Intron-mediated enhancement of heterologous gene expression in maize.

Abstract:

:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the inclusion of maize alcohol dehydrogenase-1 introns 2 and 6 in the chimeric construct. This enhancement was seen when the intron was placed within the 5' translated region but not when it was located upstream of the promoter or within the 3' untranslated region. Deletion of exon sequences adjacent to intron 2 abolished its ability to mediate enhancement of CAT gene expression. Northern analysis of protoplasts electroporated with intron constructs revealed elevated levels of CAT mRNA. However, this elevation was insufficient to account for the increased enzyme activity. One explanation of these results is that splicing affects both the quantity and quality of mRNA.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

doi

10.1007/BF00039430

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

913-20

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

15

pub_type

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