Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

Abstract:

:Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic cyanobacterium Phormidium laminosum which is believed to encode an enzyme common to both membrane systems. The deduced amino acid sequence is 203 residues long and although the overall similarity among signal peptidases is rather low there are a number of identifiable conserved regions present. The P. laminosum enzyme is predicted to have a single transmembrane domain, in contrast to other Gram-negative bacterial sequences, but similar to other type I signal peptidases.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Packer JC,André D,Howe CJ

doi

10.1007/BF00019191

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

199-204

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

27

pub_type

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