Differential screening of mitochondrial cDNA libraries from male-fertile and cytoplasmic male-sterile sugar-beet reveals genome rearrangements at atp6 and atpA loci.


:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clones corresponding to structural genes. In addition, reciprocal hybridization experiments were performed using labelled first-strand cDNA to identify uniquely transcribed sequences. One cDNA clone (pYC700) is unique to CMS mitochondria and is located upstream of the F0F1-ATPase subunit 6 gene (atp6). Another cDNA clone (pYC130), when used as a probe in northern hybridization analysis, revealed novel transcript profiles in CMS sugar-beet mitochondria. Sequence analysis of this cDNA showed strong homology with the F0F1-ATPase subunit alpha (atpA) coding sequences from several higher plants. The atp6 and atpA loci from each genotype were cloned and the genomic organization, DNA sequence and transcription of each locus was studied. Differences in the transcript profiles of each gene are a consequence of genomic rearrangements 5' to the coding sequence.


Plant Mol Biol


Plant molecular biology


Xue Y,Collin S,Davies DR,Thomas CM




Has Abstract


1994-04-01 00:00:00












  • Molecular details of tomato extensin and glycine-rich protein gene expression.

    abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Showalter AM,Butt AD,Kim S

    更新日期:1992-05-01 00:00:00

  • Cloning of a cDNA encoding a 3-dehydroquinate synthase from a higher plant, and analysis of the organ-specific and elicitor-induced expression of the corresponding gene.

    abstract::cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

    更新日期:1996-04-01 00:00:00

  • Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat.

    abstract:KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Casartelli A,Melino VJ,Baumann U,Riboni M,Suchecki R,Jayasinghe NS,Mendis H,Watanabe M,Erban A,Zuther E,Hoefgen R,Roessner U,Okamoto M,Heuer S

    更新日期:2019-03-01 00:00:00

  • A genetic genomics-expression approach reveals components of the molecular mechanisms beyond the cell wall that underlie peach fruit woolliness due to cold storage.

    abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pons C,Martí C,Forment J,Crisosto CH,Dandekar AM,Granell A

    更新日期:2016-11-01 00:00:00

  • The Arabidopsis chloroplast RNase J displays both exo- and robust endonucleolytic activities.

    abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Halpert M,Liveanu V,Glaser F,Schuster G

    更新日期:2019-01-01 00:00:00

  • Identification and analysis of an outer-seed-coat-specific promoter from Arabidopsis thaliana.

    abstract::Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GW

    更新日期:2013-01-01 00:00:00

  • Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops.

    abstract::The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

    更新日期:2014-11-01 00:00:00

  • Structure and expression of the barley lipid transfer protein gene Ltp1.

    abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

    更新日期:1992-02-01 00:00:00

  • Molecular analysis of early rice stamen development using organ-specific gene expression profiling.

    abstract::Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

    更新日期:2006-08-01 00:00:00

  • A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum.

    abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

    更新日期:1993-03-01 00:00:00

  • Ectopic expression of VlmybA1 in grapevine activates a narrow set of genes involved in anthocyanin synthesis and transport.

    abstract::The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Cutanda-Perez MC,Ageorges A,Gomez C,Vialet S,Terrier N,Romieu C,Torregrosa L

    更新日期:2009-04-01 00:00:00

  • Expression characteristics of OS-ACS1 and OS-ACS2, two members of the 1-aminocyclopropane-1-carboxylate synthase gene family in rice (Oryza sativa L. cv. Habiganj Aman II) during partial submergence.

    abstract::Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zarembinski TI,Theologis A

    更新日期:1997-01-01 00:00:00

  • A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities.

    abstract::An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lopato S,Gattoni R,Fabini G,Stevenin J,Barta A

    更新日期:1999-03-01 00:00:00

  • Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.

    abstract::The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Xiang C,Miao ZH,Lam E

    更新日期:1996-11-01 00:00:00

  • Organization, not duplication, triggers silencing in a complex transgene locus in rice.

    abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

    更新日期:2005-06-01 00:00:00

  • Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

    abstract::In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Conceição Ada S,Van Vliet A,Krebbers E

    更新日期:1994-11-01 00:00:00

  • Transaldolase genes from the cyanobacteria Anabaena variabilis and Synechocystis sp. PCC 6803: comparison with other eubacterial and eukaryotic homologues.

    abstract::We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Köhler U,Cerff R,Brinkmann H

    更新日期:1996-01-01 00:00:00

  • Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte.

    abstract::A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

    更新日期:1999-07-01 00:00:00

  • TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro transcription system from Nicotiana tabacum.

    abstract::This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

    更新日期:1998-12-01 00:00:00

  • Over-expressing a yeast ornithine decarboxylase gene in transgenic roots of Nicotiana rustica can lead to enhanced nicotine accumulation.

    abstract::Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Hamill JD,Robins RJ,Parr AJ,Evans DM,Furze JM,Rhodes MJ

    更新日期:1990-07-01 00:00:00

  • Characterization, subcellular localization and nuclear targeting of casein kinase 2 from Zea mays.

    abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Peracchia G,Jensen AB,Culiáñez-Macià FA,Grosset J,Goday A,Issinger OG,Pagès M

    更新日期:1999-05-01 00:00:00

  • Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice.

    abstract::Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sharma R,Tan F,Jung KH,Sharma MK,Peng Z,Ronald PC

    更新日期:2011-11-01 00:00:00

  • Characterization of a mRNA that accumulates during development of oilseed rape pods.

    abstract::Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Coupe SA,Taylor JE,Isaac PG,Roberts JA

    更新日期:1994-01-01 00:00:00

  • The Arabidopsis thaliana GSK3/Shaggy like kinase AtSK3-2 modulates floral cell expansion.

    abstract::The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Claisse G,Charrier B,Kreis M

    更新日期:2007-05-01 00:00:00

  • Mesophyll-specific, light and metabolic regulation of the C4 PPCZm1 promoter in transgenic maize.

    abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kausch AP,Owen TP Jr,Zachwieja SJ,Flynn AR,Sheen J

    更新日期:2001-01-01 00:00:00

  • Comparative sequence analysis of cis elements present in Glycine max L. leghemoglobin lba and lbc3 genes.

    abstract::The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: She Q,Sandal NN,Stougaard J,Marcker KA

    更新日期:1993-08-01 00:00:00

  • Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis.

    abstract::Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Hsieh MH,Chang CY,Hsu SJ,Chen JJ

    更新日期:2008-04-01 00:00:00

  • A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and physiological responses.

    abstract::A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Deng X,Phillips J,Bräutigam A,Engström P,Johannesson H,Ouwerkerk PB,Ruberti I,Salinas J,Vera P,Iannacone R,Meijer AH,Bartels D

    更新日期:2006-06-01 00:00:00

  • The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner.

    abstract::The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Urquhart W,Gunawardena AH,Moeder W,Ali R,Berkowitz GA,Yoshioka K

    更新日期:2007-12-01 00:00:00

  • Genomic organization and evolution of the soybean SB92 satellite sequence.

    abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Vahedian M,Shi L,Zhu T,Okimoto R,Danna K,Keim P

    更新日期:1995-11-01 00:00:00