The reconstruction and biochemical characterization of ancestral genes furnish insights into the evolution of terpene synthase function in the Poaceae.

Abstract:

KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as substrates and convert them into the various terpene skeletons by catalyzing a carbocation-driven reaction. Based on their sequence similarity, terpene synthases from land plants can be divided into different subfamilies, TPS-a to TPS-h. In this study, we aimed to understand the evolution and functional diversification of the TPS-a subfamily in the Poaceae (the grass family), a plant family that contains important crops such as maize, wheat, rice, and sorghum. Sequence comparisons showed that aside from one clade shared with other monocot plants, the Poaceae TPS-a subfamily consists of five well-defined clades I-V, the common ancestor of which probably originated very early in the evolution of the grasses. A survey of the TPS literature and the characterization of representative TPS enzymes from clades I-III revealed clade-specific substrate and product specificities. The enzymes in both clade I and II function as sesquiterpene synthases with clade I enzymes catalyzing initial C10-C1 or C11-C1 ring closures and clade II enzymes catalyzing C6-C1 closures. The enzymes of clade III mainly act as monoterpene synthases, forming cyclic and acyclic monoterpenes. The reconstruction and characterization of clade ancestors demonstrated that the differences among clades I-III were already present in their ancestors. However, the ancestors generally catalyzed simpler reactions with less double-bond isomerization and fewer cyclization steps. Overall, our data indicate an early origin of key enzymatic features of TPS-a enzymes in the Poaceae, and the development of more complex reactions over the course of evolution.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

doi

10.1007/s11103-020-01037-4

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

203-215

issue

1-2

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-020-01037-4

journal_volume

104

pub_type

杂志文章
  • Rice-specific Argonaute 17 controls reproductive growth and yield-associated phenotypes.

    abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01071-2

    authors: Pachamuthu K,Swetha C,Basu D,Das S,Singh I,Sundar VH,Sujith TN,Shivaprasad PV

    更新日期:2021-01-01 00:00:00

  • Analysis of physiological and miRNA responses to Pi deficiency in alfalfa (Medicago sativa L.).

    abstract:KEY MESSAGE:The induction of miR399 and miR398 and the inhibition of miR156, miR159, miR160, miR171, miR2111, and miR2643 were observed under Pi deficiency in alfalfa. The miRNA-mediated genes involved in basic metabolic process, root and shoot development, stress response and Pi uptake. Inorganic phosphate (Pi) defici...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0711-3

    authors: Li Z,Xu H,Li Y,Wan X,Ma Z,Cao J,Li Z,He F,Wang Y,Wan L,Tong Z,Li X

    更新日期:2018-03-01 00:00:00

  • Analysis of regulatory elements involved in stress-induced and organ-specific expression of tobacco acidic and basic beta-1,3-glucanase genes.

    abstract::Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028803

    authors: van de Rhee MD,Lemmers R,Bol JF

    更新日期:1993-02-01 00:00:00

  • Rejuvenation by shoot apex culture recapitulates the developmental increase of methylation at the maize gene Pl-Blotched.

    abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-5620-6

    authors: Irish EE,McMurray D

    更新日期:2006-03-01 00:00:00

  • Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts.

    abstract::cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040617

    authors: Salter AH,Newman BJ,Napier JA,Gray JC

    更新日期:1992-11-01 00:00:00

  • CYCLIN-DEPENDENT KINASE G2 regulates salinity stress response and salt mediated flowering in Arabidopsis thaliana.

    abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0324-z

    authors: Ma X,Qiao Z,Chen D,Yang W,Zhou R,Zhang W,Wang M

    更新日期:2015-06-01 00:00:00

  • Ectopic expression of UGT75D1, a glycosyltransferase preferring indole-3-butyric acid, modulates cotyledon development and stress tolerance in seed germination of Arabidopsis thaliana.

    abstract::The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0395-x

    authors: Zhang GZ,Jin SH,Jiang XY,Dong RR,Li P,Li YJ,Hou BK

    更新日期:2016-01-01 00:00:00

  • RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species.

    abstract::A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9031-0

    authors: Luo H,Lee JY,Hu Q,Nelson-Vasilchik K,Eitas TK,Lickwar C,Kausch AP,Chandlee JM,Hodges TK

    更新日期:2006-10-01 00:00:00

  • Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants.

    abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-5817-8

    authors: Ueda H,Yamaguchi Y,Sano H

    更新日期:2006-05-01 00:00:00

  • Temporal and tissue-specific expression of the tobacco ntf4 MAP kinase.

    abstract::The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010645431133

    authors: Voronin V,Touraev A,Kieft H,van Lammeren AA,Heberle-Bors E,Wilson C

    更新日期:2001-04-01 00:00:00

  • CR1 - a dispersed repeated element associated with the Cab-1 locus in tomato.

    abstract::Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014948

    authors: Bernatzky R,Pichersky E,Malik VS,Tanksley SD

    更新日期:1988-09-01 00:00:00

  • An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.

    abstract::Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040638

    authors: Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L

    更新日期:1991-09-01 00:00:00

  • A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity.

    abstract::The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040578

    authors: Kim Y,Buckley K,Costa MA,An G

    更新日期:1994-01-01 00:00:00

  • The Arabidopsis PPDK gene is transcribed from two promoters to produce differentially expressed transcripts responsible for cytosolic and plastidic proteins.

    abstract::Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9023-0

    authors: Parsley K,Hibberd JM

    更新日期:2006-10-01 00:00:00

  • Enhancing disease resistance in poplar through modification of its natural defense pathway.

    abstract:KEY MESSAGE:Modification of the poplar defense pathway through pathogen-induced expression of an amphibian host defense peptide modulates plant innate immunity and confers robust and reliable resistance against a major poplar pathogen, Septoria musiva. Host defense peptides (HDPs), also known as cationic antimicrobial ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00874-2

    authors: Yevtushenko DP,Misra S

    更新日期:2019-07-01 00:00:00

  • A tandem of alpha-tubulin genes preferentially expressed in radicular tissues from Zea mays.

    abstract::The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015650

    authors: Montoliu L,Rigau J,Puigdomènech P

    更新日期:1990-01-01 00:00:00

  • FRIGIDA and related proteins have a conserved central domain and family specific N- and C- terminal regions that are functionally important.

    abstract::Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9635-2

    authors: Risk JM,Laurie RE,Macknight RC,Day CL

    更新日期:2010-07-01 00:00:00

  • Potato granule-bound starch synthase promoter-controlled GUS expression: regulation of expression after transient and stable transformation.

    abstract::Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00029145

    authors: van der Steege G,Nieboer M,Swaving J,Tempelaar MJ

    更新日期:1992-10-01 00:00:00

  • Specific interaction of the tomato bZIP transcription factor VSF-1 with a non-palindromic DNA sequence that controls vascular gene expression.

    abstract::The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006030007333

    authors: Ringli C,Keller B

    更新日期:1998-08-01 00:00:00

  • The cruciferin gene family in radish.

    abstract::In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three dis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040606

    authors: Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

    更新日期:1992-11-01 00:00:00

  • Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions.

    abstract::Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000009296.91149.34

    authors: Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

    更新日期:2003-09-01 00:00:00

  • Transgene silencing of invertedly repeated transgenes is released upon deletion of one of the transgenes involved.

    abstract::To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010614522706

    authors: De Buck S,Van Montagu M,Depicker A

    更新日期:2001-07-01 00:00:00

  • Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte.

    abstract::A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006282507095

    authors: Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

    更新日期:1999-07-01 00:00:00

  • Transgene-mediated auxin overproduction in Arabidopsis: hypocotyl elongation phenotype and interactions with the hy6-1 hypocotyl elongation and axr1 auxin-resistant mutants.

    abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020881

    authors: Romano CP,Robson PR,Smith H,Estelle M,Klee H

    更新日期:1995-03-01 00:00:00

  • The maize mitochondrial plasmid RNA b is associated with protein during synthesis but is not encapsidated.

    abstract::RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005818631401

    authors: Formanová N,Brown GG

    更新日期:1997-06-01 00:00:00

  • Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasms.

    abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF02418241

    authors: Abbott AG,O'Dell M,Flavell RB

    更新日期:1985-07-01 00:00:00

  • Expression of the pea (Pisum sativum L.) alpha-tubulin gene TubA1 is correlated with cell division activity.

    abstract::Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006338401808

    authors: Stotz HU,Long SR

    更新日期:1999-11-01 00:00:00

  • Lignin synthesized by CmCAD2 and CmCAD3 in oriental melon (Cucumis melo L.) seedlings contributes to drought tolerance.

    abstract:KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01018-7

    authors: Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

    更新日期:2020-08-01 00:00:00

  • The Arabidopsis chloroplast RNase J displays both exo- and robust endonucleolytic activities.

    abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0799-5

    authors: Halpert M,Liveanu V,Glaser F,Schuster G

    更新日期:2019-01-01 00:00:00

  • Distinct reorganization of the genome transcription associates with organogenesis of somatic embryo, shoots, and roots in rice.

    abstract::Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9092-0

    authors: Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

    更新日期:2007-02-01 00:00:00