In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains.

abstract：:We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...

journal_title：Plant molecular biology

authors： Kurkela S,Franck M

更新日期：1990-07-01 00:00:00

abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

journal_title：Plant molecular biology

authors： Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

更新日期：2014-01-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...

journal_title：Plant molecular biology

authors： Tilton GB,Wedemeyer WJ,Browse J,Ohlrogge J

更新日期：2006-07-01 00:00:00

abstract：:An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesi...

journal_title：Plant molecular biology

authors： Hansen G,Tempé J,Brevet J

更新日期：1992-10-01 00:00:00

abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

journal_title：Plant molecular biology

authors： Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

更新日期：2005-02-01 00:00:00

abstract：:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the exp...

journal_title：Plant molecular biology

authors： de Pater S,Hensgens LA,Schilperoort RA

更新日期：1990-09-01 00:00:00

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

journal_title：Plant molecular biology

authors： McNeilly D,Schofield A,Stone SL

更新日期：2018-02-01 00:00:00

abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

journal_title：Plant molecular biology

authors： Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

更新日期：1996-04-01 00:00:00

abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

journal_title：Plant molecular biology

authors： Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

更新日期：1996-12-01 00:00:00

abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

journal_title：Plant molecular biology

authors： Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

更新日期：2020-09-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...

journal_title：Plant molecular biology

authors： Neininger A,Seith B,Hoch B,Mohr H

更新日期：1994-06-01 00:00:00

abstract：:Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

journal_title：Plant molecular biology

authors： Takahashi R,Joshee N,Kitagawa Y

更新日期：1994-10-01 00:00:00

abstract：:Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2%...

journal_title：Plant molecular biology

authors： Chung YY,Kim SR,Finkel D,Yanofsky MF,An G

更新日期：1994-10-01 00:00:00

abstract：:Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...

journal_title：Plant molecular biology

authors： Waldie T,Hayward A,Beveridge CA

更新日期：2010-05-01 00:00:00

abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...

journal_title：Plant molecular biology

authors： de Silva J,Robinson SJ,Safford R

更新日期：1992-04-01 00:00:00

abstract：:Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...

journal_title：Plant molecular biology

authors： Sharma R,Tan F,Jung KH,Sharma MK,Peng Z,Ronald PC

更新日期：2011-11-01 00:00:00

abstract：:The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...

journal_title：Plant molecular biology

authors： Hong SY,Lee TS,Kim J,Jung JH,Choi CW,Kim TG,Kwon TH,Jang YS,Yang MS

更新日期：2008-11-01 00:00:00

abstract：:We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

journal_title：Plant molecular biology

authors： Makaroff CA,Apel IJ,Palmer JD

更新日期：1990-11-01 00:00:00

abstract：:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...

journal_title：Plant molecular biology

authors： Pelzer-Reith B,Penger A,Schnarrenberger C

更新日期：1993-01-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

journal_title：Plant molecular biology

authors： Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

更新日期：2020-01-01 00:00:00

abstract：:A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...

journal_title：Plant molecular biology

authors： Petridou S,Foster K,Kindle K

更新日期：1997-02-01 00:00:00

abstract：:Target sequence cleavage is the essential step for intron invasion into an intronless allele. DNA cleavage at a specific site is performed by an endonuclease, termed a homing enzyme, which is encoded by an open reading frame within the intron. The recognition properties of them have only been analyzed in vitro, using ...

journal_title：Plant molecular biology

authors： Kurokawa S,Yamasaki T,Komatsu T,Watanabe KI,Ohama T

更新日期：2006-09-01 00:00:00

abstract：:Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...

journal_title：Plant molecular biology

authors： Burow M,Bergner A,Gershenzon J,Wittstock U

更新日期：2007-01-01 00:00:00

abstract：:The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...

journal_title：Plant molecular biology

authors： Kato A,Nakajima T,Yamashita J,Yakura K,Tanifuji S

更新日期：1990-06-01 00:00:00

abstract：:Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

journal_title：Plant molecular biology

authors： Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

更新日期：2010-12-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00