issn

1573-5028

journal_volume

23

pub_type

杂志文章
  • Two O-methyltransferases involved in the biosynthesis of methoxypyrazines: grape-derived aroma compounds important to wine flavour.

    abstract::Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9655-y

    authors: Dunlevy JD,Soole KL,Perkins MV,Dennis EG,Keyzers RA,Kalua CM,Boss PK

    更新日期:2010-09-01 00:00:00

  • Field trial analysis of nitrate reductase co-suppression: a comparative study of 38 combinations of transgene loci.

    abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019126

    authors: Palauqui JC,Vaucheret H

    更新日期:1995-10-01 00:00:00

  • Pigmentation in sand pear (Pyrus pyrifolia) fruit: biochemical characterization, gene discovery and expression analysis with exocarp pigmentation mutant.

    abstract::Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0173-1

    authors: Wang YZ,Zhang S,Dai MS,Shi ZB

    更新日期:2014-05-01 00:00:00

  • Tobacco-expressed Brassica juncea chitinase BjCHI1 shows antifungal activity in vitro.

    abstract::We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1016067200148

    authors: Fung KL,Zhao KJ,He ZM,Chye ML

    更新日期:2002-09-01 00:00:00

  • Rice-specific Argonaute 17 controls reproductive growth and yield-associated phenotypes.

    abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01071-2

    authors: Pachamuthu K,Swetha C,Basu D,Das S,Singh I,Sundar VH,Sujith TN,Shivaprasad PV

    更新日期:2021-01-01 00:00:00

  • Molecular cloning, structure and expression of an elicitor-inducible chitinase gene from pine trees.

    abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005708611020

    authors: Wu H,Echt CS,Popp MP,Davis JM

    更新日期:1997-04-01 00:00:00

  • Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces.

    abstract::We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3249-5

    authors: Fujimoto S,Matsunaga S,Yonemura M,Uchiyama S,Azuma T,Fukui K

    更新日期:2004-09-01 00:00:00

  • The bifunctional α-amylase/subtilisin inhibitor of barley: nucleotide sequence and patterns of seed-specific expression.

    abstract::We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00044158

    authors: Leah R,Mundy J

    更新日期:1989-06-01 00:00:00

  • Protein-protein interactions in the secretory pathway, a growing demand for experimental approaches in vivo.

    abstract::The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1021266320877

    authors: Pimpl P,Denecke J

    更新日期:2002-12-01 00:00:00

  • A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis.

    abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0249-y

    authors: Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

    更新日期:2014-12-01 00:00:00

  • Differential expression profiles of growth-related genes in the elongation zone of maize primary roots.

    abstract::Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3474-y

    authors: Bassani M,Neumann PM,Gepstein S

    更新日期:2004-10-01 00:00:00

  • Curculin, a sweet-tasting and taste-modifying protein, is a non-functional mannose-binding lectin.

    abstract::A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005704616565

    authors: Barre A,Van Damme EJ,Peumans WJ,Rougé P

    更新日期:1997-03-01 00:00:00

  • A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine.

    abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000009269.97773.70

    authors: Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama K

    更新日期:2003-09-01 00:00:00

  • Genomic analysis of the 12-oxo-phytodienoic acid reductase gene family of Zea mays.

    abstract::The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-8883-z

    authors: Zhang J,Simmons C,Yalpani N,Crane V,Wilkinson H,Kolomiets M

    更新日期:2005-09-01 00:00:00

  • The highly expressed tapetum-specific A9 gene is not required for male fertility in Brassica napus.

    abstract::An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040577

    authors: Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

    更新日期:1994-01-01 00:00:00

  • Cyclophilins are encoded by a small gene family in rice.

    abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028878

    authors: Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

    更新日期:1994-08-01 00:00:00

  • Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

    abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019191

    authors: Packer JC,André D,Howe CJ

    更新日期:1995-01-01 00:00:00

  • Leaf initiation: the integration of growth and cell division.

    abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-005-7703-9

    authors: Fleming AJ

    更新日期:2006-04-01 00:00:00

  • Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice.

    abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039544

    authors: Takahashi R,Joshee N,Kitagawa Y

    更新日期:1994-10-01 00:00:00

  • Transposable element contributions to plant gene and genome evolution.

    abstract::Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:

    authors: Bennetzen JL

    更新日期:2000-01-01 00:00:00

  • GRAB proteins, novel members of the NAC domain family, isolated by their interaction with a geminivirus protein.

    abstract::Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006138221874

    authors: Xie Q,Sanz-Burgos AP,Guo H,García JA,Gutiérrez C

    更新日期:1999-03-01 00:00:00

  • Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants.

    abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-5817-8

    authors: Ueda H,Yamaguchi Y,Sano H

    更新日期:2006-05-01 00:00:00

  • Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope.

    abstract::Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-0501-y

    authors: Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

    更新日期:2004-05-01 00:00:00

  • Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

    abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020464

    authors: Espartero J,Sánchez-Aguayo I,Pardo JM

    更新日期:1995-12-01 00:00:00

  • Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.

    abstract::Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9456-3

    authors: Huo N,Vogel JP,Lazo GR,You FM,Ma Y,McMahon S,Dvorak J,Anderson OD,Luo MC,Gu YQ

    更新日期:2009-05-01 00:00:00

  • Functional analysis of a recently originating, atypical presequence: mitochondrial import and processing of GUS fusion proteins in transgenic tobacco and yeast.

    abstract::A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020982

    authors: Huang J,Levings CS 3rd

    更新日期:1995-11-01 00:00:00

  • Molecular characterization of His-Asp phosphorelay signaling factors in maize leaves: implications of the signal divergence by cytokinin-inducible response regulators in the cytosol and the nuclei.

    abstract::Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1023971315108

    authors: Asakura Y,Hagino T,Ohta Y,Aoki K,Yonekura-Sakakibara K,Deji A,Yamaya T,Sugiyama T,Sakakibara H

    更新日期:2003-05-01 00:00:00

  • Blue light is required for survival of the tomato phytochrome-deficient aurea mutant and the expression of four nuclear genes coding for plastidic proteins.

    abstract::When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020560

    authors: Oelmüller R,Kendrick RE

    更新日期:1991-02-01 00:00:00

  • Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression.

    abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016071

    authors: van der Zaal EJ,Droog FN,Boot CJ,Hensgens LA,Hoge JH,Schilperoort RA,Libbenga KR

    更新日期:1991-06-01 00:00:00

  • Axillary bud outgrowth in herbaceous shoots: how do strigolactones fit into the picture?

    abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-010-9599-2

    authors: Waldie T,Hayward A,Beveridge CA

    更新日期:2010-05-01 00:00:00