Specific in vitro transcription of 16S rRNA gene by pea chloroplast RNA polymerase.

abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

journal_title：Plant molecular biology

authors： Leah R,Mundy J

更新日期：1989-06-01 00:00:00

abstract：:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...

journal_title：Plant molecular biology

authors： Helentjaris T,King G,Slocum M,Siedenstrang C,Wegman S

更新日期：1985-03-01 00:00:00

abstract：:Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...

journal_title：Plant molecular biology

authors： Dresselhaus T,Cordts S,Lörz H

更新日期：1999-03-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...

journal_title：Plant molecular biology

authors： Muñiz García MN,Muro MC,Mazzocchi LC,País SM,Stritzler M,Schlesinger M,Capiati DA

更新日期：2017-02-01 00:00:00

abstract：KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...

journal_title：Plant molecular biology

authors： Hildebrandt TM

更新日期：2018-09-01 00:00:00

abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

journal_title：Plant molecular biology

authors： Abbott AG,O'Dell M,Flavell RB

更新日期：1985-07-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...

journal_title：Plant molecular biology

authors： Hong Z,Geisler-Lee CJ,Zhang Z,Verma DP

更新日期：2003-10-01 00:00:00

abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

journal_title：Plant molecular biology

authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

更新日期：2003-02-01 00:00:00

abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

journal_title：Plant molecular biology

authors： Altschuler M,Mascarenhas JP

更新日期：1985-09-01 00:00:00

abstract：:Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...

journal_title：Plant molecular biology

authors： Daley DO,Considine MJ,Howell KA,Millar AH,Day DA,Whelan J

更新日期：2003-03-01 00:00:00

abstract：:The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...

journal_title：Plant molecular biology

authors： Hong SY,Lee TS,Kim J,Jung JH,Choi CW,Kim TG,Kwon TH,Jang YS,Yang MS

更新日期：2008-11-01 00:00:00

abstract：:Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...

journal_title：Plant molecular biology

authors： Mohamed A,Jansson C

更新日期：1991-05-01 00:00:00

abstract：:In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...

journal_title：Plant molecular biology

authors： Hirschi KD,Miranda ML,Wilganowski NL

更新日期：2001-05-01 00:00:00

abstract：:We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...

journal_title：Plant molecular biology

authors： Kurkela S,Franck M

更新日期：1990-07-01 00:00:00

abstract：:DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus ...

journal_title：Plant molecular biology

authors： Hess WR,Weihe A,Loiseaux-de Goër S,Partensky F,Vaulot D

更新日期：1995-03-01 00:00:00

abstract：KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

journal_title：Plant molecular biology

authors： McNeilly D,Schofield A,Stone SL

更新日期：2018-02-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...

journal_title：Plant molecular biology

authors： Prassinos C,Haralampidis K,Milioni D,Samakovli D,Krambis K,Hatzopoulos P

更新日期：2008-07-01 00:00:00

abstract：:The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...

journal_title：Plant molecular biology

authors： Ohtsubo N,Nakayama T,Terada R,Shimamoto K,Iwabuchi M

更新日期：1993-11-01 00:00:00

abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

journal_title：Plant molecular biology

authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

更新日期：1994-11-01 00:00:00

abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

journal_title：Plant molecular biology

authors： Xue Y,Collin S,Davies DR,Thomas CM

更新日期：1994-04-01 00:00:00

abstract：:Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...

journal_title：Plant molecular biology

authors： Yang P,Taoka K,Nakayama T,Iwabuchi M

更新日期：1995-04-01 00:00:00

abstract：:A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...

journal_title：Plant molecular biology

authors： Raines CA,Lloyd JC,Chao SM,John UP,Murphy GJ

更新日期：1991-04-01 00:00:00

abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...

journal_title：Plant molecular biology

authors： Laxa M

更新日期：2017-01-01 00:00:00

abstract：:The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...

journal_title：Plant molecular biology

authors： de Pater S,Pham K,Klitsie I,Kijne J

更新日期：1996-11-01 00:00:00

abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

journal_title：Plant molecular biology

authors： Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

更新日期：2011-10-01 00:00:00

abstract：:Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...

journal_title：Plant molecular biology

authors： Risk JM,Laurie RE,Macknight RC,Day CL

更新日期：2010-07-01 00:00:00

abstract：KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...

journal_title：Plant molecular biology

authors： Okada M,Yoshida K,Takumi S

更新日期：2017-12-01 00:00:00

abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

journal_title：Plant molecular biology

authors： Rose AB,Carter A,Korf I,Kojima N

更新日期：2016-10-01 00:00:00