Abstract:
:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The KP4 toxin expressed in this transgenic plant was of the same size and specificity as the authentic Ustilago KP4 toxin. The expression level was at least 500 times higher than that of the KP6 toxin expressed in plants. Transgenic crop plants producing the KP4 toxin could be rendered resistant to KP4-susceptible fungal pathogens.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Park CM,Berry JO,Bruenn JAdoi
10.1007/BF00020122subject
Has Abstractpub_date
1996-01-01 00:00:00pages
359-66issue
2eissn
0167-4412issn
1573-5028journal_volume
30pub_type
杂志文章abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020881
更新日期:1995-03-01 00:00:00
abstract::We have isolated and characterized a cDNA from a strawberry fruit subtractive library that shows homology to class-I low-molecular-weight (LMW) heat-shock protein genes from other higher plants. The strawberry cDNA (clone njjs4) was a 779 bp full-length cDNA with a single open reading frame of 468 bp that is expected ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005994800671
更新日期:1998-01-01 00:00:00
abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1015500431421
更新日期:2002-08-01 00:00:00
abstract:KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0542-z
更新日期:2016-12-01 00:00:00
abstract::In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006438321386
更新日期:2000-08-01 00:00:00
abstract::A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017808
更新日期:1996-01-01 00:00:00
abstract::Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9071-5
更新日期:2007-01-01 00:00:00
abstract::Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019312
更新日期:1995-02-01 00:00:00
abstract::Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006133311402
更新日期:1999-04-01 00:00:00
abstract::Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9752-6
更新日期:2011-04-01 00:00:00
abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1005816823636
更新日期:1997-12-01 00:00:00
abstract::An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006129615846
更新日期:1999-03-01 00:00:00
abstract::We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006038117130
更新日期:1998-12-01 00:00:00
abstract::We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010686931889
更新日期:2001-07-01 00:00:00
abstract::The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023618
更新日期:1993-03-01 00:00:00
abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014183
更新日期:1987-01-01 00:00:00
abstract::Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0140-2
更新日期:2014-03-01 00:00:00
abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0555-7
更新日期:2017-02-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9281-5
更新日期:2008-03-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1021162321527
更新日期:2003-01-01 00:00:00
abstract:KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0684-7
更新日期:2018-02-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0320-3
更新日期:2015-06-01 00:00:00
abstract::Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039627
更新日期:1993-01-01 00:00:00
abstract::In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028867
更新日期:1994-11-01 00:00:00
abstract::Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3474-y
更新日期:2004-10-01 00:00:00
abstract::The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021430
更新日期:1993-10-01 00:00:00
abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043873
更新日期:1994-06-01 00:00:00
abstract::Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036810
更新日期:1991-07-01 00:00:00
abstract::Darjeeling teas are the highest grown teas in the world and preferred for its flavour, aroma and quality. Apart from the genetic makeup of the plant, earlier reports suggest that insect infestation, particularly jassids and thrips triggers the aroma and flavour formation in Darjeeling tea. The present work encompasses...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9887-0
更新日期:2012-04-01 00:00:00