Abstract:
:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at2S2 gene, protein levels were much lower.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Conceição Ada S,Van Vliet A,Krebbers Edoi
10.1007/BF00028867subject
Has Abstractpub_date
1994-11-01 00:00:00pages
1001-5issue
3eissn
0167-4412issn
1573-5028journal_volume
26pub_type
杂志文章abstract::The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020616
更新日期:1996-04-01 00:00:00
abstract::Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9297-5
更新日期:2008-04-01 00:00:00
abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5620-6
更新日期:2006-03-01 00:00:00
abstract::The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0317-y
更新日期:2015-06-01 00:00:00
abstract::Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-1692-y
更新日期:2004-07-01 00:00:00
abstract::The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-2525-8
更新日期:2004-09-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020881
更新日期:1995-03-01 00:00:00
abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015971
更新日期:1993-06-01 00:00:00
abstract::Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0445-z
更新日期:2016-05-01 00:00:00
abstract::Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9984-0
更新日期:2013-01-01 00:00:00
abstract::The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015643
更新日期:1987-03-01 00:00:00
abstract:KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01037-4
更新日期:2020-09-01 00:00:00
abstract::Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9655-y
更新日期:2010-09-01 00:00:00
abstract::A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020195
更新日期:1995-01-01 00:00:00
abstract::Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9380-y
更新日期:2009-03-01 00:00:00
abstract:KEY MESSAGE:The expression of MdBBX10 was significantly induced by different stresses and ABA treatments. Overexpression of MdBBX10 in Arabidopsis significantly enhanced abiotic stresses tolerance by ABA signalling. The roles of B-box domain(s) containing proteins (BBXs) in regulation of flowering and light morphogenes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00828-8
更新日期:2019-03-01 00:00:00
abstract::StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9462-5
更新日期:2009-05-01 00:00:00
abstract::The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010665831955
更新日期:2001-05-01 00:00:00
abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022324920316
更新日期:2003-03-01 00:00:00
abstract::Arabidopsis is currently the reference genome for higher plants. A new, more detailed statistical analysis of Arabidopsis gene structure is presented including intron and exon lengths, intergenic distances, features of promoters, and variant 5'-ends of mRNAs transcribed from the same transcription unit. We also provid...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2564-9
更新日期:2006-01-01 00:00:00
abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9698-0
更新日期:2010-12-01 00:00:00
abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039617
更新日期:1993-01-01 00:00:00
abstract::To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040585
更新日期:1994-01-01 00:00:00
abstract:KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0808-8
更新日期:2019-01-01 00:00:00
abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5101-y
更新日期:2005-06-01 00:00:00
abstract::The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011632927541
更新日期:2001-08-01 00:00:00
abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020199
更新日期:1996-11-01 00:00:00
abstract::The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024116
更新日期:1994-02-01 00:00:00
abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028878
更新日期:1994-08-01 00:00:00
abstract::In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005736722794
更新日期:1997-04-01 00:00:00