Abstract：
:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids. Its size and copy number in Escherichia coli offers increased efficiencies in routine in vitro recombination procedures. pGreen can replicate in Agrobacterium only if another plasmid, pSoup, is co-resident in the same strain. pSoup provides replication functions in trans for pGreen. The removal of RepA and Mob functions has enabled the size of pGreen to be kept to a minimum. Versions of pGreen have been used to transform several plant species with the same efficiencies as other binary Ti vectors. Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Hellens RP,Edwards EA,Leyland NR,Bean S,Mullineaux PMdoi
10.1023/a:1006496308160subject
Has Abstractpub_date
2000-04-01 00:00:00pages
819-32issue
6eissn
0167-4412issn
1573-5028journal_volume
42pub_type
杂志文章abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1016029001537
更新日期：2002-09-01 00:00:00
abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0581-5
更新日期：2017-04-01 00:00:00
abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021542
更新日期：1993-11-01 00:00:00
abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040674
更新日期：1992-02-01 00:00:00
abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0195-8
更新日期：2014-07-01 00:00:00
abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039423
更新日期：1990-12-01 00:00:00
abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00018558
更新日期：1990-02-01 00:00:00
abstract：:As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027403
更新日期：1988-05-01 00:00:00
abstract：:We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006038117130
更新日期：1998-12-01 00:00:00
abstract：:Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0351-2
更新日期：2005-10-01 00:00:00
abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015227
更新日期：1986-07-01 00:00:00
abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010614522706
更新日期：2001-07-01 00:00:00
abstract：:A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005728411921
更新日期：1997-02-01 00:00:00
abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027135
更新日期：1986-11-01 00:00:00
abstract：:In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021423
更新日期：1993-10-01 00:00:00
abstract：:Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3477-8
更新日期：2004-11-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00981-5
更新日期：2020-05-01 00:00:00
abstract：:The microtubular element of the plant cytoskeleton undergoes dramatic architectural changes in the course of the cell cycle, specifically at the entry into and exit from mitosis. These changes underlie the acquisition of specialized properties and functions involved, for example, in the equal segregation of chromosome...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006346107807
更新日期：2000-08-01 00:00:00
abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019126
更新日期：1995-10-01 00:00:00
abstract：:A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027164
更新日期：1992-12-01 00:00:00
abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005949304445
更新日期：1998-03-01 00:00:00
abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1011848528377
更新日期：2001-11-01 00:00:00
abstract：:The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021797
更新日期：1996-05-01 00:00:00
abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040585
更新日期：1994-01-01 00:00:00
abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0640-6
更新日期：2017-09-01 00:00:00
abstract：:Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00016474
更新日期：1994-12-01 00:00:00
abstract：:A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023388
更新日期：1992-06-01 00:00:00
abstract：:Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9281-5
更新日期：2008-03-01 00:00:00
abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1021162321527
更新日期：2003-01-01 00:00:00
