Gene expression during anthesis and senescence in Iris flowers.

Abstract:

:We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and DNA coiling, and with morphological data. Plasmodesmata of mesophyll cells closed about two days before flower opening, while in the epidermis they closed concomitant with opening. Similarly, the onset of visible senescence in the epidermis cells occurred about two days later than in the mesophyll. About 1400 PCR-amplified clones, derived from a subtractive cDNA library enriched for tepal-specific genes, were spotted and about 240 clones, including 200 that were expressed most differentially, were sequenced. The expression patterns showed three main clusters. One exhibited high expression during tepal growth (cluster A). These genes were putatively associated with pigmentation, cell wall synthesis and metabolism of lipids and proteins. The second cluster (B) was highly expressed during flower opening. The third cluster (C) related to the final stages of senescence, with genes putatively involved in signal transduction, and the remobilization of phospholipids, proteins, and cell wall compounds. Throughout the sampling period, numerous plant defence genes were highly expressed. We identified an ion channel protein putatively involved in senescence, and some putative regulators of transcription and translation, including a MADS-domain factor.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

van Doorn WG,Balk PA,van Houwelingen AM,Hoeberichts FA,Hall RD,Vorst O,van der Schoot C,van Wordragen MF

doi

10.1023/B:PLAN.0000023670.61059.1d

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

845-63

issue

6

eissn

0167-4412

issn

1573-5028

pii

5265268

journal_volume

53

pub_type

杂志文章
  • Five novel transcription factors as potential regulators of OsNHX1 gene expression in a salt tolerant rice genotype.

    abstract:KEY MESSAGE:This manuscript reports the identification and characterization of five transcription factors binding to the promoter of OsNHX1 in a salt stress tolerant rice genotype (Hasawi). Although NHX1 encoding genes are known to be highly regulated at the transcription level by different abiotic stresses, namely sal...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0547-7

    authors: Almeida DM,Gregorio GB,Oliveira MM,Saibo NJ

    更新日期:2017-01-01 00:00:00

  • Molecular cloning, structure and expression of an elicitor-inducible chitinase gene from pine trees.

    abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005708611020

    authors: Wu H,Echt CS,Popp MP,Davis JM

    更新日期:1997-04-01 00:00:00

  • Isolation, characterization and expression of the maize Cat2 catalase gene.

    abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020803

    authors: Guan L,Polidoros AN,Scandalios JG

    更新日期:1996-03-01 00:00:00

  • In silico analysis of the sequence features responsible for alternatively spliced introns in the model green alga Chlamydomonas reinhardtii.

    abstract::Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0605-9

    authors: Raj-Kumar PK,Vallon O,Liang C

    更新日期:2017-06-01 00:00:00

  • The soybean ENOD40(2) promoter is active in Arabidopsis thaliana and is temporally and spatially regulated.

    abstract::The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006146627301

    authors: Mirabella R,Martirani L,Lamberti A,Iaccarino M,Chiurazzi M

    更新日期:1999-01-01 00:00:00

  • Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.

    abstract::A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041395

    authors: Kim J,Leustek T

    更新日期:1996-12-01 00:00:00

  • Expression analysis of a sucrose carrier in the germinating seedling of Ricinus communis.

    abstract::This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006040306581

    authors: Bick JA,Neelam A,Smith E,Nelson SJ,Hall JL,Williams LE

    更新日期:1998-10-01 00:00:00

  • Extensive alterations in DNA methylation and transcription in rice caused by introgression from Zizania latifolia.

    abstract::It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000038270.48326.7a

    authors: Liu Z,Wang Y,Shen Y,Guo W,Hao S,Liu B

    更新日期:2004-03-01 00:00:00

  • Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

    abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020464

    authors: Espartero J,Sánchez-Aguayo I,Pardo JM

    更新日期:1995-12-01 00:00:00

  • Polymorphism of mitochondrial DNA 'S' regions among normal cytoplasms of maize.

    abstract::Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF01578377

    authors: McNay JW,Pring DR,Lonsdale DM

    更新日期:1983-07-01 00:00:00

  • Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis.

    abstract::APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9711-7

    authors: Krishnaswamy S,Verma S,Rahman MH,Kav NN

    更新日期:2011-01-01 00:00:00

  • Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana.

    abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1016047231597

    authors: Osakabe K,Yoshioka T,Ichikawa H,Toki S

    更新日期:2002-09-01 00:00:00

  • Organization and transcription of the class I phycoerythrin genes of the marine cyanobacterium Synechococcus sp. WH7803.

    abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023564

    authors: Newman J,Mann NH,Carr NG

    更新日期:1994-02-01 00:00:00

  • The elongation defective1 mutant of Arabidopsis is impaired in the gene encoding a serine-rich secreted protein.

    abstract::Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000019067.05185.d6

    authors: Lertpiriyapong K,Sung ZR

    更新日期:2003-11-01 00:00:00

  • Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos.

    abstract::The complete nucleotide sequence of the Chlamydomonas eugametos (Chlamydomonadales, Chlorophyceae, sensu Mattox and Stewart) mitochondrial genome has been determined (22,897 bp, 34.6% G + C). The genes identified in this circular-mapping genome include those for apocytochrome b, subunit 1 of the cytochrome oxidase com...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005995718091

    authors: Denovan-Wright EM,Nedelcu AM,Lee RW

    更新日期:1998-01-01 00:00:00

  • Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance.

    abstract::Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000028775.58537.fe

    authors: Gachon C,Baltz R,Saindrenan P

    更新日期:2004-01-01 00:00:00

  • Genomic organization and evolution of the soybean SB92 satellite sequence.

    abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041174

    authors: Vahedian M,Shi L,Zhu T,Okimoto R,Danna K,Keim P

    更新日期:1995-11-01 00:00:00

  • The plant transcription factor TGA1 stimulates expression of the CaMV 35S promoter in Saccharomyces cerevisiae.

    abstract::We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023248

    authors: Rüth J,Schweyen RJ,Hirt H

    更新日期:1994-05-01 00:00:00

  • Berberine enhances defects in the establishment of leaf polarity in asymmetric leaves1 and asymmetric leaves2 of Arabidopsis thaliana.

    abstract::Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9929-7

    authors: Nakagawa A,Takahashi H,Kojima S,Sato N,Ohga K,Cha BY,Woo JT,Nagai K,Horiguchi G,Tsukaya H,Machida Y,Machida C

    更新日期:2012-08-01 00:00:00

  • Phenotypic and gene expression analyses of a ploidy series of maize inbred Oh43.

    abstract::Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9722-4

    authors: Yao H,Kato A,Mooney B,Birchler JA

    更新日期:2011-02-01 00:00:00

  • Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis.

    abstract::The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0459-6

    authors: Yamaguchi T,Noge K,Asano Y

    更新日期:2016-06-01 00:00:00

  • Cellular double-stranded RNA in Phaseolus vulgaris.

    abstract::High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017873

    authors: Wakarchuk DA,Hamilton RI

    更新日期:1985-01-01 00:00:00

  • Caught in action: fine-scale plastome evolution in the parasitic plants of Cuscuta section Ceratophorae (Convolvulaceae).

    abstract:KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00884-0

    authors: Banerjee A,Stefanović S

    更新日期:2019-08-01 00:00:00

  • Over-expressing a yeast ornithine decarboxylase gene in transgenic roots of Nicotiana rustica can lead to enhanced nicotine accumulation.

    abstract::Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017721

    authors: Hamill JD,Robins RJ,Parr AJ,Evans DM,Furze JM,Rhodes MJ

    更新日期:1990-07-01 00:00:00

  • Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents.

    abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027351

    authors: Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

    更新日期:1992-05-01 00:00:00

  • Comparative analysis of the nucleosomal structure of rye, wheat and their relatives.

    abstract::Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005912822671

    authors: Vershinin AV,Heslop-Harrison JS

    更新日期:1998-01-01 00:00:00

  • RdDM pathway components differentially modulate Tobamovirus symptom development.

    abstract:KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01051-6

    authors: Leone M,Zavallo D,Venturuzzi A,Asurmendi S

    更新日期:2020-11-01 00:00:00

  • A cDNA clone encoding Brassica calmodulin.

    abstract::A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020195

    authors: Chye ML,Liu CM,Tan CT

    更新日期:1995-01-01 00:00:00

  • Enhancing disease resistance in poplar through modification of its natural defense pathway.

    abstract:KEY MESSAGE:Modification of the poplar defense pathway through pathogen-induced expression of an amphibian host defense peptide modulates plant innate immunity and confers robust and reliable resistance against a major poplar pathogen, Septoria musiva. Host defense peptides (HDPs), also known as cationic antimicrobial ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00874-2

    authors: Yevtushenko DP,Misra S

    更新日期:2019-07-01 00:00:00

  • Identification of genes preferentially expressed during wood formation in Eucalyptus.

    abstract::Wood is the most abundant biological resource on earth and it is also an important raw material for a major global industry with rapidly increasing demand. The genus Eucalyptus includes the most widely used tree species for industrial plantation, mainly for making pulp and paper. With the aim of identifying major gene...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-0621-4

    authors: Paux E,Tamasloukht M,Ladouce N,Sivadon P,Grima-Pettenati J

    更新日期:2004-05-01 00:00:00