The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin.

Abstract:

:A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5' untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Spence MJ,Henzl MT,Lammers PJ

doi

10.1007/BF00040644

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

499-504

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

17

pub_type

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