Abstract:
:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-expression of the CPN20 gene increases, but overexpression of the CPN20 gene reduces, ABA sensitivity in the major ABA responses including ABA-induced seed germination inhibition, postgermination growth arrest, promotion of stomatal closure and inhibition of stomatal opening. Genetic evidence supports that CPN20 functions downstream or at the same node of CHLH/ABAR, but upstream of the WRKY40 transcription factor. The other CPN20 interaction partners CPN10 and CPN60 are not involved in ABA signaling. Our findings show that CPN20 functions negatively in the ABAR-WRKY40 coupled ABA signaling independently of its co-chaperonin role, and provide a new insight into the role of co-chaperones in the regulation of plant responses to environmental cues.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zhang XF,Jiang T,Wu Z,Du SY,Yu YT,Jiang SC,Lu K,Feng XJ,Wang XF,Zhang DPdoi
10.1007/s11103-013-0082-8subject
Has Abstractpub_date
2013-10-01 00:00:00pages
205-18issue
3eissn
0167-4412issn
1573-5028journal_volume
83pub_type
杂志文章abstract::The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037145
更新日期:1991-11-01 00:00:00
abstract::Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041164
更新日期:1995-11-01 00:00:00
abstract::In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00033603
更新日期:1988-11-01 00:00:00
abstract::A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00034956
更新日期:1992-01-01 00:00:00
abstract::Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019312
更新日期:1995-02-01 00:00:00
abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9698-0
更新日期:2010-12-01 00:00:00
abstract::The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042359
更新日期:1993-12-01 00:00:00
abstract::Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042047
更新日期:1995-04-01 00:00:00
abstract::DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039524
更新日期:1994-10-01 00:00:00
abstract::Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9414-5
更新日期:2009-01-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01071-2
更新日期:2021-01-01 00:00:00
abstract::To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040693
更新日期:1994-11-01 00:00:00
abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020803
更新日期:1996-03-01 00:00:00
abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01114-8
更新日期:2021-01-22 00:00:00
abstract::In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1025070827979
更新日期:2003-07-01 00:00:00
abstract::A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0023-x
更新日期:2006-06-01 00:00:00
abstract::We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039532
更新日期:1994-10-01 00:00:00
abstract::The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0530-3
更新日期:2016-11-01 00:00:00
abstract:KEY MESSAGE:The induction of miR399 and miR398 and the inhibition of miR156, miR159, miR160, miR171, miR2111, and miR2643 were observed under Pi deficiency in alfalfa. The miRNA-mediated genes involved in basic metabolic process, root and shoot development, stress response and Pi uptake. Inorganic phosphate (Pi) defici...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0711-3
更新日期:2018-03-01 00:00:00
abstract::Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9297-5
更新日期:2008-04-01 00:00:00
abstract:KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00835-9
更新日期:2019-04-01 00:00:00
abstract::Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027384
更新日期:1988-05-01 00:00:00
abstract::Two S-adenosylmethionine synthetase (SAMS) cDNAs, PcSAMS1 and PcSAMS2, have been identified in Pinus contorta. We found that the two genes are differentially expressed during root development. Thus, PcSAMS1 is preferentially expressed in roots and exhibits a specific expression pattern in the meristem at the onset of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010637012528
更新日期:2001-06-01 00:00:00
abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037027
更新日期:1995-03-01 00:00:00
abstract::The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023557
更新日期:1994-02-01 00:00:00
abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0799-5
更新日期:2019-01-01 00:00:00
abstract::Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9585-8
更新日期:2010-03-01 00:00:00
abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015971
更新日期:1993-06-01 00:00:00