Isolation and characterization of P-type H(+)-ATPase genes from potato.


:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tissues analysed, higher levels of expression were found in leaves and stem than in roots and tubers. For both genes, no significant differences in level of expression could be detected under a variety of conditions such as cold treatment, anaerobiosis, sucrose induction or treatment with a synthetic cytokinin. Only 2,4-D and prolonged periods of darkness lead to a slight reduction in mRNA levels. The reduction in darkness was compensated after transfer of the plants back into the light. Expression of the ATPase genes remained constant in transgenic plants which are inhibited in phloem loading due to antisense inhibition of the sucrose transporter. On the other hand, expression of the sucrose transporter is inducible by auxin and cytokinin but not by sucrose. Taken together, these data suggest that at least the two plasma membrane H(+)-ATPase genes analysed are rather constant in their expression and that either other genes respond to external stimuli or that most of the regulation occurs at the posttranscriptional level.


Plant Mol Biol


Plant molecular biology


Harms K,Wöhner RV,Schulz B,Frommer WB




Has Abstract


1994-11-01 00:00:00












  • CR1 - a dispersed repeated element associated with the Cab-1 locus in tomato.

    abstract::Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bernatzky R,Pichersky E,Malik VS,Tanksley SD

    更新日期:1988-09-01 00:00:00

  • Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

    abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Packer JC,André D,Howe CJ

    更新日期:1995-01-01 00:00:00

  • The homeodomain-leucine zipper (HD-Zip) class I transcription factors ATHB7 and ATHB12 modulate abscisic acid signalling by regulating protein phosphatase 2C and abscisic acid receptor gene activities.

    abstract::Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Valdés AE,Overnäs E,Johansson H,Rada-Iglesias A,Engström P

    更新日期:2012-11-01 00:00:00

  • Early genomic responses to salicylic acid in Arabidopsis.

    abstract::Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

    更新日期:2009-05-01 00:00:00

  • Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.

    abstract::The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bischoff F,Vahlkamp L,Molendijk A,Palme K

    更新日期:2000-02-01 00:00:00

  • Profiling methyl jasmonate-responsive transcriptome for understanding induced systemic resistance in whitebark pine (Pinus albicaulis).

    abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Liu JJ,Williams H,Li XR,Schoettle AW,Sniezko RA,Murray M,Zamany A,Roke G,Chen H

    更新日期:2017-11-01 00:00:00

  • ZmGOLS2, a target of transcription factor ZmDREB2A, offers similar protection against abiotic stress as ZmDREB2A.

    abstract::GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

    更新日期:2016-01-01 00:00:00

  • Light induces accumulation of isocitrate lyase mRNA in a carotenoid-deficient mutant of Chlamydomonas reinhardtii.

    abstract::A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Petridou S,Foster K,Kindle K

    更新日期:1997-02-01 00:00:00

  • Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea.

    abstract::In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Gaude T,Rougier M,Heizmann P,Ockendon DJ,Dumas C

    更新日期:1995-03-01 00:00:00

  • Identification and characterisation of the alpha and beta subunits of succinyl CoA ligase of tomato.

    abstract::Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Studart-Guimarães C,Gibon Y,Frankel N,Wood CC,Zanor MI,Fernie AR,Carrari F

    更新日期:2005-11-01 00:00:00

  • The β-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the α-subunit genes.

    abstract::β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

    更新日期:2012-02-01 00:00:00

  • GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress.

    abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ni Z,Hu Z,Jiang Q,Zhang H

    更新日期:2013-05-01 00:00:00

  • Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.

    abstract::The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Xiang C,Miao ZH,Lam E

    更新日期:1996-11-01 00:00:00

  • A temperature-sensitive allele of a putative mRNA splicing helicase down-regulates many cell wall genes and causes radial swelling in Arabidopsis thaliana.

    abstract::The putative RNA helicase encoded by the Arabidopsis gene At1g32490 is a homolog of the yeast splicing RNA helicases Prp2 and Prp22. We isolated a temperature-sensitive allele (rsw12) of the gene in a screen for root radial swelling mutants. Plants containing this allele grown at the restrictive temperature showed wea...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Howles PA,Gebbie LK,Collings DA,Varsani A,Broad RC,Ohms S,Birch RJ,Cork AH,Arioli T,Williamson RE

    更新日期:2016-05-01 00:00:00

  • Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements.

    abstract::Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

    更新日期:1995-09-01 00:00:00

  • A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.

    abstract::Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Luo L,Herrin DL

    更新日期:2012-07-01 00:00:00

  • Zrp2: a novel maize gene whose mRNA accumulates in the root cortex and mature stems.

    abstract::A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

    更新日期:1997-10-01 00:00:00

  • Hormone interactions during vascular development.

    abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Dettmer J,Elo A,Helariutta Y

    更新日期:2009-03-01 00:00:00

  • Characterisation of a wound-induced transcript from the monocot asparagus that shares similarity with a class of intracellular pathogenesis-related (PR) proteins.

    abstract::We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Warner SA,Scott R,Draper J

    更新日期:1992-07-01 00:00:00

  • Phytochrome regulation of mRNA levels of ribulose-1,5-bisphosphate carboxylase in etiolated rye seedlings (Secale cereale).

    abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

    更新日期:1987-01-01 00:00:00

  • Context sequences of translation initiation codon in plants.

    abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Joshi CP,Zhou H,Huang X,Chiang VL

    更新日期:1997-12-01 00:00:00

  • Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein.

    abstract::Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kuroda I,Inagaki J,Yamamoto Y

    更新日期:1993-01-01 00:00:00

  • A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum.

    abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

    更新日期:1993-03-01 00:00:00

  • Flavonoid profiling among wild type and related GM wheat varieties.

    abstract::Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ioset JR,Urbaniak B,Ndjoko-Ioset K,Wirth J,Martin F,Gruissem W,Hostettmann K,Sautter C

    更新日期:2007-11-01 00:00:00

  • Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

    abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Atanassova R,Flénet M,Gigot C,Chaubet N

    更新日期:1998-05-01 00:00:00

  • Structure and expression of the barley lipid transfer protein gene Ltp1.

    abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

    更新日期:1992-02-01 00:00:00

  • Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz 'KU50' achieves enzyme-active heteromeric complex formation.

    abstract:KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC., a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Panpetch P,Field RA,Limpaseni T

    更新日期:2018-03-01 00:00:00

  • SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis.

    abstract::Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zhang Y,Schwarz S,Saedler H,Huijser P

    更新日期:2007-02-01 00:00:00

  • Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants.

    abstract::Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

    更新日期:1987-11-01 00:00:00

  • A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and physiological responses.

    abstract::A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Deng X,Phillips J,Bräutigam A,Engström P,Johannesson H,Ouwerkerk PB,Ruberti I,Salinas J,Vera P,Iannacone R,Meijer AH,Bartels D

    更新日期:2006-06-01 00:00:00