Abstract：
:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a maize genome wide analysis of three Tourist-like MITE families, mPIF, and two previously uncharacterized families, ZmV1 and Zead8. We undertook a bioinformatic analysis of MITE insertion sites, developed methyl-sensitive transposon display (M-STD) assays to estimate the associated level of CpG methylation at MITE flanking regions, and conducted a population genetics approach to investigate MITE patterns of expansion. Our results reveal that the three MITE families insert into genomic regions that present specific molecular features: they are preferentially AT rich, present low level of cytosine methylation as compared to the LTR retrotransposon Grande, and target site duplications are flanked by large and conserved palindromic sequences. Moreover, the analysis of MITE distances from predicted genes shows that 73% of 263 copies are inserted at less than 5 kb from the nearest predicted gene, and copies from Zead8 family are significantly more abundant upstream of genes. By employing a population genetic approach we identified contrasting patterns of expansion among the three MITE families. All elements seem to have inserted roughly 1 million years ago but ZmV1 and Zead8 families present evidences for activity of several master copies within the last 0.4 Mya.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zerjal T,Joets J,Alix K,Grandbastien MA,Tenaillon MIdoi
10.1007/s11103-009-9511-0subject
Has Abstractpub_date
2009-09-01 00:00:00pages
99-114issue
1-2eissn
0167-4412issn
1573-5028journal_volume
71pub_type
杂志文章abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005736722794
更新日期：1997-04-01 00:00:00
abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023238
更新日期：1994-05-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1026597621646
更新日期：2000-11-01 00:00:00
abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014935
更新日期：1993-05-01 00:00:00
abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014544
更新日期：1993-02-01 00:00:00
abstract：:The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0317-y
更新日期：2015-06-01 00:00:00
abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021532
更新日期：1993-11-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037027
更新日期：1995-03-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0040-5
更新日期：2013-05-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0195-8
更新日期：2014-07-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9711-7
更新日期：2011-01-01 00:00:00
abstract：:A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9660-1
更新日期：2010-09-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037068
更新日期：1991-10-01 00:00:00
abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006148815106
更新日期：1999-02-01 00:00:00
abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014831302392
更新日期：2002-03-01 00:00:00
abstract：:The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00049336
更新日期：1996-02-01 00:00:00
abstract：:A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4208-5
更新日期：2006-02-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037051
更新日期：1991-10-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021430
更新日期：1993-10-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006035210889
更新日期：1998-07-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00036810
更新日期：1991-07-01 00:00:00
abstract：:The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension prot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014491
更新日期：1992-10-01 00:00:00
abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037557
更新日期：1985-11-01 00:00:00
abstract：:We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023248
更新日期：1994-05-01 00:00:00
abstract：:Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0445-z
更新日期：2016-05-01 00:00:00
abstract：:As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006081009173
更新日期：1998-11-01 00:00:00
abstract：KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00884-0
更新日期：2019-08-01 00:00:00
abstract：:Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9635-2
更新日期：2010-07-01 00:00:00
abstract：:RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0069-5
更新日期：2013-07-01 00:00:00
