Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene.

Abstract:

:Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possible functions of these introns and to generate a mutant for simplified psbA gene manipulations, a psbA cDNA fragment was introduced into a psbA deletion mutant using the biolistic transformation method. A transformant with no introns in the psbA gene has been obtained and represents the first example of the removal of a complete set of introns from a chloroplast gene. The newly generated strain is photosynthetically competent and contains no detectable recipient genome copies. The loss of all four introns appears to be phenotypically silent.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Johanningmeier U,Heiss S

doi

10.1007/BF00038998

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

91-9

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

22

pub_type

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