Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome.

abstract：:Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...

journal_title：Plant molecular biology

authors： Fukuda N,Ikawa Y,Aoyagi T,Kozaki A

更新日期：2013-07-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...

journal_title：Plant molecular biology

authors： Mock HP,Trainotti L,Kruse E,Grimm B

更新日期：1995-05-01 00:00:00

abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

journal_title：Plant molecular biology

authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

更新日期：2008-11-01 00:00:00

abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

journal_title：Plant molecular biology

authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

更新日期：2001-08-01 00:00:00

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and i...

journal_title：Plant molecular biology

authors： Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein H

更新日期：1994-10-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

journal_title：Plant molecular biology

authors： Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

更新日期：2014-01-01 00:00:00

abstract：:Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...

journal_title：Plant molecular biology

authors： Menges M,Hennig L,Gruissem W,Murray JA

更新日期：2003-11-01 00:00:00

abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...

journal_title：Plant molecular biology

authors： Pechan T,Jiang B,Steckler D,Ye L,Lin L,Luthe DS,Williams WP

更新日期：1999-05-01 00:00:00

abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...

journal_title：Plant molecular biology

authors： Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

更新日期：2014-11-01 00:00:00

abstract：:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...

journal_title：Plant molecular biology

authors： O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JC

更新日期：1995-02-01 00:00:00

abstract：:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

journal_title：Plant molecular biology

authors： Coupe SA,Taylor JE,Isaac PG,Roberts JA

更新日期：1994-01-01 00:00:00

abstract：:Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that ...

journal_title：Plant molecular biology

authors： Vershinin AV,Heslop-Harrison JS

更新日期：1998-01-01 00:00:00

abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

journal_title：Plant molecular biology

authors： Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

更新日期：2020-08-01 00:00:00

abstract：:The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

journal_title：Plant molecular biology

authors： Montoliu L,Rigau J,Puigdomènech P

更新日期：1990-01-01 00:00:00

abstract：:β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

journal_title：Plant molecular biology

authors： Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

更新日期：2012-02-01 00:00:00

abstract：:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

journal_title：Plant molecular biology

authors： Conceição Ada S,Van Vliet A,Krebbers E

更新日期：1994-11-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00

abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

journal_title：Plant molecular biology

authors： Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

更新日期：1999-05-01 00:00:00

abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

journal_title：Plant molecular biology

authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

更新日期：1992-01-01 00:00:00

abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

journal_title：Plant molecular biology

authors： Temple SJ,Bagga S,Sengupta-Gopalan C

更新日期：1998-06-01 00:00:00

abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

journal_title：Plant molecular biology

authors： Devitt ML,Maas KJ,Stafstrom JP

更新日期：1999-01-01 00:00:00

abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...

journal_title：Plant molecular biology

authors： Kohno-Murase J,Murase M,Ichikawa H,Imamura J

更新日期：1994-11-01 00:00:00

abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

journal_title：Plant molecular biology

authors： Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

更新日期：2011-10-01 00:00:00

abstract：:A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the o...

journal_title：Plant molecular biology

authors： Luschnig C,Bachmair A,Schweizer D

更新日期：1993-06-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...

journal_title：Plant molecular biology

authors： Kato A,Nakajima T,Yamashita J,Yakura K,Tanifuji S

更新日期：1990-06-01 00:00:00