Abstract：
:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tissues analysed, higher levels of expression were found in leaves and stem than in roots and tubers. For both genes, no significant differences in level of expression could be detected under a variety of conditions such as cold treatment, anaerobiosis, sucrose induction or treatment with a synthetic cytokinin. Only 2,4-D and prolonged periods of darkness lead to a slight reduction in mRNA levels. The reduction in darkness was compensated after transfer of the plants back into the light. Expression of the ATPase genes remained constant in transgenic plants which are inhibited in phloem loading due to antisense inhibition of the sucrose transporter. On the other hand, expression of the sucrose transporter is inducible by auxin and cytokinin but not by sucrose. Taken together, these data suggest that at least the two plasma membrane H(+)-ATPase genes analysed are rather constant in their expression and that either other genes respond to external stimuli or that most of the regulation occurs at the posttranscriptional level.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Harms K,Wöhner RV,Schulz B,Frommer WBdoi
10.1007/BF00028864subject
Has Abstractpub_date
1994-11-01 00:00:00pages
979-88issue
3eissn
0167-4412issn
1573-5028journal_volume
26pub_type
杂志文章abstract：:A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9445-y
更新日期：2009-04-01 00:00:00
abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9204-5
更新日期：2007-09-01 00:00:00
abstract：:Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9621-8
更新日期：2010-05-01 00:00:00
abstract：:To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005839832096
更新日期：1997-05-01 00:00:00
abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040137
更新日期：1986-03-01 00:00:00
abstract：:The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00043874
更新日期：1994-06-01 00:00:00
abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0233-6
更新日期：2014-11-01 00:00:00
abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042240
更新日期：1996-06-01 00:00:00
abstract：:We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/b:plan.0000006936.50532.3a
更新日期：2003-10-01 00:00:00
abstract：KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0728-7
更新日期：2018-05-01 00:00:00
abstract：:The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010665831955
更新日期：2001-05-01 00:00:00
abstract：:We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019293
更新日期：1993-11-01 00:00:00
abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1021162321527
更新日期：2003-01-01 00:00:00
abstract：:Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-008-9374-9
更新日期：2009-03-01 00:00:00
abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0240-7
更新日期：2014-11-01 00:00:00
abstract：:Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/b:plan.0000009693.26740.c3
更新日期：1997-01-01 00:00:00
abstract：:Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9943-9
更新日期：2012-09-01 00:00:00
abstract：:The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023992
更新日期：1991-03-01 00:00:00
abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01037-4
更新日期：2020-09-01 00:00:00
abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015678
更新日期：1985-05-01 00:00:00
abstract：:Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005857717196
更新日期：1997-12-01 00:00:00
abstract：:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0459-6
更新日期：2016-06-01 00:00:00
abstract：:A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00043873
更新日期：1994-06-01 00:00:00
abstract：:Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9041-y
更新日期：2006-11-01 00:00:00
abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039617
更新日期：1993-01-01 00:00:00
abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00047719
更新日期：1992-04-01 00:00:00
abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040716
更新日期：1996-08-01 00:00:00
abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2525-8
更新日期：2004-09-01 00:00:00
abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1026540524990
更新日期：2000-10-01 00:00:00
abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0040-5
更新日期：2013-05-01 00:00:00
