Abstract：
:Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from day 10 to 15. Protein abundance followed transcript abundance for all components examined with the exception of the F1beta subunit of ATP synthase. A dramatic peak in F1beta transcript levels early in development (day 5 to 7) was not mirrored by an increase in protein suggesting translational or post-translational control. Mitochondrial-encoded transcripts were at least 10-fold more abundant than nuclear-encoded transcripts. The pattern of transcript and protein abundance for uncoupling proteins displayed a trend similar to other respiratory proteins examined, implicating similar control mechanisms. The expression of alternative oxidase differed, increasing throughout development with protein peaking at day 20, perhaps suggesting a role in senescence. Overall, this study indicated that respiratory gene expression and protein abundance is co-ordinated with respiratory activity for most components but that some components, such as the F1beta subunit may be under discrete forms of regulation.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Daley DO,Considine MJ,Howell KA,Millar AH,Day DA,Whelan Jdoi
10.1023/a:1022502501373subject
Has Abstractpub_date
2003-03-01 00:00:00pages
745-55issue
5eissn
0167-4412issn
1573-5028journal_volume
51pub_type
杂志文章abstract：:Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005959203396
更新日期：1997-12-01 00:00:00
abstract：:A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005897921567
更新日期：1997-12-01 00:00:00
abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040137
更新日期：1986-03-01 00:00:00
abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006355510883
更新日期：2000-03-01 00:00:00
abstract：:A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using degenerate primers corresponding to a region of the mature protein sequence and the 'rapid amplification o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020813
更新日期：1996-03-01 00:00:00
abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015034
更新日期：1987-05-01 00:00:00
abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00024201
更新日期：1994-04-01 00:00:00
abstract：:Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006118304093
更新日期：1999-04-01 00:00:00
abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006052108468
更新日期：1998-11-01 00:00:00
abstract：:To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005839832096
更新日期：1997-05-01 00:00:00
abstract：KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00831-z
更新日期：2019-03-01 00:00:00
abstract：:We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020244
更新日期：1995-05-01 00:00:00
abstract：:The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/b:plan.0000004333.96451.11
更新日期：2003-08-01 00:00:00
abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1011848528377
更新日期：2001-11-01 00:00:00
abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019477
更新日期：1996-07-01 00:00:00
abstract：:The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-005-7703-9
更新日期：2006-04-01 00:00:00
abstract：:Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016019
更新日期：1989-12-01 00:00:00
abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006099512706
更新日期：1998-06-01 00:00:00
abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019182
更新日期：1995-01-01 00:00:00
abstract：:The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021430
更新日期：1993-10-01 00:00:00
abstract：:We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006375123496
更新日期：1999-11-01 00:00:00
abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1021266320877
更新日期：2002-12-01 00:00:00
abstract：:Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019191
更新日期：1995-01-01 00:00:00
abstract：:Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9281-5
更新日期：2008-03-01 00:00:00
abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4922-z
更新日期：2006-03-01 00:00:00
abstract：:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006496308160
更新日期：2000-04-01 00:00:00
abstract：:Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029606
更新日期：1994-07-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：:β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9865-y
更新日期：2012-02-01 00:00:00
abstract：:The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0395-x
更新日期：2016-01-01 00:00:00
