Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley.

abstract：:The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...

journal_title：Plant molecular biology

authors： Voronin V,Touraev A,Kieft H,van Lammeren AA,Heberle-Bors E,Wilson C

更新日期：2001-04-01 00:00:00

abstract：:DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus ...

journal_title：Plant molecular biology

authors： Hess WR,Weihe A,Loiseaux-de Goër S,Partensky F,Vaulot D

更新日期：1995-03-01 00:00:00

abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

journal_title：Plant molecular biology

authors： Jackson MT

更新日期：1997-09-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

journal_title：Plant molecular biology

authors： Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

更新日期：1991-10-01 00:00:00

abstract：:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...

journal_title：Plant molecular biology

authors： Gupta R,Webster CI,Gray JC

更新日期：1998-04-01 00:00:00

abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...

journal_title：Plant molecular biology

authors： Hu J,Reddy VS,Wessler SR

更新日期：2000-03-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...

journal_title：Plant molecular biology

authors： Ellerström M,Stålberg K,Ezcurra I,Rask L

更新日期：1996-12-01 00:00:00

abstract：:Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...

journal_title：Plant molecular biology

authors： Raj-Kumar PK,Vallon O,Liang C

更新日期：2017-06-01 00:00:00

abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...

journal_title：Plant molecular biology

authors： van Klaveren P,Slats J,Roosien J,van Vloten-Doting L

更新日期：1983-01-01 00:00:00

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00

abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

journal_title：Plant molecular biology

authors： Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

更新日期：1992-02-01 00:00:00

abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...

journal_title：Plant molecular biology

authors： Kirchherr D,Albrecht H,Mesnard JM,Lebeurier G

更新日期：1988-05-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

journal_title：Plant molecular biology

authors： Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

更新日期：2011-02-01 00:00:00

abstract：:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...

journal_title：Plant molecular biology

authors： Sonti RV,Tissier AF,Wong D,Viret JF,Signer ER

更新日期：1995-09-01 00:00:00

abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

journal_title：Plant molecular biology

authors： Rose AB,Carter A,Korf I,Kojima N

更新日期：2016-10-01 00:00:00

abstract：:Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...

journal_title：Plant molecular biology

authors： Li AL,Zhu YF,Tan XM,Wang X,Wei B,Guo HZ,Zhang ZL,Chen XB,Zhao GY,Kong XY,Jia JZ,Mao L

更新日期：2008-03-01 00:00:00

abstract：:The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches are used to detect the interactions between a transcription factor (TF) and DNA, i.e. the TF-centered or protein-DNA approach, and the gene-centered or DN...

journal_title：Plant molecular biology

authors： Ji X,Wang L,Nie X,He L,Zang D,Liu Y,Zhang B,Wang Y

更新日期：2014-11-01 00:00:00

abstract：:In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...

journal_title：Plant molecular biology

authors： Hirschi KD,Miranda ML,Wilganowski NL

更新日期：2001-05-01 00:00:00

abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

journal_title：Plant molecular biology

authors： Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

更新日期：2005-02-01 00:00:00

abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...

journal_title：Plant molecular biology

authors： Cervantes E,Rodríguez A,Nicolás G

更新日期：1994-05-01 00:00:00

abstract：:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...

journal_title：Plant molecular biology

authors： Kuroda I,Inagaki J,Yamamoto Y

更新日期：1993-01-01 00:00:00

abstract：KEY MESSAGE:Two OsDGAT1 genes showed the ability to restore TAG and LB synthesis in yeast H1246. Alterations in the N-terminal region of OsDGAT1-1 gene revealed its regulatory role in gene function. Accumulation of triacylglycerol (TAG) or oil in vegetative tissues has emerged as a promising approach to meet the global...

journal_title：Plant molecular biology

authors： Bhunia RK,Sinha K,Chawla K,Randhawa V,Sharma TR

更新日期：2020-10-21 00:00:00

abstract：:Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...

journal_title：Plant molecular biology

authors： Xie Y,Wu R

更新日期：1989-07-01 00:00:00

abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...

journal_title：Plant molecular biology

authors： Fraley RT,Horsch RB,Matzke A,Chilton MD,Chilton WS,Sanders PR

更新日期：1984-11-01 00:00:00

abstract：:Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...

journal_title：Plant molecular biology

authors： Takaiwa F,Yamanouchi U,Yoshihara T,Washida H,Tanabe F,Kato A,Yamada K

更新日期：1996-03-01 00:00:00

abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...

journal_title：Plant molecular biology

authors： Loader NM,Woolner EM,Hellyer A,Slabas AR,Safford R

更新日期：1993-11-01 00:00:00

abstract：:Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...

journal_title：Plant molecular biology

authors： Chaal BK,Ishida K,Green BR

更新日期：2003-05-01 00:00:00

abstract：:Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis....

journal_title：Plant molecular biology

authors： Benvenuto E,Ordàs RJ,Tavazza R,Ancora G,Biocca S,Cattaneo A,Galeffi P

更新日期：1991-10-01 00:00:00