Abstract：
:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish positive interactions with Rhizobium and fungi (NOD(-), MYC(-)); in particular, it does not recognize signal molecules released by symbiotic micro-organisms so that eventually, epidermal cells undergo PCD at the contact area. We performed a detailed characterization of wild-type and Ljsym4-2 cultured cells by taking into account several parameters characterizing cell responses to SA, a molecule strongly involved in defense signaling pathways. In the presence of 0.5 mM SA, Ljsym4-2 suspension-cultured cells reduce their growth and eventually die, whereas in order to induce the same effects in wt suspension cells, SA concentration must be raised to 1.5 mM. An early and short production of nitric oxide (NO) and reactive oxygen species (ROS) was detected in wt-treated cells. In contrast, a continuous production of NO and a double-peak ROS response, similar to that reported after a pathogenic attack, was observed in the mutant Ljsym4-2 cells. At the molecular level, a constitutive higher level of a SA-inducible pathogenesis related gene was observed. The analysis in planta revealed a strong induction of the LjPR1 gene in the Ljsym4-2 mutant inoculated with Mesorhizobium loti.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo Fdoi
10.1007/s11103-009-9585-8subject
Has Abstractpub_date
2010-03-01 00:00:00pages
469-83issue
4-5eissn
0167-4412issn
1573-5028journal_volume
72pub_type
杂志文章abstract：:Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...
journal_title：Plant molecular biology
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更新日期：1997-11-01 00:00:00
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journal_title：Plant molecular biology
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更新日期：1998-10-01 00:00:00
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