Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

Abstract:

:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at2S2 gene, protein levels were much lower.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Conceição Ada S,Van Vliet A,Krebbers E

doi

10.1007/BF00028867

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

1001-5

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

26

pub_type

杂志文章
  • Characterisation of a wound-induced transcript from the monocot asparagus that shares similarity with a class of intracellular pathogenesis-related (PR) proteins.

    abstract::We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00026782

    authors: Warner SA,Scott R,Draper J

    更新日期:1992-07-01 00:00:00

  • Nucleotide sequence of psbC, the gene encoding the CP-43 chlorophyll a-binding protein of Photosystem II, in the cyanobacterium Synechocystis 6803.

    abstract::The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00029879

    authors: Chisholm D,Williams JG

    更新日期:1988-07-01 00:00:00

  • A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.

    abstract::Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9926-x

    authors: Luo L,Herrin DL

    更新日期:2012-07-01 00:00:00

  • New insights into Oryza genome evolution: high gene colinearity and differential retrotransposon amplification.

    abstract::An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-007-9178-3

    authors: Zhang S,Gu YQ,Singh J,Coleman-Derr D,Brar DS,Jiang N,Lemaux PG

    更新日期:2007-07-01 00:00:00

  • Protein import into cyanelles and complex chloroplasts.

    abstract::Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:

    authors: Schwartzbach SD,Osafune T,Löffelhardt W

    更新日期:1998-09-01 00:00:00

  • Lysine-rich modified gamma-zeins accumulate in protein bodies of transiently transformed maize endosperms.

    abstract::During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005889314967

    authors: Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

    更新日期:1997-05-01 00:00:00

  • Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

    abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005909703600

    authors: Atanassova R,Flénet M,Gigot C,Chaubet N

    更新日期:1998-05-01 00:00:00

  • In silico analysis of the sequence features responsible for alternatively spliced introns in the model green alga Chlamydomonas reinhardtii.

    abstract::Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0605-9

    authors: Raj-Kumar PK,Vallon O,Liang C

    更新日期:2017-06-01 00:00:00

  • Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts.

    abstract::We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027118

    authors: Rathore KS,Chowdhury VK,Hodges TK

    更新日期:1993-03-01 00:00:00

  • Isolation of a novel Arabidopsis ozone-induced cDNA by differential display.

    abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019121

    authors: Sharma YK,Davis KR

    更新日期:1995-10-01 00:00:00

  • Constitutive expression of the beta-phaseolin gene in different tissues of transgenic alfalfa does not ensure phaseolin accumulation in non-seed tissue.

    abstract::Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040527

    authors: Bagga S,Sutton D,Kemp JD,Sengupta-Gopalan C

    更新日期:1992-09-01 00:00:00

  • Senescence-induced expression of a homologue of delta 9 desaturase in rose petals.

    abstract::cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041154

    authors: Fukuchi-Mizutani M,Savin K,Cornish E,Tanaka Y,Ashikari T,Kusumi T,Murata N

    更新日期:1995-11-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.). 8. Cis-elements and trans-acting factors for the quantitative expression of a bean chalcone synthase gene promoter in electroporated alfalfa protoplasts.

    abstract::A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015079

    authors: Harrison MJ,Choudhary AD,Dubery I,Lamb CJ,Dixon RA

    更新日期:1991-05-01 00:00:00

  • The region distal to the rRNA operon from chloroplasts of maize contains genes coding for tRNA(Arg)(ACG) and tRNA (Asn)(GUU).

    abstract::The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020326

    authors: Dormann-Przybyl D,Strittmatter G,Kössel H

    更新日期:1986-11-01 00:00:00

  • Contrasting evolutionary patterns and target specificities among three Tourist-like MITE families in the maize genome.

    abstract::Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9511-0

    authors: Zerjal T,Joets J,Alix K,Grandbastien MA,Tenaillon MI

    更新日期:2009-09-01 00:00:00

  • OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.

    abstract::Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9752-6

    authors: Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

    更新日期:2011-04-01 00:00:00

  • miR164-targeted TaPSK5 encodes a phytosulfokine precursor that regulates root growth and yield traits in common wheat (Triticum aestivum L.).

    abstract:KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01064-1

    authors: Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

    更新日期:2020-12-01 00:00:00

  • Ordered assembly of mitochondria during rice germination begins with pro-mitochondrial structures rich in components of the protein import apparatus.

    abstract::Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-3688-7

    authors: Howell KA,Millar AH,Whelan J

    更新日期:2006-01-01 00:00:00

  • Molecular characterization and expression study of a histidine auxotrophic mutant (his1-) of Nicotiana plumbaginifolia.

    abstract::The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006493021557

    authors: El Malki F,Jacobs M

    更新日期:2001-01-01 00:00:00

  • CYP71Z18 overexpression confers elevated blast resistance in transgenic rice.

    abstract:KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00881-3

    authors: Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

    更新日期:2019-08-01 00:00:00

  • Specificity of auxin regulation of gibberellin 20-oxidase gene expression in pea pericarp.

    abstract::During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015522404586

    authors: Ngo P,Ozga JA,Reinecke DM

    更新日期:2002-07-01 00:00:00

  • OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice.

    abstract::The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0115-3

    authors: Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

    更新日期:2014-01-01 00:00:00

  • Paramutation in maize.

    abstract::Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006499808317

    authors: Chandler VL,Eggleston WB,Dorweiler JE

    更新日期:2000-06-01 00:00:00

  • A platform for soybean molecular breeding: the utilization of core collections for food security.

    abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-013-0076-6

    authors: Qiu LJ,Xing LL,Guo Y,Wang J,Jackson SA,Chang RZ

    更新日期:2013-09-01 00:00:00

  • Differential ethylene-inducible expression of cellulase in pepper plants.

    abstract::Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041164

    authors: Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

    更新日期:1995-11-01 00:00:00

  • Downstream DNA sequences are required to modulate Pvlea-18 gene expression in response to dehydration.

    abstract::We have previously shown that mRNA and protein encoded by the Pvlea-18 gene from Phaseolus vulgaris L., a member of a new family of late embryogenesis-abundant (LEA) proteins, accumulate in dark-grown bean seedlings not only in response to water deficit but also during optimal irrigation. In this work, we studied Pvle...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010607223533

    authors: Moreno-Fonseca LP,Covarrubias AA

    更新日期:2001-03-01 00:00:00

  • Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thaliana and is targeted to the chloroplasts.

    abstract::Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028854

    authors: Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

    更新日期:1994-11-01 00:00:00

  • Two ftsH-family genes encoded in the nuclear and chloroplast genomes of the primitive red alga Cyanidioschyzon merolae.

    abstract::The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006369104530

    authors: Itoh R,Takano H,Ohta N,Miyagishima S,Kuroiwa H,Kuroiwa T

    更新日期:1999-10-01 00:00:00

  • OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.

    abstract::Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9500-3

    authors: Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK

    更新日期:2009-08-01 00:00:00

  • A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities.

    abstract::An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006129615846

    authors: Lopato S,Gattoni R,Fabini G,Stevenin J,Barta A

    更新日期:1999-03-01 00:00:00