Abstract:
:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equivalents. The library is organised as an ordered array in duplicate microtitre plates. Forty-one pools of DNA from 1920 individual clones have been prepared for rapid screening of the library by the polymerase chain reaction (PCR). Using this approach, together with conventional colony hybridisation, we have been able to identify between one and eight positive clones for every probe used.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch Wdoi
10.1007/BF00027351subject
Has Abstractpub_date
1992-05-01 00:00:00pages
299-308issue
2eissn
0167-4412issn
1573-5028journal_volume
19pub_type
杂志文章abstract::Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9984-0
更新日期:2013-01-01 00:00:00
abstract:KEY MESSAGE:Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls. Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0702-4
更新日期:2018-04-01 00:00:00
abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023564
更新日期:1994-02-01 00:00:00
abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000009269.97773.70
更新日期:2003-09-01 00:00:00
abstract::In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9785-x
更新日期:2011-08-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022324920316
更新日期:2003-03-01 00:00:00
abstract::Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0054-3
更新日期:2006-08-01 00:00:00
abstract::TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024193
更新日期:1994-04-01 00:00:00
abstract:KEY MESSAGE:AFP improved cryopreservation efficiency of potato (Solanum tuberosum cv. Superior) by regulating transcript levels of CBF1 and DHN1. However, the optimal AFP concentration required for strong induction of the genes was dependent on the type of vitrification solution to which the AFP was added: This finding...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0743-8
更新日期:2018-07-01 00:00:00
abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00166458
更新日期:1987-05-01 00:00:00
abstract::Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9380-y
更新日期:2009-03-01 00:00:00
abstract::Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9943-9
更新日期:2012-09-01 00:00:00
abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040716
更新日期:1996-08-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043874
更新日期:1994-06-01 00:00:00
abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016019
更新日期:1989-12-01 00:00:00
abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0320-3
更新日期:2015-06-01 00:00:00
abstract::A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these tra...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040703
更新日期:1994-11-01 00:00:00
abstract:KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01010-1
更新日期:2020-07-01 00:00:00
abstract:KEY MESSAGE:We identified three dormant stages of Polygonatum kingianum and changes that occurred during dormancy transition in the following aspects including cell wall and hormones, as well as interaction among them. Polygonatum kingianum Coll.et Hemsl (P. kingianum) is an important traditional Chinese medicine, but ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0812-z
更新日期:2019-02-01 00:00:00
abstract::The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9239-7
更新日期:2007-12-01 00:00:00
abstract::Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020236
更新日期:1995-02-01 00:00:00
abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021807
更新日期:1996-05-01 00:00:00
abstract::Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0181-1
更新日期:2014-06-01 00:00:00
abstract::Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0106-4
更新日期:2013-12-01 00:00:00
abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0655-z
更新日期:2017-11-01 00:00:00
abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
更新日期:1993-09-01 00:00:00
abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005909703600
更新日期:1998-05-01 00:00:00