Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.

abstract：:Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...

journal_title：Plant molecular biology

authors： Prassinos C,Haralampidis K,Milioni D,Samakovli D,Krambis K,Hatzopoulos P

更新日期：2008-07-01 00:00:00

abstract：:Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...

journal_title：Plant molecular biology

authors： Gong Z,Yamazaki M,Sugiyama M,Tanaka Y,Saito K

更新日期：1997-12-01 00:00:00

abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...

journal_title：Plant molecular biology

authors： Piechulla B,Merforth N,Rudolph B

更新日期：1998-11-01 00:00:00

abstract：:We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...

journal_title：Plant molecular biology

authors： Valentin K,Maid U,Emich A,Zetsche K

更新日期：1992-10-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...

journal_title：Plant molecular biology

authors： Chung KM,Sano H

更新日期：2007-12-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

journal_title：Plant molecular biology

authors： Leah R,Mundy J

更新日期：1989-06-01 00:00:00

abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...

journal_title：Plant molecular biology

authors： Cervantes E,Rodríguez A,Nicolás G

更新日期：1994-05-01 00:00:00

abstract：:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...

journal_title：Plant molecular biology

authors： Hellens RP,Edwards EA,Leyland NR,Bean S,Mullineaux PM

更新日期：2000-04-01 00:00:00

abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

journal_title：Plant molecular biology

authors： Panpetch P,Field RA,Limpaseni T

更新日期：2018-03-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...

journal_title：Plant molecular biology

authors： Schmidt K,Heberle B,Kurrasch J,Nehls R,Stahl DJ

更新日期：2004-08-01 00:00:00

abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...

journal_title：Plant molecular biology

authors： Ioset JR,Urbaniak B,Ndjoko-Ioset K,Wirth J,Martin F,Gruissem W,Hostettmann K,Sautter C

更新日期：2007-11-01 00:00:00

abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

journal_title：Plant molecular biology

authors： Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

更新日期：2014-01-01 00:00:00

abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...

journal_title：Plant molecular biology

authors： Kanneganti V,Gupta AK

更新日期：2008-03-01 00:00:00

abstract：:As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...

journal_title：Plant molecular biology

authors： Odell JT,Knowlton S,Lin W,Mauvais CJ

更新日期：1988-05-01 00:00:00

abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...

journal_title：Plant molecular biology

authors： Richard C,Ouellet H,Guertin M

更新日期：2000-01-01 00:00:00

abstract：:This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

journal_title：Plant molecular biology

authors： Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

更新日期：1998-12-01 00:00:00

abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

journal_title：Plant molecular biology

authors： Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

更新日期：1999-07-01 00:00:00

abstract：:Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas ...

journal_title：Plant molecular biology

authors： Petersen JL,Lang DW,Small GD

更新日期：1999-08-01 00:00:00

abstract：KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...

journal_title：Plant molecular biology

authors： Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

更新日期：2021-01-22 00:00:00

abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

journal_title：Plant molecular biology

authors： Ward GC,Levings CS 3rd

更新日期：1991-11-01 00:00:00

abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...

journal_title：Plant molecular biology

authors： Palauqui JC,Vaucheret H

更新日期：1995-10-01 00:00:00

abstract：:In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...

journal_title：Plant molecular biology

authors： Allison LA,Kiss GB,Bauer P,Poiret M,Pierre M,Savouré A,Kondorosi E,Kondorosi A

更新日期：1993-01-01 00:00:00

abstract：:The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...

journal_title：Plant molecular biology

authors： Ogita S,Uefuji H,Morimoto M,Sano H

更新日期：2004-04-01 00:00:00

abstract：:We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...

journal_title：Plant molecular biology

authors： Rohrmeier T,Lehle L

更新日期：1993-08-01 00:00:00

abstract：:The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...

journal_title：Plant molecular biology

authors： Claisse G,Charrier B,Kreis M

更新日期：2007-05-01 00:00:00

abstract：:Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...

journal_title：Plant molecular biology

authors： Capone I,Frugis G,Costantino P,Cardarelli M

更新日期：1994-07-01 00:00:00

abstract：:The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...

journal_title：Plant molecular biology

authors： Cutanda-Perez MC,Ageorges A,Gomez C,Vialet S,Terrier N,Romieu C,Torregrosa L

更新日期：2009-04-01 00:00:00

abstract：:The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...

journal_title：Plant molecular biology

authors： Guan L,Polidoros AN,Scandalios JG

更新日期：1996-03-01 00:00:00

abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

journal_title：Plant molecular biology

authors： Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

更新日期：2006-08-01 00:00:00