Abstract：
:RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cytoplasm maize mitochondria. A soluble complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in mitochondrial lysates, was identified. The complex had a buoyant density of 1.49 g/cm3, indicating a substantial non-nucleic acids content. The sedimentation coefficient of the complex, however, was only slightly larger than that of deproteinized RNA b. Synthesis of RNA b as well as the larger RNA plasmid, RNA a, was resistant to heparin, suggesting that, for both RNAs, preformed complexes between an RNA template and an RNA-dependent RNA polymerase capable of elongating in vivo preinitiated RNA plasmid strands, were present in the lysate. Only a small fraction of RNA b molecules were bound in the complex; the bulk of RNA b sedimented at the same rate as the deproteinized RNA. Thus, after replication, maize mitochondrial plasmids are not associated with nucleoprotein capsids although their synthesis takes place through ribonucleoprotein replication complexes.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Formanová N,Brown GGdoi
10.1023/a:1005818631401subject
Has Abstractpub_date
1997-06-01 00:00:00pages
383-92issue
3eissn
0167-4412issn
1573-5028journal_volume
34pub_type
杂志文章abstract：:A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020464
更新日期：1995-12-01 00:00:00
abstract：:A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023388
更新日期：1992-06-01 00:00:00
abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039430
更新日期：1990-12-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019323
更新日期：1995-02-01 00:00:00
abstract：:Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-008-9374-9
更新日期：2009-03-01 00:00:00
abstract：:Lysine residue 9 of histone H3 can either be acetylated or mono-, di-, or tri-methylated. These epigenetic states have a diverse impact on regulating gene transcriptional activity and chromatin organization. H3K9ac is invariably correlated with transcriptional activation, whereas H3K9me2 has been reported to be mainly...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9594-7
更新日期：2010-04-01 00:00:00
abstract：:Angiosperm genomes show tremendous variability in genome size and chromosome number. Nevertheless, comparative genetic mapping has revealed genome collinearity of closely related species. Sequence-based comparisons were used to assess the conservation of gene arrangements. Numerous small rearrangements, insertions/del...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:Annexins belong to a multigene family of Ca(2+) dependent, phospholipid and cytoskeleton binding proteins. They have been shown to be upregulated under various stress conditions. We generated transgenic cotton plants expressing mustard annexin (AnnBj1), which showed enhanced tolerance towards different abiotic stress ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9615-6
更新日期：2010-06-01 00:00:00
abstract：:A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005830313272
更新日期：1997-10-01 00:00:00
abstract：KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00835-9
更新日期：2019-04-01 00:00:00
abstract：:A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01595169
更新日期：1983-03-01 00:00:00
abstract：:Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019389
更新日期：1990-06-01 00:00:00
abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00187572
更新日期：1983-01-01 00:00:00
abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040693
更新日期：1994-11-01 00:00:00
abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-7533-1
更新日期：2005-01-01 00:00:00
abstract：:The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9190-7
更新日期：2007-09-01 00:00:00
abstract：:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00032673
更新日期：1995-09-01 00:00:00
abstract：:StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9462-5
更新日期：2009-05-01 00:00:00
abstract：:Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interact...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9639-y
更新日期：2010-07-01 00:00:00
abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578377
更新日期：1983-07-01 00:00:00
abstract：:We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000023670.61059.1d
更新日期：2003-12-01 00:00:00
abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006040306581
更新日期：1998-10-01 00:00:00
abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015670
更新日期：1988-03-01 00:00:00
abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005784115363
更新日期：1997-01-01 00:00:00
abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006031822141
更新日期：1998-07-01 00:00:00
abstract：:The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014183
更新日期：1987-01-01 00:00:00
abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418760
更新日期：1985-03-01 00:00:00
abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00034956
更新日期：1992-01-01 00:00:00
