Molecular characterization of His-Asp phosphorelay signaling factors in maize leaves: implications of the signal divergence by cytokinin-inducible response regulators in the cytosol and the nuclei.

abstract：:In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...

journal_title：Plant molecular biology

authors： Dowson Day MJ,Ashurst JL,Mathias SF,Watts JW,Wilson TM,Dixon RA

更新日期：1993-10-01 00:00:00

abstract：:Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...

journal_title：Plant molecular biology

authors： Vogel H,Fischer S,Valentin K

更新日期：1996-11-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...

journal_title：Plant molecular biology

authors： Oelmüller R,Kendrick RE

更新日期：1991-02-01 00:00:00

abstract：:We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...

journal_title：Plant molecular biology

authors： Köhler U,Cerff R,Brinkmann H

更新日期：1996-01-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...

journal_title：Plant molecular biology

authors： Lin F,Jiang L,Liu Y,Lv Y,Dai H,Zhao H

更新日期：2014-11-01 00:00:00

abstract：:Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...

journal_title：Plant molecular biology

authors： Yang P,Taoka K,Nakayama T,Iwabuchi M

更新日期：1995-04-01 00:00:00

abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

journal_title：Plant molecular biology

authors： Trapy G,Favre A,Esnault R

更新日期：1981-03-01 00:00:00

abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

journal_title：Plant molecular biology

authors： Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

更新日期：2008-04-01 00:00:00

abstract：:The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...

journal_title：Plant molecular biology

authors： Granger CL,Callos JD,Medford JI

更新日期：1996-05-01 00:00:00

abstract：:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

journal_title：Plant molecular biology

authors： Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

更新日期：2002-05-01 00:00:00

abstract：:The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...

journal_title：Plant molecular biology

authors： Fornalé S,Sonbol FM,Maes T,Capellades M,Puigdomènech P,Rigau J,Caparrós-Ruiz D

更新日期：2006-12-01 00:00:00

abstract：:Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...

journal_title：Plant molecular biology

authors： Takaiwa F,Yamanouchi U,Yoshihara T,Washida H,Tanabe F,Kato A,Yamada K

更新日期：1996-03-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...

journal_title：Plant molecular biology

authors： Breton C,Chaboud A,Matthys-Rochon E,Bates EE,Cock JM,Fromm H,Dumas C

更新日期：1995-01-01 00:00:00

abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

journal_title：Plant molecular biology

authors： Devitt ML,Maas KJ,Stafstrom JP

更新日期：1999-01-01 00:00:00

abstract：:The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...

journal_title：Plant molecular biology

authors： Niemeyer J,Ruhe J,Machens F,Stahl DJ,Hehl R

更新日期：2014-01-01 00:00:00

abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

journal_title：Plant molecular biology

authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

更新日期：2003-02-01 00:00:00

abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

journal_title：Plant molecular biology

authors： Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

更新日期：2014-12-01 00:00:00

abstract：:Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...

journal_title：Plant molecular biology

authors： Takaiwa F,Oono K,Wing D,Kato A

更新日期：1991-10-01 00:00:00

abstract：:We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...

journal_title：Plant molecular biology

authors： Jiménez S,Reighard GL,Bielenberg DG

更新日期：2010-05-01 00:00:00

abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

journal_title：Plant molecular biology

authors： Leone M,Zavallo D,Venturuzzi A,Asurmendi S

更新日期：2020-11-01 00:00:00

abstract：:We have sequenced and characterized the complete psbB gene cluster of Chlamydomonas reinhardtii chloroplast DNA. Although the petB and petD genes are located elsewhere, the sequential order of psbB, ORF31, psbN and psbH is identical to that of the psbB operon in higher plants. Also, intergenic non-coding regions are m...

journal_title：Plant molecular biology

authors： Johnson CH,Schmidt GW

更新日期：1993-07-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...

journal_title：Plant molecular biology

authors： Hong CE,Ha YI,Choi H,Moon JY,Lee J,Shin AY,Park CJ,Yoon GM,Kwon SY,Jo IH,Park JM

更新日期：2017-03-01 00:00:00

abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

journal_title：Plant molecular biology

authors： Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

更新日期：2016-01-01 00:00:00

abstract：KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...

journal_title：Plant molecular biology

authors： Yu SI,Kim H,Yun DJ,Suh MC,Lee BH

更新日期：2019-01-01 00:00:00

abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

journal_title：Plant molecular biology

authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

更新日期：1994-11-01 00:00:00

abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...

journal_title：Plant molecular biology

authors： Piechulla B,Merforth N,Rudolph B

更新日期：1998-11-01 00:00:00