Organization, not duplication, triggers silencing in a complex transgene locus in rice.

abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...

journal_title：Plant molecular biology

authors： Saint-Guily A,Schantz ML,Schantz R

更新日期：1994-03-01 00:00:00

abstract：:The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...

journal_title：Plant molecular biology

authors： Fornalé S,Sonbol FM,Maes T,Capellades M,Puigdomènech P,Rigau J,Caparrós-Ruiz D

更新日期：2006-12-01 00:00:00

abstract：:Due to an unfortunate turn of events, the funding note for Open Access publication was not properly provided in the original publication. Hence, the original article has been corrected. The opening line of the Acknowledgement section should read. ...

journal_title：Plant molecular biology

authors： Luck K,Jia Q,Huber M,Handrick V,Wong GK,Nelson DR,Chen F,Gershenzon J,Köllner TG

更新日期：2017-12-01 00:00:00

abstract：:A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...

journal_title：Plant molecular biology

authors： Ohmido N,Akiyama Y,Fukui K

更新日期：1998-12-01 00:00:00

abstract：:DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...

journal_title：Plant molecular biology

authors： Babinger P,Völkl R,Cakstina I,Maftei A,Schmitt R

更新日期：2007-02-01 00:00:00

abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

journal_title：Plant molecular biology

authors： Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

更新日期：1999-01-01 00:00:00

abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...

journal_title：Plant molecular biology

authors： Muñiz García MN,Muro MC,Mazzocchi LC,País SM,Stritzler M,Schlesinger M,Capiati DA

更新日期：2017-02-01 00:00:00

abstract：:WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...

journal_title：Plant molecular biology

authors： Zheng L,Liu G,Meng X,Liu Y,Ji X,Li Y,Nie X,Wang Y

更新日期：2013-07-01 00:00:00

abstract：:There was no discernible effect after incubating recombinant Anabaena Rubisco and carboxyarabinitol 1-phosphate with the product of the Anabaena rca gene. Since the unactivated cyanobacterial Rubisco is not readily inhibited by ribulose 1,5-bisphosphate and fallover is not observed, a genetic basis for the function of...

journal_title：Plant molecular biology

authors： Li LA,Zianni MR,Tabita FR

更新日期：1999-06-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...

journal_title：Plant molecular biology

authors： Chisholm D,Williams JG

更新日期：1988-07-01 00:00:00

abstract：:As derived from a cDNA clone, the structure of the b-32 protein of Zea mays, a putative regulatory factor of zein expression, has a central acidic region separated by two domains covered by secondary structure motifs. In this work, three b-32 genomic clones were selected from two genomic libraries obtained from the ma...

journal_title：Plant molecular biology

authors： Hartings H,Lazzaroni N,Marsan PA,Aragay A,Thompson R,Salamini F,Di Fonzo N,Palau J,Motto M

更新日期：1990-06-01 00:00:00

abstract：:Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...

journal_title：Plant molecular biology

authors： Griffor MC,Vodkin LO,Singh RJ,Hymowitz T

更新日期：1991-07-01 00:00:00

abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...

journal_title：Plant molecular biology

authors： Jensen MK,Rung JH,Gregersen PL,Gjetting T,Fuglsang AT,Hansen M,Joehnk N,Lyngkjaer MF,Collinge DB

更新日期：2007-09-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

journal_title：Plant molecular biology

authors： Carballo M,Giné R,Santos M,Puigdomènech P

更新日期：1991-01-01 00:00:00

abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...

journal_title：Plant molecular biology

authors： Sharma S,Holme IB,Dionisio G,Kodama M,Dzhanfezova T,Joernsgaard B,Brinch-Pedersen H

更新日期：2020-07-01 00:00:00

abstract：:Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...

journal_title：Plant molecular biology

authors： Cheng Z,Snustad DP,Carter JV

更新日期：2001-10-01 00:00:00

abstract：:The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...

journal_title：Plant molecular biology

authors： Urquhart W,Gunawardena AH,Moeder W,Ali R,Berkowitz GA,Yoshioka K

更新日期：2007-12-01 00:00:00

abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

journal_title：Plant molecular biology

authors： Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

更新日期：1994-01-01 00:00:00

abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

journal_title：Plant molecular biology

authors： Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

更新日期：2011-10-01 00:00:00

abstract：:A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-termin...

journal_title：Plant molecular biology

authors： Corr-Menguy F,Cejudo FJ,Mazubert C,Vidal J,Lelandais-Brière C,Torres G,Rode A,Hartmann C

更新日期：2002-11-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

journal_title：Plant molecular biology

authors： Luo L,Herrin DL

更新日期：2012-07-01 00:00:00

abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...

journal_title：Plant molecular biology

authors： Gaspar Y,Johnson KL,McKenna JA,Bacic A,Schultz CJ

更新日期：2001-09-01 00:00:00

abstract：KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...

journal_title：Plant molecular biology

authors： Liu X,Wu D,Shan T,Xu S,Qin R,Li H,Negm M,Wu D,Li J

更新日期：2020-07-01 00:00:00

abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

journal_title：Plant molecular biology

authors： Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

更新日期：2011-04-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...

journal_title：Plant molecular biology

authors： Gepstein S,Glick BR

更新日期：2013-08-01 00:00:00

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00