Abstract:
:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbouring this gene construct showed that the B3 promoter is expressed in leaves, stem, petioles and several flower tissues. The pattern of cell type-specific expression in stems and flowers was found to be developmentally regulated. Expression of the B3 promoter was found not to be heat-inducible, but highly repressed by wounding. The rapid decay in GUS activity upon wounding indicates that, at least under some physiological conditions, the gene product of this reporter gene is not as stable as has been previously thought.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella Ldoi
10.1007/BF00040585subject
Has Abstractpub_date
1994-01-01 00:00:00pages
195-202issue
1eissn
0167-4412issn
1573-5028journal_volume
24pub_type
杂志文章abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041174
更新日期:1995-11-01 00:00:00
abstract::Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015678
更新日期:1985-05-01 00:00:00
abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019103
更新日期:1996-11-01 00:00:00
abstract::When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020185
更新日期:1995-01-01 00:00:00
abstract::Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0054-3
更新日期:2006-08-01 00:00:00
abstract::The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0380-4
更新日期:2015-12-01 00:00:00
abstract::Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9125-8
更新日期:2007-04-01 00:00:00
abstract::The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006369104530
更新日期:1999-10-01 00:00:00
abstract::The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8883-z
更新日期:2005-09-01 00:00:00
abstract::We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-0954-7
更新日期:2005-03-01 00:00:00
abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040644
更新日期:1991-09-01 00:00:00
abstract::We have characterized a gene specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis. Differential screening led to the isolation of a floral nectary-specific cDNA clone. Northern hybridization indicated that its mRNA transcript is 1450 nucleotides long and specific to the flower base....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006381625421
更新日期:2000-03-01 00:00:00
abstract::The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020326
更新日期:1986-11-01 00:00:00
abstract::Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014876409166
更新日期:2002-03-01 00:00:00
abstract::Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017993
更新日期:1987-11-01 00:00:00
abstract::APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9711-7
更新日期:2011-01-01 00:00:00
abstract:KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00831-z
更新日期:2019-03-01 00:00:00
abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039617
更新日期:1993-01-01 00:00:00
abstract::R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0773-2
更新日期:2018-09-01 00:00:00
abstract::Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028854
更新日期:1994-11-01 00:00:00
abstract::Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027384
更新日期:1988-05-01 00:00:00
abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005959203396
更新日期:1997-12-01 00:00:00
abstract::Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028983
更新日期:1993-09-01 00:00:00
abstract::Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006496308160
更新日期:2000-04-01 00:00:00
abstract::Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006094015513
更新日期:1998-11-01 00:00:00
abstract::Many chloroplast proteins are synthesized in the cytoplasm as precursors which contain an amino terminal transit peptide. These precursors are subsequently imported into chloroplast and targeted to one of several organellar locations. This import is mediated by the transit peptide, which is cleaved off during import. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014912
更新日期:1987-07-01 00:00:00
abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-7307-4
更新日期:2005-07-01 00:00:00
abstract::We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9608-5
更新日期:2010-05-01 00:00:00
abstract::The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9344-2
更新日期:2008-08-01 00:00:00
abstract::A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00034956
更新日期:1992-01-01 00:00:00