Abstract:
:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respectively. The N-terminals of deduced amino acid sequences of the two cDNAs showed typical features of the transit peptide of chloroplast targeting proteins. The ornithine-binding domain (FMHCLP) and catalytic domain (HPXQ) of ClOCT1 and ClOCT2 and the carbamoyl phosphate (CP)-binding site of ClOCT1 (SMRTR) are identical to OCTs of other plant species, pea and Arabidopsis thaliana. However, the CP-binding site sequence of ClOCT2, SLRTH, has not yet been reported. Both ClOCT1 and ClOCT2 cDNAs were expressed in Escherichia coli BL21 (DE3) by using expression vector pET30a. Recombinant ClOCT1 protein showed 14 times higher ornithine-dependent OCT activity than canaline-dependent OCT activity. In contrast, recombinant ClOCT2 protein showed 13 times higher canaline-dependent OCT activity than ornithine-dependent OCT activity. The two amino acids of the CP-binding site of ClOCT2 (SLRTH) were combinatorially changed to those of the CP-binding site of ClOCT1 (SMRTR) by site-directed mutagenesis. When Leu-118 of ClOCT2 was changed to Met, ornithine-dependent activity was increased significantly. It is assumed that the substrate specificity of ClOCT1 or ClOCT2 proteins partially depends on the amino acid sequence of the CP-binding site.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YMdoi
10.1023/a:1011632927541subject
Has Abstractpub_date
2001-08-01 00:00:00pages
651-60issue
6eissn
0167-4412issn
1573-5028journal_volume
46pub_type
杂志文章abstract::The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1993-08-01 00:00:00
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更新日期:1992-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014447
更新日期:1994-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037027
更新日期:1995-03-01 00:00:00
abstract:KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01064-1
更新日期:2020-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020122
更新日期:1996-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11103-010-9718-0
更新日期:2011-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-3095-0
更新日期:2005-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024116
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,已发布勘误
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更新日期:1993-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9585-8
更新日期:2010-03-01 00:00:00
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journal_title:Plant molecular biology
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doi:10.1007/s11103-010-9705-5
更新日期:2010-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1993-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006419232075
更新日期:2000-05-01 00:00:00
abstract::DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...
journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00