Differential screening of mitochondrial cDNA libraries from male-fertile and cytoplasmic male-sterile sugar-beet reveals genome rearrangements at atp6 and atpA loci.

abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...

journal_title：Plant molecular biology

authors： Huang J,Levings CS 3rd

更新日期：1995-11-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

journal_title：Plant molecular biology

authors： Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

更新日期：1987-11-01 00:00:00

abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...

journal_title：Plant molecular biology

authors： Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

更新日期：2014-11-01 00:00:00

abstract：KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...

journal_title：Plant molecular biology

authors： Liu JJ,Williams H,Li XR,Schoettle AW,Sniezko RA,Murray M,Zamany A,Roke G,Chen H

更新日期：2017-11-01 00:00:00

abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

journal_title：Plant molecular biology

authors： Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

更新日期：1996-12-01 00:00:00

abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...

journal_title：Plant molecular biology

authors： Li YZ,Pan YH,Sun CB,Dong HT,Luo XL,Wang ZQ,Tang JL,Chen B

更新日期：2010-12-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...

journal_title：Plant molecular biology

authors： Smith CJ,Watson CF,Morris PC,Bird CR,Seymour GB,Gray JE,Arnold C,Tucker GA,Schuch W,Harding S

更新日期：1990-03-01 00:00:00

abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...

journal_title：Plant molecular biology

authors： Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

更新日期：1993-05-01 00:00:00

abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

journal_title：Plant molecular biology

authors： Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

更新日期：2011-08-01 00:00:00

abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...

journal_title：Plant molecular biology

authors： Loader NM,Woolner EM,Hellyer A,Slabas AR,Safford R

更新日期：1993-11-01 00:00:00

abstract：:Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...

journal_title：Plant molecular biology

authors： Johanningmeier U,Heiss S

更新日期：1993-04-01 00:00:00

abstract：:Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...

journal_title：Plant molecular biology

authors： Nakagawa A,Takahashi H,Kojima S,Sato N,Ohga K,Cha BY,Woo JT,Nagai K,Horiguchi G,Tsukaya H,Machida Y,Machida C

更新日期：2012-08-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

journal_title：Plant molecular biology

authors： Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

更新日期：2011-02-01 00:00:00

abstract：:Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...

journal_title：Plant molecular biology

authors： Poage M,Le Martret B,Jansen MA,Nugent GD,Dix PJ

更新日期：2011-07-01 00:00:00

abstract：:Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...

journal_title：Plant molecular biology

authors： Valdés AE,Overnäs E,Johansson H,Rada-Iglesias A,Engström P

更新日期：2012-11-01 00:00:00

abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...

journal_title：Plant molecular biology

authors： Wu H,Liu XQ

更新日期：1997-05-01 00:00:00

abstract：:Rice storage protein glutelin genes are coordinately regulated during seed development. A previous 5' deletion analysis using transgenic tobacco revealed that the minimum 5' region necessary for endosperm specificity was within -245 bp of the transcription start site, and included the AACA and GCN4 motifs that are hig...

journal_title：Plant molecular biology

authors： Washida H,Wu CY,Suzuki A,Yamanouchi U,Akihama T,Harada K,Takaiwa F

更新日期：1999-05-01 00:00:00

abstract：:Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expre...

journal_title：Plant molecular biology

authors： Gao MJ,Parkin I,Lydiate D,Hannoufa A

更新日期：2004-05-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...

journal_title：Plant molecular biology

authors： Maiti MK,Krishnasamy S,Owen HA,Makaroff CA

更新日期：1997-11-01 00:00:00

abstract：:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

journal_title：Plant molecular biology

authors： Turek I,Gehring C

更新日期：2016-06-01 00:00:00

abstract：:Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...

journal_title：Plant molecular biology

authors： Paul AL,Ferl RJ

更新日期：1993-09-01 00:00:00

abstract：:Galactomannans are hemicellulosic polysaccharides composed of a (1 → 4)-linked β-D-mannan backbone substituted with single-unit (1 → 6)-α-linked D-galactosyl residues. Developing fenugreek (Trigonella foenum-graecum) seeds are known to accumulate large quantities of galactomannans in the endosperm, and were thus used ...

journal_title：Plant molecular biology

authors： Wang Y,Alonso AP,Wilkerson CG,Keegstra K

更新日期：2012-06-01 00:00:00

abstract：:The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the who...

journal_title：Plant molecular biology

authors： Suoniemi A,Anamthawat-Jónsson K,Arna T,Schulman AH

更新日期：1996-03-01 00:00:00

abstract：:A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...

journal_title：Plant molecular biology

authors： Nakajima T,Suzůki A,Tanifuji S,Kato A

更新日期：1992-12-01 00:00:00

abstract：:A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...

journal_title：Plant molecular biology

authors： Morgens PH,Callahan AM,Dunn LJ,Abeles FB

更新日期：1990-05-01 00:00:00

abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

journal_title：Plant molecular biology

authors： Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

更新日期：2003-06-01 00:00:00