An improved chemically inducible gene switch that functions in the monocotyledonous plant sugar cane.

Abstract:

:Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene switches in plants because of its potential in both fundamental research and commercial biotechnology applications. However, there are no published reports demonstrating that this versatile gene switch is functional in transgenic monocotyledonous plants, which include some of the most important agricultural crops. We found that the original alc gene switch was ineffective in the monocotyledonous plant sugar cane, and describe a modified alc system that is functional in this globally significant crop. A promoter consisting of tandem copies of the ethanol receptor inverted repeat binding site, in combination with a minimal promoter sequence, was sufficient to give enhanced sensitivity and significantly higher levels of ethanol inducible gene expression. A longer CaMV 35S minimal promoter than was used in the original alc gene switch also substantially improved ethanol inducibility. Treating the roots with ethanol effectively induced the modified alc system in sugar cane leaves and stem, while an aerial spray was relatively ineffective. The extension of this chemically inducible gene expression system to sugar cane opens the door to new opportunities for basic research and crop biotechnology.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Kinkema M,Geijskes RJ,Shand K,Coleman HD,De Lucca PC,Palupe A,Harrison MD,Jepson I,Dale JL,Sainz MB

doi

10.1007/s11103-013-0140-2

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

443-54

issue

4-5

eissn

0167-4412

issn

1573-5028

journal_volume

84

pub_type

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