Abstract:
:We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 residues with a calculated molecular mass of 11 kDa and a typical N-terminal signal sequence. The protein has about 30% identity with various Bowman-Birk type proteinase inhibitors. Most interestingly, it is novel in that it is double-headed with exclusive specificity for chymotrypsin. WIP1 is strongly wound-induced in contrast to other members of the Bowman-Birk proteinase inhibitor family, which occur in seeds and are regulated during development. The response is fast, similar to defence-induced genes, and measurable as early as 30 min after wounding. Induction can also be evoked in the intact coleoptiles and the signal is systematically transmitted in the coleoptile to adjacent regions of the wounded area. Isolation and analysis of the corresponding genomic clone reveals that WIP1 contains an intron of 90 nucleotides.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Rohrmeier T,Lehle Ldoi
10.1007/BF00027365subject
Has Abstractpub_date
1993-08-01 00:00:00pages
783-92issue
5eissn
0167-4412issn
1573-5028journal_volume
22pub_type
杂志文章abstract::Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00015607
更新日期:1992-05-01 00:00:00
abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006099512706
更新日期:1998-06-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract::Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028803
更新日期:1993-02-01 00:00:00
abstract::Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7533-1
更新日期:2005-01-01 00:00:00
abstract::In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037027
更新日期:1995-03-01 00:00:00
abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5620-6
更新日期:2006-03-01 00:00:00
abstract::A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005897921567
更新日期:1997-12-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020626
更新日期:1985-09-01 00:00:00
abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0468-5
更新日期:2016-06-01 00:00:00
abstract::The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042040
更新日期:1995-04-01 00:00:00
abstract::The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1026540524990
更新日期:2000-10-01 00:00:00
abstract::RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005818631401
更新日期:1997-06-01 00:00:00
abstract::TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024193
更新日期:1994-04-01 00:00:00
abstract::The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049336
更新日期:1996-02-01 00:00:00
abstract::Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01578377
更新日期:1983-07-01 00:00:00
abstract::The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-6235-z
更新日期:2004-12-01 00:00:00
abstract::Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027384
更新日期:1988-05-01 00:00:00
abstract::SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005875300606
更新日期:1997-07-01 00:00:00
abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0156-7
更新日期:2014-03-01 00:00:00
abstract::14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021261614491
更新日期:2002-12-01 00:00:00
abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00166458
更新日期:1987-05-01 00:00:00
abstract::The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016430
更新日期:1987-01-01 00:00:00
abstract::The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023991
更新日期:1991-03-01 00:00:00
abstract::Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006178710443
更新日期:1999-01-01 00:00:00
abstract::Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0546-8
更新日期:2017-01-01 00:00:00
abstract::Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014948
更新日期:1988-09-01 00:00:00
abstract::Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005968901298
更新日期:1998-05-01 00:00:00