Functional analysis of the grapevine paralogs of the SMG7 NMD factor using a heterolog VIGS-based gene depletion-complementation system.

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...

journal_title：Plant molecular biology

authors： Fung KL,Zhao KJ,He ZM,Chye ML

更新日期：2002-09-01 00:00:00

abstract：:The putative RNA helicase encoded by the Arabidopsis gene At1g32490 is a homolog of the yeast splicing RNA helicases Prp2 and Prp22. We isolated a temperature-sensitive allele (rsw12) of the gene in a screen for root radial swelling mutants. Plants containing this allele grown at the restrictive temperature showed wea...

journal_title：Plant molecular biology

authors： Howles PA,Gebbie LK,Collings DA,Varsani A,Broad RC,Ohms S,Birch RJ,Cork AH,Arioli T,Williamson RE

更新日期：2016-05-01 00:00:00

abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...

journal_title：Plant molecular biology

authors： Muñiz García MN,Muro MC,Mazzocchi LC,País SM,Stritzler M,Schlesinger M,Capiati DA

更新日期：2017-02-01 00:00:00

abstract：:Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...

journal_title：Plant molecular biology

authors： Jongsma M,Koornneef M,Zabel P,Hille J

更新日期：1987-09-01 00:00:00

abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...

journal_title：Plant molecular biology

authors： Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HE

更新日期：2014-07-01 00:00:00

abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

journal_title：Plant molecular biology

authors： Wei T,O'Connell MA

更新日期：1996-01-01 00:00:00

abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...

journal_title：Plant molecular biology

authors： Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler F

更新日期：1993-11-01 00:00:00

abstract：:Angiosperm genomes show tremendous variability in genome size and chromosome number. Nevertheless, comparative genetic mapping has revealed genome collinearity of closely related species. Sequence-based comparisons were used to assess the conservation of gene arrangements. Numerous small rearrangements, insertions/del...

journal_title：Plant molecular biology

authors： Schmidt R

更新日期：2002-01-01 00:00:00

abstract：:Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...

journal_title：Plant molecular biology

authors： Irish EE,McMurray D

更新日期：2006-03-01 00:00:00

abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

journal_title：Plant molecular biology

authors： Xue Y,Collin S,Davies DR,Thomas CM

更新日期：1994-04-01 00:00:00

abstract：:Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

journal_title：Plant molecular biology

authors： Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

更新日期：1986-07-01 00:00:00

abstract：:Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

journal_title：Plant molecular biology

authors： Luo L,Herrin DL

更新日期：2012-07-01 00:00:00

abstract：:Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

journal_title：Plant molecular biology

authors： Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

更新日期：2011-11-01 00:00:00

abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

journal_title：Plant molecular biology

authors： Xiang C,Miao ZH,Lam E

更新日期：1996-11-01 00:00:00

abstract：:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...

journal_title：Plant molecular biology

authors： O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JC

更新日期：1995-02-01 00:00:00

abstract：:Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...

journal_title：Plant molecular biology

authors： Yang P,Taoka K,Nakayama T,Iwabuchi M

更新日期：1995-04-01 00:00:00

abstract：:In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...

journal_title：Plant molecular biology

authors： Stenzel I,Hause B,Miersch O,Kurz T,Maucher H,Weichert H,Ziegler J,Feussner I,Wasternack C

更新日期：2003-04-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...

journal_title：Plant molecular biology

authors： Nessler CL,Burnett RJ

更新日期：1992-11-01 00:00:00

abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

journal_title：Plant molecular biology

authors： Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

更新日期：2002-02-01 00:00:00

abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...

journal_title：Plant molecular biology

authors： Sharma S,Holme IB,Dionisio G,Kodama M,Dzhanfezova T,Joernsgaard B,Brinch-Pedersen H

更新日期：2020-07-01 00:00:00

abstract：:The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...

journal_title：Plant molecular biology

authors： Spiker S,Everett KM

更新日期：1987-09-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...

journal_title：Plant molecular biology

authors： Chung KM,Sano H

更新日期：2007-12-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...

journal_title：Plant molecular biology

authors： Laxa M

更新日期：2017-01-01 00:00:00

abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...

journal_title：Plant molecular biology

authors： Luque I,Flores E,Herrero A

更新日期：1993-03-01 00:00:00

abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...

journal_title：Plant molecular biology

authors： Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L

更新日期：1991-09-01 00:00:00