Abstract:
:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization data with S1 and S2 DNAs and with two cosmids spanning these regions were consistent with the concept that S1 and S2 sequences are found in each normal cytoplasm. Three variations of the S1 region were established; the Wf9 structure, a second group consisting of F6, A188, and W182BN, and a third, Black Mexican. Genome structure was conserved through the S2 region in all lines examined. None of the cytoplasms included complete copies of S1; the 1400 bp repeat characteristic of S1 and S2 was absent in the S1 region of all lines. A 2.1 kb linear DNA was observed instead of a 2.3 kb DNA in F6, A188, and W182BN. Integrated copies of S1 and S2 sequences may be a constituitive characteristic of normal, male-fertile maize cytoplasms.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
McNay JW,Pring DR,Lonsdale DMdoi
10.1007/BF01578377subject
Has Abstractpub_date
1983-07-01 00:00:00pages
177-87issue
4eissn
0167-4412issn
1573-5028journal_volume
2pub_type
杂志文章abstract::Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1998-09-01 00:00:00
abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000009269.97773.70
更新日期:2003-09-01 00:00:00
abstract::Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0187-8
更新日期:2014-06-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00044158
更新日期:1989-06-01 00:00:00
abstract::The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005784115363
更新日期:1997-01-01 00:00:00
abstract::In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4922-z
更新日期:2006-03-01 00:00:00
abstract::Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020176
更新日期:1994-01-01 00:00:00
abstract::The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006058700720
更新日期:1998-06-01 00:00:00
abstract::The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006355510883
更新日期:2000-03-01 00:00:00
abstract::The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021797
更新日期:1996-05-01 00:00:00
abstract::Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0140-2
更新日期:2014-03-01 00:00:00
abstract::Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023427
更新日期:1991-04-01 00:00:00
abstract:KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0767-0
更新日期:2018-09-01 00:00:00
abstract::Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006138221874
更新日期:1999-03-01 00:00:00
abstract::Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9071-5
更新日期:2007-01-01 00:00:00
abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0799-5
更新日期:2019-01-01 00:00:00
abstract::Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9441-2
更新日期:2009-03-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016064
更新日期:1984-05-01 00:00:00
abstract::Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029145
更新日期:1992-10-01 00:00:00
abstract:KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00835-9
更新日期:2019-04-01 00:00:00
abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9374-9
更新日期:2009-03-01 00:00:00
abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028864
更新日期:1994-11-01 00:00:00
abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016019
更新日期:1989-12-01 00:00:00
abstract::Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043653
更新日期:1995-10-01 00:00:00
abstract::A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020226
更新日期:1995-02-01 00:00:00
abstract::The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-4679-9
更新日期:2004-11-01 00:00:00
abstract::The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9190-7
更新日期:2007-09-01 00:00:00
abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5101-y
更新日期:2005-06-01 00:00:00