Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7.


KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wild-type (Col-0) and transgenic lines overexpressing PIP2;7 were used to investigate and compare their response to salt stress. Hydraulic conductivity measurements using a high-pressure flowmeter (HPFM) revealed that overexpression of PIP2;7 induced a sixfold increase in root hydraulic conductivity of four week-old Arabidopsis thaliana plants compared to WT. Exposure to a high salt stress (150 mM NaCl) triggered a rapid repression of overall aquaporin activity in both genotypes. Response to salt stress was also investigated in 8 day-old seedlings. Exposure to salt led to a repression of PIP2;7 promoter activity and a significant decrease in PIP2;7 mRNA abundance within 2 h. Concomitantly, a rapid internalization of fluorescently-tagged PIP2;7 proteins was observed but removal from the cell membrane was not accompanied by further degradation of the protein within 4 h of exposure to salinity stress. These data suggest that PIP transcriptional repression and channel internalization act in concert during salt stress conditions to modulate aquaporin activity, thereby significantly altering the plant hydraulic parameters in the short term.


Plant Mol Biol


Plant molecular biology


Pou A,Jeanguenin L,Milhiet T,Batoko H,Chaumont F,Hachez C




Has Abstract


2016-12-01 00:00:00














  • Eucalypt alpha-tubulin: cDNA cloning and increased level of transcripts in ectomycorrhizal root system.

    abstract::Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Diaz EC,Martin F,Tagu D

    更新日期:1996-07-01 00:00:00

  • New potential markers of in vitro tomato morphogenesis identified by mRNA differential display.

    abstract::The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

    更新日期:1996-12-01 00:00:00

  • Mesophyll-specific, light and metabolic regulation of the C4 PPCZm1 promoter in transgenic maize.

    abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kausch AP,Owen TP Jr,Zachwieja SJ,Flynn AR,Sheen J

    更新日期:2001-01-01 00:00:00

  • Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.

    abstract::Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev A

    更新日期:2007-01-01 00:00:00

  • The molecular characterization of transport vesicles.

    abstract::Secretion, endocytosis and transport to the lytic compartment are fundamental, highly coordinated features of the eukaryotic cell. These intracellular transport processes are facilitated by vesicles, many of which are small (100 nm or less in diameter) and 'coated' on their cytoplasmic surface. Research into the struc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Robinson DG,Hinz G,Holstein SE

    更新日期:1998-09-01 00:00:00

  • Correction to: Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.

    abstract::Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

    journal_title:Plant molecular biology

    pub_type: 已发布勘误


    authors: Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

    更新日期:2020-03-01 00:00:00

  • Structure and characterization of a putative drought-inducible H1 histone gene.

    abstract::A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Wei T,O'Connell MA

    更新日期:1996-01-01 00:00:00

  • Tissue-specific expression of two genes for sucrose synthase in carrot (Daucus carota L.).

    abstract::Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sturm A,Lienhard S,Schatt S,Hardegger M

    更新日期:1999-01-01 00:00:00

  • The Arabidopsis chloroplast RNase J displays both exo- and robust endonucleolytic activities.

    abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Halpert M,Liveanu V,Glaser F,Schuster G

    更新日期:2019-01-01 00:00:00

  • Organization of the major latex protein gene family in opium poppy.

    abstract::Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Nessler CL,Burnett RJ

    更新日期:1992-11-01 00:00:00

  • Strategies to ameliorate abiotic stress-induced plant senescence.

    abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Gepstein S,Glick BR

    更新日期:2013-08-01 00:00:00

  • CYCLIN-DEPENDENT KINASE G2 regulates salinity stress response and salt mediated flowering in Arabidopsis thaliana.

    abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ma X,Qiao Z,Chen D,Yang W,Zhou R,Zhang W,Wang M

    更新日期:2015-06-01 00:00:00

  • Unusual inheritance of evolutionarily-related double-stranded RNAs in interspecific hybrid between rice plants Oryza sativa and Oryza rufipogon.

    abstract::Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Moriyama H,Horiuchi H,Nitta T,Fukuhara T

    更新日期:1999-04-01 00:00:00

  • RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species.

    abstract::A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Luo H,Lee JY,Hu Q,Nelson-Vasilchik K,Eitas TK,Lickwar C,Kausch AP,Chandlee JM,Hodges TK

    更新日期:2006-10-01 00:00:00

  • Transposable element contributions to plant gene and genome evolution.

    abstract::Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Bennetzen JL

    更新日期:2000-01-01 00:00:00

  • An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana.

    abstract::Aquaporin belongs to a highly conserved group of membrane proteins called major intrinsic proteins that facilitate water transport across biological membranes. The genome of Arabidopsis encodes 35 aquaporin genes with 13 homologs in the plasma membrane intrinsic protein (PIP) subgroup. However, the function of each in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Jang JY,Kim DG,Kim YO,Kim JS,Kang H

    更新日期:2004-03-01 00:00:00

  • Genomic organization and evolution of the soybean SB92 satellite sequence.

    abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Vahedian M,Shi L,Zhu T,Okimoto R,Danna K,Keim P

    更新日期:1995-11-01 00:00:00

  • OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.

    abstract::Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK

    更新日期:2009-08-01 00:00:00

  • Identification of tomato Lhc promoter regions necessary for circadian expression.

    abstract::Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Piechulla B,Merforth N,Rudolph B

    更新日期:1998-11-01 00:00:00

  • Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogen

    abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

    更新日期:1998-02-01 00:00:00

  • Changes in translatable poly(A) RNA from differentiated potato tissues transformed with shoot-inducing Ti TL-DNA of Agrobacterium tumefaciens.

    abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Burrell MM,Temple S,Ooms G

    更新日期:1986-07-01 00:00:00

  • Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class.

    abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Roche D,Temple SJ,Sengupta-Gopalan C

    更新日期:1993-09-01 00:00:00

  • Vir-115 gene product is required to stabilize D1 translation intermediates in chloroplasts.

    abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kim J,Klein PG,Mullet JE

    更新日期:1994-06-01 00:00:00

  • Non-photosynthetic 'malic enzyme' from maize: a constituvely expressed enzyme that responds to plant defence inducers.

    abstract::The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Maurino VG,Saigo M,Andreo CS,Drincovich MF

    更新日期:2001-03-01 00:00:00

  • The rice OsDIL gene plays a role in drought tolerance at vegetative and reproductive stages.

    abstract::Drought is one of the critical factors limiting reproductive yields of rice and other crops globally. However, little is known about the molecular mechanism underlying reproductive development under drought stress in rice. To explore the potential gene function for improving rice reproductive development under drought...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Guo C,Ge X,Ma H

    更新日期:2013-06-01 00:00:00

  • Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat.

    abstract:KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Casartelli A,Melino VJ,Baumann U,Riboni M,Suchecki R,Jayasinghe NS,Mendis H,Watanabe M,Erban A,Zuther E,Hoefgen R,Roessner U,Okamoto M,Heuer S

    更新日期:2019-03-01 00:00:00

  • Alternative splicing is required for RCT1-mediated disease resistance in Medicago truncatula.

    abstract::RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Tang F,Yang S,Gao M,Zhu H

    更新日期:2013-07-01 00:00:00

  • The complex structures of arabinogalactan-proteins and the journey towards understanding function.

    abstract::Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Gaspar Y,Johnson KL,McKenna JA,Bacic A,Schultz CJ

    更新日期:2001-09-01 00:00:00

  • Genomic and functional characterization of StCDPK1.

    abstract::StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Gargantini PR,Giammaria V,Grandellis C,Feingold SE,Maldonado S,Ulloa RM

    更新日期:2009-05-01 00:00:00

  • Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA.

    abstract::The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

    更新日期:1984-07-01 00:00:00