Chemical synthesis, expression and mutagenesis of a gene encoding beta-cryptogein, an elicitin produced by Phytophthora cryptogea.

Abstract:

:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining residues. All of the highly necrotic beta-elicitins have a hydrophilic residue (usually lysine) at position 13, whereas in the less necrotic alpha-elicitins this residue is replaced by a valine. Here, we report the synthesis and expression of a gene encoding a highly necrotic elicitin, beta-cryptogein, and we show that the substitution of Lys-13 of this recombinant protein by a valine leads to a drastic alteration to the necrotic activity of the recombinant protein.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JC

doi

10.1007/BF00019323

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

577-86

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

27

pub_type

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