Abstract:
:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in the absence of glucose. Six clones were characterized. One of them codes for AtGRH1, a new F-box protein that shows similarity to GRR1, a yeast protein involved in glucose repression. The ability of AtGRHI to activate glucose repression is dependent on the MIG1 repressor. Two-hybrid experiments revealed that AtGRH1 can interact with AtSKP1a and AtSKP1b, two recently identified SKP1 homologues in Arabidopsis. Other clones identified in the screen encode the transcription factor AtEBP, the 14-3-3 protein AtGF14 and two new proteins: AtMYR1 and AtPOZ1. None of these proteins turn on glucose repression. Instead, they illustrate various other ways by which foreign proteins can interfere with expression of a yeast gene. We conclude that our method worked as expected in at least one case, and that it could be applied to other signalling pathways that are conserved between yeast and higher eukaryotes.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne Hdoi
10.1023/a:1014440531842subject
Has Abstractpub_date
2002-05-01 00:00:00pages
69-79issue
1eissn
0167-4412issn
1573-5028journal_volume
49pub_type
杂志文章abstract::The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029654
更新日期:1984-07-01 00:00:00
abstract::Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499808317
更新日期:2000-06-01 00:00:00
abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00752901
更新日期:1986-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006058700720
更新日期:1998-06-01 00:00:00
abstract::The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019218
更新日期:1992-03-01 00:00:00
abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00040537
更新日期:1992-09-01 00:00:00
abstract::Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0173-1
更新日期:2014-05-01 00:00:00
abstract::TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1020719420867
更新日期:2003-01-01 00:00:00
abstract::Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006199003756
更新日期:1999-01-01 00:00:00
abstract::Electrophoretic analysis of translation products of polyadenylated RNA isolated from mid-maturation sorghum seed in the presence of [(35)S]met, [(3)H]leu, or [(3)H]val revealed two major proteins of kDa and 21 kDa. These products were not detected when [(3)H]lys was supplied as the radioactive substrate. Under similar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043202
更新日期:1989-03-01 00:00:00
abstract::Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expre...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0892-9
更新日期:2004-05-01 00:00:00
abstract:KEY MESSAGE:Moderate overexpression of CYP734A4 improves grain number per main panicle and seed setting rate. Brassinosteroid (BR) homeostasis and signaling are crucial for plant growth and development. CYP734A genes encode cytochrome P450 monooxygenases that control the level of bioactive BRs by degrading BRs. However...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0558-4
更新日期:2017-01-01 00:00:00
abstract::A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0023-x
更新日期:2006-06-01 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
abstract::Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9511-0
更新日期:2009-09-01 00:00:00
abstract::The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0122-4
更新日期:2014-01-01 00:00:00
abstract::Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026507517182
更新日期:2000-12-01 00:00:00
abstract::Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023427
更新日期:1991-04-01 00:00:00
abstract::Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0460-0
更新日期:2016-06-01 00:00:00
abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/B:PLAN.0000028797.63168.a7
更新日期:2004-01-01 00:00:00
abstract::The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0350-x
更新日期:2015-08-01 00:00:00
abstract:KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-00819-1
更新日期:2019-03-01 00:00:00
abstract::Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00039383
更新日期:1996-10-01 00:00:00
abstract::Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7533-1
更新日期:2005-01-01 00:00:00
abstract::An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040577
更新日期:1994-01-01 00:00:00
abstract::cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020607
更新日期:1996-04-01 00:00:00
abstract::As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0239-0
更新日期:2014-11-01 00:00:00
abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0324-z
更新日期:2015-06-01 00:00:00
abstract::Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006338401808
更新日期:1999-11-01 00:00:00
abstract::In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9619-2
更新日期:2010-05-01 00:00:00