Abstract：
:Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in elongation rates is the extensibility of the elongating cell walls. To help decipher the complex molecular mechanisms involved in spatially variable root growth, we performed a gene identification study along primary root tips of maize (Zea mays) seedlings using suppression subtractive hybridization (SSH) and candidate gene approaches. Using SSH we isolated 150 non-redundant cDNA clones representing root growth-related genes (RGGs) that were preferentially expressed in the elongation zone. Differential expression patterns were revealed by Northern blot analysis for 41 of the identified genes and several candidate genes. Many of the genes have not been previously reported to be involved in root growth processes in maize. Genes were classified into groups based on the predicted function of the encoded proteins: cell wall metabolism, cytoskeleton, general metabolism, signaling and unknown. In-situ hybridization performed for two selected genes, confirmed the spatial distribution of expression shown by Northern blots and revealed subtle differences in tissue localization. Interestingly, spatial profiles of expression for some cell wall related genes appeared to correlate with the profile of accelerating root elongation and changed appropriately under growth-inhibitory water deficit.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bassani M,Neumann PM,Gepstein Sdoi
10.1007/s11103-004-3474-ysubject
Has Abstractpub_date
2004-10-01 00:00:00pages
367-80issue
3eissn
0167-4412issn
1573-5028pii
DO00003474journal_volume
56pub_type
杂志文章abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9585-8
更新日期：2010-03-01 00:00:00
abstract：:Plants, algae, cyanobacteria and many other bacteria synthesize the tetrapyrrole precursor, delta-aminolevulinic acid (ALA), from glutamate by means of a tRNAGlu-mediated pathway. The enzyme glutamyl-tRNA reductase (GTR) catalyzes the first committed step in this pathway, which is the reduction of tRNA-bound glutamate...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-6803-x
更新日期：2005-07-01 00:00:00
abstract：:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019948
更新日期：1993-01-01 00:00:00
abstract：:Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9023-0
更新日期：2006-10-01 00:00:00
abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014544
更新日期：1993-02-01 00:00:00
abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-4679-9
更新日期：2004-11-01 00:00:00
abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9984-0
更新日期：2013-01-01 00:00:00
abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006030007333
更新日期：1998-08-01 00:00:00
abstract：:Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006499808317
更新日期：2000-06-01 00:00:00
abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020195
更新日期：1995-01-01 00:00:00
abstract：:DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9721-5
更新日期：2011-02-01 00:00:00
abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006148815106
更新日期：1999-02-01 00:00:00
abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006163030646
更新日期：1999-01-01 00:00:00
abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2525-8
更新日期：2004-09-01 00:00:00
abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020982
更新日期：1995-11-01 00:00:00
abstract：:A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015983
更新日期：1993-06-01 00:00:00
abstract：:Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019191
更新日期：1995-01-01 00:00:00
abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0233-6
更新日期：2014-11-01 00:00:00
abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005833216479
更新日期：1997-10-01 00:00:00
abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015227
更新日期：1986-07-01 00:00:00
abstract：:A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023430
更新日期：1991-04-01 00:00:00
abstract：:Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9448-8
更新日期：2009-04-01 00:00:00
abstract：:A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005897921567
更新日期：1997-12-01 00:00:00
abstract：:A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these tra...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040703
更新日期：1994-11-01 00:00:00
abstract：:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0082-8
更新日期：2013-10-01 00:00:00
abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023238
更新日期：1994-05-01 00:00:00
abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00187572
更新日期：1983-01-01 00:00:00
abstract：:Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005729309569
更新日期：1997-03-01 00:00:00
abstract：:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0459-6
更新日期：2016-06-01 00:00:00
abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027135
更新日期：1986-11-01 00:00:00
