Abstract:
:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining residues. All of the highly necrotic beta-elicitins have a hydrophilic residue (usually lysine) at position 13, whereas in the less necrotic alpha-elicitins this residue is replaced by a valine. Here, we report the synthesis and expression of a gene encoding a highly necrotic elicitin, beta-cryptogein, and we show that the substitution of Lys-13 of this recombinant protein by a valine leads to a drastic alteration to the necrotic activity of the recombinant protein.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JCdoi
10.1007/BF00019323subject
Has Abstractpub_date
1995-02-01 00:00:00pages
577-86issue
3eissn
0167-4412issn
1573-5028journal_volume
27pub_type
杂志文章abstract::In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014970
更新日期:1995-12-01 00:00:00
abstract::Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...
journal_title:Plant molecular biology
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doi:10.1007/s11103-010-9694-4
更新日期:2010-12-01 00:00:00
abstract::Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0082-8
更新日期:2013-10-01 00:00:00
abstract::Annexins belong to a multigene family of Ca(2+) dependent, phospholipid and cytoskeleton binding proteins. They have been shown to be upregulated under various stress conditions. We generated transgenic cotton plants expressing mustard annexin (AnnBj1), which showed enhanced tolerance towards different abiotic stress ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9615-6
更新日期:2010-06-01 00:00:00
abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0655-z
更新日期:2017-11-01 00:00:00
abstract::Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019506
更新日期:1994-12-01 00:00:00
abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-7307-4
更新日期:2005-07-01 00:00:00
abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014494
更新日期:1992-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017993
更新日期:1987-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020881
更新日期:1995-03-01 00:00:00
abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00914-x
更新日期:2019-11-01 00:00:00
abstract:KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01010-1
更新日期:2020-07-01 00:00:00
abstract::Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0173-1
更新日期:2014-05-01 00:00:00
abstract::Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020209
更新日期:1996-11-01 00:00:00
abstract::StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9462-5
更新日期:2009-05-01 00:00:00
abstract::Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023427
更新日期:1991-04-01 00:00:00
abstract::Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00015607
更新日期:1992-05-01 00:00:00
abstract::We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039423
更新日期:1990-12-01 00:00:00
abstract::During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...
journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
abstract::A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....
journal_title:Plant molecular biology
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doi:10.1007/s11103-006-9031-0
更新日期:2006-10-01 00:00:00
abstract::We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020506
更新日期:1989-02-01 00:00:00
abstract::Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014831302392
更新日期:2002-03-01 00:00:00
abstract::We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000023670.61059.1d
更新日期:2003-12-01 00:00:00
abstract::The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006369104530
更新日期:1999-10-01 00:00:00
abstract:KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0581-5
更新日期:2017-04-01 00:00:00
abstract::We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005916821224
更新日期:1998-03-01 00:00:00
abstract::The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006296422485
更新日期:1999-08-01 00:00:00
abstract::The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029879
更新日期:1988-07-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020244
更新日期:1995-05-01 00:00:00