Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents.

Abstract:

:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equivalents. The library is organised as an ordered array in duplicate microtitre plates. Forty-one pools of DNA from 1920 individual clones have been prepared for rapid screening of the library by the polymerase chain reaction (PCR). Using this approach, together with conventional colony hybridisation, we have been able to identify between one and eight positive clones for every probe used.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

doi

10.1007/BF00027351

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

299-308

issue

2

eissn

0167-4412

issn

1573-5028

journal_volume

19

pub_type

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