Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

Abstract:

:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at2S2 gene, protein levels were much lower.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Conceição Ada S,Van Vliet A,Krebbers E

doi

10.1007/BF00028867

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

1001-5

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

26

pub_type

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